ABSTRACT
Stevia (Stevia rebaudiana), recognized for its low-calorie, sugar-free attributes, and various health benefits, has potential applications beyond human consumption, particularly in agriculture. This study explored the potential uses of Stevia in both agricultural and healthcare contexts by examining its plant-inhibitory, cytotoxic, and antioxidant effects. The methanolic extract of Stevia leaves was fractionated into hexane, ethyl acetate, chloroform, and water fractions. These fractions were then subjected to the bioassay analyses above and underwent identification of their chemical constituents. The results indicated that the ethyl acetate fraction demonstrated significant inhibitory effects on weed germination and growth of Beggars tick (Bidens frondosa) (100% inhibition at 1000 ppm of dose). This fraction also exhibited the highest antioxidant activity, total phenolic, and total flavonoid contents (IC50 DPPH = 18.67 µg/mL, 103.50 mg GAE/g fraction, and 410.16 mg QE/g fraction, respectively). In contrast, the chloroform fraction showed the highest cytotoxic effect (LC50 = 700.01 ppm) in the brine shrimp (Artemia salina) mortality evaluation. Pearson's correlation analysis revealed a positive correlation among plant inhibitory effects, antioxidant potentials, and phenolic/flavonoid contents of Stevia. FTIR spectra confirmed the presence of phenols and nonpolar components in the ethyl acetate and chloroform fractions. In addition, GC-MS analysis successfully identified Stevia's key constituents, including tetracontane, hexadecane, hexadecanoic acid, methyl ester in the ethyl acetate fraction, and spiro [4.5] decan-7-one and 6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one in the chloroform fraction. This study underscores the potential of S. rebaudiana as a source of natural antioxidants and herbicides, offering valuable insights into its diverse applications in agriculture.
