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1.
Bioorg Khim ; 36(4): 437-81, 2010.
Article in Russian | MEDLINE | ID: mdl-20823915

ABSTRACT

The use of the reaction of azide and alkyne cycloaddition for the synthesis of nucleic acid conjugates and DNA oligomer analogues is considered. The data on chemical and enzymatic techniques of azides and alkynes introduction into DNA are summarized.


Subject(s)
Alkynes/chemistry , Azides/chemistry , DNA/chemistry
2.
Bioorg Khim ; 13(5): 623-7, 1987 May.
Article in Russian | MEDLINE | ID: mdl-3040012

ABSTRACT

Treatment of molecular crystals of the bovine cytochrome oxidase and the cytochrome oxidase-cytochrome c complex with thermally activated tritium leads to highly labelled cytochrome oxidase preparations. HPLC separation of its subunits and measurements of radioactivity of each polypeptide allow to determine the shielding of cytochrome oxidase surface sites by cytochrome c in the complex.


Subject(s)
Cytochrome c Group/metabolism , Electron Transport Complex IV/metabolism , Tritium , Animals , Cattle , Chromatography, High Pressure Liquid , Models, Biological , Myocardium/enzymology , Peptides/metabolism , Substrate Specificity
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