ABSTRACT
The aim of the study carried out on ten young (10-week old) pigs of the native Polish Large White breed experimentally infected with a low dose of 300 invasive muscle larvae (ML) of Trichinella spiralis was intravital detection of trichinellosis using the E-S ELISA test, determination of a variation level of IgG antibodies against excretory-secretory (E-S) antigens of T. spiralis muscle larvae and finally, describing the intensity of T. spiralis larvae infection in selected muscles. The pig sera were collected at 7 and 9 days prior to the experimental infection with T. spiralis and at 9, 14, 20, 23, 25, 27, 30, 33, 37, 41, 46 days post-infection (d.p.i.). The anti-T. spiralis IgG antibodies were detected by a commercial E-S ELISA test (PrioCHECK Trichinella Ab). Average intensity of the T. spiralis infection in the examined muscles of pigs ranged from 1.52 up to 43.09 larvae/g. The studies revealed that the E-S antigen in the ELISA test did not show cross-reaction with the sera of pigs infected with Oesophagostomum spp. The ELISA assay did not recognize trichinellosis in pigs until 27 days after the T. spiralis infection. The anti-T. spiralis IgG antibodies were first detected on day 30 post-infection. A statistically significant increase of IgG antibodies against T. spiralis ML E-S antigens was first observed between days 27-30 (p<0.01) post-infection, and a further significant rise in the antibody level occurred between days 27 and 33 (p<0.01); 30 and 33 (p<0.01); 33 and 37 (p<0.05) following infection.
Subject(s)
Enzyme-Linked Immunosorbent Assay/veterinary , Swine Diseases/diagnosis , Trichinellosis/veterinary , Animals , Antibodies, Helminth , Antigens, Helminth/blood , Enzyme-Linked Immunosorbent Assay/methods , Feces/parasitology , Helminth Proteins , Larva , Muscle, Skeletal/parasitology , Swine , Swine Diseases/parasitology , Trichinella spiralis , Trichinellosis/diagnosisABSTRACT
The purpose of this study was to assess the efficacy of the photodynamic diagnosis (PDD) method in the diagnosis of lesions of the uterine cervix, as well as to establish its place and efficacy among currently available diagnostic techniques. In the case of lesions located on the cervix, the projected aim of the study was intended to be realised by performing a detailed comparative analysis of the examination results of the following: PDD, cytology, colposcopy and testing for the presence of viruses, as compared with histological evaluation of performed biopsies.
Subject(s)
Photochemotherapy/methods , Uterine Cervical Dysplasia/diagnosis , Uterine Cervical Neoplasms/diagnosis , Adult , Aged , Biopsy , Cervix Uteri/pathology , Colposcopy , Cytological Techniques , DNA Probes , DNA, Viral , Female , Humans , Middle Aged , Treatment Outcome , Uterine Cervical Diseases/diagnosisABSTRACT
The purpose of this study was to evaluate the efficiency of photodynamic diagnostics (PDD), a method used in the diagnosis of vulvar lesions, and to determine its position and efficacy among diagnostic techniques generally used up to now. The projected purpose of the study in cases of vulvar lesions was realised by performing a detailed comparative analysis of sensitivity, specificity and diagnostic efficacy of PDD and vulvoscopy, as compared with histological evaluation of excised lesions.
