ABSTRACT
A novel constituent of bacterial polysaccharides, 2,3,4-triacetamido-2,3,4-trideoxy-L-arabinose, was found in the O-specific polysaccharide from the lipopolysaccharide of Psychrobacter cryohalolentis K5(T) and identified by 1D and 2D (1)H and (13)C NMR studies of the polysaccharide and a disaccharide obtained by solvolysis of the polysaccharide with triflic acid. The following structure of the branched polysaccharide was established by sugar analysis, triflic acid solvolysis, Smith degradation, and 2D NMR spectroscopy.
Subject(s)
Arabinose/analogs & derivatives , Lipopolysaccharides/chemistry , O Antigens/chemistry , Polysaccharides, Bacterial/chemistry , Psychrobacter/chemistry , Arabinose/chemistry , Molecular Structure , Nuclear Magnetic Resonance, BiomolecularABSTRACT
An acidic polysaccharide was obtained from Psychrobacter maritimus 3pS isolated from a Siberian cryopeg sample (Kolyma lowland). The following structure of the tetrasaccharide repeating unit of the polysaccharide was established by sugar analysis along with (1)H and (13)C NMR spectroscopy: â2)-α-L-Rhap-(1â4)-α-D-GalpNAcA-(1â3)-α-D-QuipNAc4NHb-(1â3)-ß-D-QuipNAc4NHb-(1â where D-GalNAcA indicates 2-acetamido-2-deoxy-D-galacturonic acid and d-QuiNAc4NHb indicates 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutanoyl]amino-D-glucose.
Subject(s)
Hexosamines/chemistry , Polysaccharides, Bacterial/chemistry , Polysaccharides, Bacterial/isolation & purification , Psychrobacter/chemistry , Carbohydrate Sequence , Hydrogen-Ion Concentration , Magnetic Resonance Spectroscopy , Molecular Sequence DataABSTRACT
Psychrotrophic bacteria of the genus Psychrobacter have not been studied in respect to lipopolysaccharide structure. In this work, we determined the structure of the O-specific polysaccharide of the lipopolysaccharide of Psychrobacter muricolla 2pS(T) isolated from overcooled (-9°C) water brines within permafrost. The polysaccharide was found to be acidic due to the presence of an amide of 2-acetamido-2-deoxy-l-guluronic acid with glycine (l-GulNAcA6Gly), which has not been hitherto found in nature. The following structure of the disaccharide repeating unit of the polysaccharide was established using composition analysis along with 1D and 2D (1)H and (13)C NMR spectroscopy: â4)-α-l-GulpNAcA6Gly-(1â3)-ß-d-GlcpNAc-(1â
