Subject(s)
Bacterial Infections/epidemiology , Respiratory Tract Infections/etiology , Virus Diseases/epidemiology , Adenoviridae/isolation & purification , Bacteria/isolation & purification , Child , Herpes Simplex/epidemiology , Humans , Nasopharynx/microbiology , Orthomyxoviridae/isolation & purification , Orthomyxoviridae Infections/epidemiology , Paramyxoviridae Infections/epidemiology , Respiratory Tract Infections/immunology , Respiratory Tract Infections/microbiology , Rhinovirus/isolation & purification , Rubella virus/isolation & purification , Thailand , Tropical ClimateSubject(s)
Penicillins/administration & dosage , Respiratory Tract Infections/drug therapy , Tetracycline/administration & dosage , Administration, Oral , Bacteria/isolation & purification , Child , Child, Preschool , Clinical Trials as Topic , Evaluation Studies as Topic , Female , Haemophilus Infections/drug therapy , Haemophilus influenzae , Humans , Infant , Male , Nasopharynx/microbiology , Placebos , Pneumococcal Infections/drug therapy , Respiratory Tract Infections/complications , Staphylococcal Infections/drug therapy , Thailand , Viruses/isolation & purificationSubject(s)
Occlusive Dressings , Oxytetracycline/therapeutic use , Wound Infection/prevention & control , Aerosols/therapeutic use , Animals , Bacitracin/therapeutic use , Neomycin/therapeutic use , Penicillin Resistance , Penicillins/therapeutic use , Polymyxins/therapeutic use , Rabbits , Soil Microbiology , Wounds and Injuries/microbiologySubject(s)
Soil Microbiology , Wound Infection/microbiology , Animals , Female , Male , Rabbits , VietnamABSTRACT
Experimental clostridial myonecrosis closely resembling gas gangrene was produced in goats by the intramuscular injection of washed clostridia suspended in adrenalin. The preparation provides a model for the study of clostridial myonecrosis that is relatively isolated from preceding trauma. A new test for the demonstration of clostridial exotoxins, the hemolysin indicator plate test, was described. This test gave reliable results within 6 hr, and only minute quantities of test material were required. Application of this test to would exudates of goats with experimental clostridial myonecrosis demonstrated the presence of the gamma toxin of Clostridium novyi, alpha toxin of C. septicum, and the alpha toxin of C. perfringens in exudates from animals injected with washed Clostridium bacilli suspended in adrenalin. Exudates from goats injected with C. perfringens were lethal for mice and produced necrosis of guinea pig skin typical of C. perfringens. The convincing demonstration of clostridial exotoxins formed in vivo in this experimental model could be the basis for definition of clostridial exotoxin actions which have evaded study in the past.
