Stimulation and inhibition of respiratory burst in neutrophils as a result of action of weak combined magnetic fields adjusted to ICR of protonated water forms.

Weak combined collinear magnetic fields (CMFs) composed of constant component, 60 µT and low-frequency alternating field, 100 nT, used in 40 min pretreatment, were shown to have diverse effects (alleviation/stimulation) depending on the frequency of the alternating component on respiratory burst intensity in neutrophil suspension after activation by N-formyl-Met-Leu-Phe recorded by luminol-dependent chemiluminescence. About 12.6 Hz frequency formally corresponding to ion cyclotron resonance (ICR) frequency of hydrated hydronium ion (Н9О4 +) had notable stimulating effect. On the contrary, treatment with 48.5 Hz frequency corresponding to hydronium ion (Н3О+) was accompanied by significant alleviation of respiratory burst intensity. CMFs-pretreated water conducted only stimulating effect of CMFs when the field was adjusted to ICR of hydrated hydronium ion form, which is direct proof of participation of water in the mechanism of this effect of CMFs.

Effects of low-level combined static and weak low-frequency alternating magnetic fields on cytokine production and tumor development in mice.

We investigated the effects of weak combined magnetic fields (MFs) produced by superimposing a constant MF (in the range 30 - 150 µT) and an alternating MF (100 or 200 nT) on cytokine production in healthy Balb/C male mice exposed 2 h daily for 14 days. The alternating magnetic field was a sum of several frequencies (ranging from 2.5 - 17.5 Hz). The frequencies of the alternating magnetic field were calculated formally based on the cyclotron resonance of ions of free amino acids (glutamic and aspartic acids, arginine, lysine, histidine, and tyrosine). The selection of different intensity and frequency combinations of constant and alternating magnetic fields was performed to find the optimal characteristics for cytokine production stimulation in immune cells. MF with a constant component of 60 µT and an alternating component of 100 nT, which was a sum of six frequencies (from 5 to 7 Hz), was found to stimulate the production of tumor necrosis factor-α, interferon-gamma, interleukin-2, and interleukin-3 in healthy mouse cells and induce cytokine accumulation in blood plasma. Then, we studied the effect of this MF on tumor-bearing mice with solid tumors induced by Ehrlich ascite carcinoma cells by observing tumor development processes, including tumor size, mouse survival rate, and average lifespan. Tumor-bearing mice exposed to a combined constant magnetic field of 60 µT and an alternating magnetic field of 100 nT containing six frequencies showed a strong suppression of tumor growth with an increase in survival rate and enhancement of average lifespan.

Effect of weak combined static and extremely low-frequency alternating magnetic fields on spatial memory and brain amyloid-ß in two animal models of Alzheimer's disease.

Subchronic effect of a weak combined magnetic field (MF), produced by superimposing a constant component, 42 µT and an alternating MF of 0.08 µT, which was the sum of two frequencies of 4.38 and 4.88 Hz, was studied in olfactory bulbectomized (OBE) and transgenic Tg (APPswe, PSEN1) mice, which were used as animal models of sporadic and heritable Alzheimer's disease (AD) accordingly. Spatial memory was tested in a Morris water maze on the following day after completion of training trials with the hidden platform removed. The amyloid-ß (Aß) level was determined in extracts of the cortex and hippocampus of mice using a specific DOT analysis while the number and dimensions of amyloid plaques were detected after their staining with thioflavin S in transgenic animals. Exposure to the MFs (4 h/day for 10 days) induced the decrease of Aß level in brain of OBE mice and reduced the number of Aß plaques in the cortex and hippocampus of Tg animals. However, memory improvement was revealed in Tg mice only, but not in the OBE animals. Here, we suggest that in order to prevent the Aß accumulation, MFs could be used at early stage of neuronal degeneration in case of AD and other diseases with amyloid protein deposition in other tissues.

Effect of weak combined static and extremely low-frequency alternating magnetic fields on tumor growth in mice inoculated with the Ehrlich ascites carcinoma.

It has been shown that the ultralow-frequency extremely weak alternating component of combined magnetic fields (MFs) exhibits a marked antitumor activity. The parameters of this component have been found (frequency 1, 4.4, 16.5 Hz or the sum of these frequencies; intensity 300, 100, 150-300 nT, respectively) at which this MF in combination with a collinear static MF of 42 microT inhibits or suppresses the growth of Ehrlich ascites carcinoma (EAC) in mice. It was shown that the exposure of mice with EAC to combined MFs causes structural changes in some organs (liver, adrenal glands), which are probably due to the total degradation of the tumor tissue. In mice with transplanted EAC, the tumor tissue after exposure to weak MFs was practically absent, as distinct from control animals in which the invasion of the tumor into the adipose tissue surrounding the kidneys, mesenteric lymph nodes, and spermatic appendages was observed. In animals without tumors, no pathological deviations from the norm in the structure of organs and tissues occurred after exposure to weak MF, indicating that this factor per se is not toxic to the organism.

Effect of weak static and low-frequency alternating magnetic fields on the fission and regeneration of the planarian dugesia (Girardia) tigrina.

The effect of weak static (DC) and alternating (AC) magnetic fields (MFs), as well as combined (AC/DC) collinear MFs on the intensity of morphogenesis processes in the planarian Dugesia (Girardia) tigrina has been studied. It was found that combined MFs produce a stimulating effect on the fission and regeneration of planarians. Both components of the combined MFs, the direct (DC) and the alternating (AC), are important in the realization of the effects of weak MFs. The practically complete absence of one of the components (DC) reverses the sign of the effect. It was shown that the presence of concomitant background MFs does not substantially influence the effects of combined MFs with a very small AC component (100 nT). The effect of the "zero" field is significant and comparable in magnitude with the effects of combined MFs at effective frequencies. Narrow zones of effective amplitudes (in the region of tens and hundreds of nT) of the AC component of the combined MFs, with the DC component close to the value of the geomagnetic field were found, which alternate with regions where the response of the biological object to the influence is absent.