Multi-isotopic and morphometric evidence for the migration of farmers leading up to the Inka conquest of the southern Andes.

We present isotopic and morphometric evidence suggesting the migration of farmers in the southern Andes in the period AD 1270-1420, leading up to the Inka conquest occurring ~ AD 1400. This is based on the interdisciplinary study of human remains from archaeological cemeteries in the Andean Uspallata Valley (Argentina), located in the southern frontier of the Inka Empire. The studied samples span AD 800-1500, encompassing the highly dynamic Late Intermediate Period and culminating with the imperial expansion. Our research combines a macro-regional study of human paleomobility and migration based on a new strontium isoscape across the Andes that allows identifying locals and migrants, a geometric morphometric analysis of cranio-facial morphology suggesting separate ancestral lineages, and a paleodietary reconstruction based on stable isotopes showing that the migrants had diets exceptionally high in C4 plants and largely based on maize agriculture. Significantly, this migration influx occurred during a period of regional demographic increase and would have been part of a widespread period of change in settlement patterns and population movements that preceded the Inka expansion. These processes increased local social diversity and may have been subsequently utilized by the Inka to channel interaction with the local societies.

Scale of human mobility in the southern Andes (Argentina and Chile): A new framework based on strontium isotopes.

OBJECTIVES: The goal of this article is to assess the scale of human paleomobility and ecological complementarity between the lowlands and highlands in the southern Andes during the last 2,300 years. By providing isotope results for human bone and teeth samples, we assess a hypothesis of "high residential mobility" suggested on the basis of oxygen isotopes from human remains. METHODS: We develop an isotopic assessment of human mobility in a mountain landscape combining strontium and oxygen isotopes. We analyze bone and teeth samples as an approach to life-history changes in spatial residence. Human samples from the main geological units and periods within the last two millennia are selected. RESULTS: We present a framework for the analysis of bioavailable strontium based on the combination of the geological data with isotope results for rodent samples. The 87 Sr/86 Sr values from human samples indicate residential stability within geological regions along life history. When comparing strontium and oxygen values for the same human samples, we record a divergent pattern: while δ18 O values for samples from distant regions overlap widely, there are important differences in 87 Sr/86 Sr values. CONCLUSIONS: Despite the large socio-economic changes recorded, 87 Sr/86 Sr values indicate a persisting scenario of low systematic mobility between the different geological regions. Our results suggest that strontium isotope values provide the most germane means to track patterns of human occupation of distinct regions in complex geological landscapes, offering a much higher spatial resolution than oxygen isotopes in the southern Andes.

Effect of bite force and diet composition on craniofacial diversification of Southern South American human populations.

Ecological factors can be important to shape the patterns of morphological variation among human populations. Particularly, diet plays a fundamental role in craniofacial variation due to both the effect of the nutritional status-mostly dependent on the type and amount of nutrients consumed-on skeletal growth and the localized effects of masticatory forces. We examine these two dimensions of diet and evaluate their influence on morphological diversification of human populations from southern South America during the late Holocene. Cranial morphology was measured as 3D coordinates defining the face, base and vault. Size, form, and shape variables were obtained for 474 adult individuals coming from 12 samples. Diet composition was inferred from carious lesions and δ(13) C data, whereas bite forces were estimated using traits of main jaw muscles. The spatial structure of the morphological and ecological variables was measured using correlograms. The influence of diet composition and bite force on morphometric variation was estimated by a spatial regression model. Cranial variation and diet composition display a geographical structure, while no geographical pattern was observed in bite forces. Cranial variation in size and form is significantly associated with diet composition, suggesting a strong effect of systemic factors on cranial growth. Conversely, bite forces do not contribute significantly to the pattern of morphological variation among the samples analyzed. Overall, these results show that an association between diet composition and hardness cannot be assumed, and highlight the complex relationship between morphological diversification and diet in human populations.

A palaeopathological example of Legg-Calvé-Perthes disease from Argentina.

Legg-Calvé-Perthes (LCP) disease is a very uncommon condition that affects one in 10,000 paediatric patients. Examples described in the palaeopathological literature are scarce and for this reason the exceptional examples found in archaeological contexts are worthy of analysis, description and reporting. We record an archaeological example from Argentina, skeleton 12 Cápiz Alto (12 CA) that shows skeletal signs compatible with this condition.

Role of wild plant foods among late Holocene hunter-gatherers from Central and North Patagonia (South America): an approach from dental evidence.

This study evaluates the role of plant foods in the subsistence of hunter-gatherers that inhabited the Central East, Northwest, and Northeast Patagonia (Argentina) during the late Holocene (ca. 3,000-500 years BP). The goal of the present study is to assess the temporal variation of dental caries ratio and wear rate in skeletal samples to ascertain if the biological information supports the dietary shift toward greater consumption of wild plant foods around 1,500 years BP, suggested by other types of evidence. The authors registered caries, antemortem and postmortem tooth loss, and tooth wear from eight samples belonging to hunter-gatherers from Patagonia for which chronological sequences from early late Holocene (ca. 3,000-1,500 years BP) up to final late Holocene (ca. 1,500-500 years BP) are available. The results indicate that caries percentages in Patagonian samples fall within the range established for hunter-gatherers but there are significant geographical differences. In addition, caries ratio does not change significantly through time, so the amount of carbohydrates consumed seems to have remained fairly constant since 3,000 years BP. In contrast, there is a marked temporal trend toward the reduction of wear rates in the three areas, suggesting a faster rate in early late Holocene than in final late Holocene. These results would reflect a change to less hard and/or abrasive diets in the final late Holocene, probably owing to differences in food processing methods employed.

Craniofacial morphology in the Argentine Center-West: consequences of the transition to food production.

The Argentine Center-West was the southernmost portion of the Andes where domestication of plants and animals evolved. Populations located in the southern portion of this area displayed a hunter-gatherer subsistence economy up to historical times, and coexisted with farmers located to the north. Archaeological and biological evidence suggests that the transition to food production was associated with the consumption of a softer diet and a more sedentary way of life. This study tests the hypothesis that diet-related factors influenced morphological differentiation, by comparing functional cranial components of farmers and hunter-gatherers. Three-dimensional changes on eight minor functional components (anteroneural, midneural, posteroneural, otic, optic, respiratory, masticatory, and alveolar) were measured on skulls derived from both subareas. Volumetric and morphometric indices were calculated to estimate the absolute and relative size of components, respectively. Results of a paired t-test indicated that farmers have a smaller craniofacial size than hunter-gatherers. The components that varied the most were masticatory and posteroneural, showing smaller absolute and relative sizes in farmers. Discriminant analyses indicated that lengths and widths were the most affected dimensions of these and other components. The pattern of differentiation, which involves specific components, enabled us to exclude differential gene flow and stochastic mechanisms as the main causes. Instead, results support the hypothesis that diet-related factors associated with both subsistence economies influenced craniofacial morphology. A proportion of the observed variation associated with size differences can be explained by two systemic factors: the lesser quality of nutrition due to a low protein content in the diet, and a decrease of growth hormone circulation induced by a lower mobility due to sedentism. However, differentiation is better explained by a localized factor: the reduction in the masticatory and posteroneural components in farmers resulted from a decrease of masticatory stresses and workload on the head and neck, linked to the consumption of a softer diet.