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1.
J Cancer Res Clin Oncol ; 128(12): 650-8, 2002 Dec.
Article in English | MEDLINE | ID: mdl-12474051

ABSTRACT

PURPOSE: The aim of this study was to determine TP53 and NM23-H1 immunoreactivity, DNA ploidy, and S-phase fraction (SPF) in a series of 160 patients undergoing resective surgery for primary operable colorectal cancer (CRC) and to establish whether these alterations have any clinical value in predicting CRC patients' prognosis. METHODS: TP53 and NM23-H1 expressions were evaluated on paraffin-embedded tissue by immunohistochemistry and DNA-ploidy and SPF on frozen tissue by flow-cytometric analysis. RESULTS: The median follow-up time in our study group was 71 months (range 34-115 months). P53 protein expression was associated with distal tumors (P<0.05) and DNA aneuploid tumors (P<0.05) tumors. DNA-aneuploidy was associated with distal tumors (P<0.01), histological grade (G3) (P<0.05), advanced Dukes' stage (C and D) (P<0.01), lymph node metastases (P<0.01) and high SPF (>18.3%) (P<0.01). The major significant predictors for both disease relapse and death were advanced Dukes' stage, DNA-aneuploidy, and high SPF, while lymphohematic invasion was the only independent factor for relapse and non-curative resection for death. CONCLUSIONS: Our results indicate that DNA aneuploidy and high SPF are associated in CRC with a poor clinical 5-year outcome, while in contrast the prognostic role of TP53 and NM23-H1 expression is still to be clarified.


Subject(s)
Colorectal Neoplasms/genetics , DNA, Neoplasm/genetics , Monomeric GTP-Binding Proteins/genetics , Nucleoside-Diphosphate Kinase , Ploidies , Transcription Factors/genetics , Tumor Suppressor Protein p53/genetics , Adenocarcinoma/genetics , Adenocarcinoma/mortality , Adenocarcinoma/pathology , Adenocarcinoma/surgery , Adenocarcinoma, Mucinous/genetics , Adenocarcinoma, Mucinous/mortality , Adenocarcinoma, Mucinous/pathology , Adenocarcinoma, Mucinous/surgery , Biomarkers, Tumor/analysis , Cell Division , Colon/pathology , Colorectal Neoplasms/mortality , Colorectal Neoplasms/pathology , Colorectal Neoplasms/surgery , Disease-Free Survival , Humans , Immunohistochemistry , Lymph Nodes/pathology , NM23 Nucleoside Diphosphate Kinases , Neoplasm Staging , Predictive Value of Tests , S Phase , Survival Analysis , Time Factors , Treatment Outcome
2.
Minerva Chir ; 54(4): 239-44, 1999 Apr.
Article in Italian | MEDLINE | ID: mdl-10380522

ABSTRACT

BACKGROUND AND AIMS: The increasingly frequent use of mammography for the early diagnosis of breast cancer and the consequent identification of mammary lesions at a preclinical stage raises the fundamental problem of the differential diagnosis between non-suspected non-palpable lesions (NPL) which can therefore be monitored over time and suspected NPL or definite carcinoma requiring histological confirmation and surgical biopsy. The diagnostic accuracy of mammography alone is not sufficiently high to differentiate benign lesions from malignant or strongly suspected ones. The use of surgical biopsy in the event of suspected NPL could be significantly reduced by the use of stereotaxic cytology which would improve the diagnostic accuracy of mammography. METHODS: The study refers to 72 suspected NPL undergoing surgical biopsy after having performed stereotaxic cytology on a sample taken with a dedicated mammographic device (Mammotest-TRC). RESULTS: The rate of inadequate samples for correct cytological evaluation was 16.1%. Of the 72 NPL undergoing surgical biopsy, 40 (55.5%) were found to be carcinomas and 32 (44.5%) were benign lesions. The sensitivities of mammography alone and cytology alone in identifying infraclinical breast carcinoma were respectively 0.85 and 0.95. If the results of the two methods were evaluated together, the level of sensitivity was 0.98. CONCLUSIONS: The use of stereotaxic cytology enables a marked improvement to be achieved in the diagnostic accuracy of mammography for the identification of suspected NPL to undergo surgical biopsy, notably reducing the cost of biopsy (number of benign lesions for each carcinoma diagnosed) and consequent discomfort for patients.


Subject(s)
Breast Diseases/diagnosis , Palpation , Aged , Biopsy , Breast Neoplasms/diagnosis , Carcinoma/diagnosis , Cytodiagnosis/methods , Cytodiagnosis/statistics & numerical data , Diagnosis, Differential , Female , Humans , Mammography , Middle Aged
3.
Anticancer Res ; 16(4B): 2257-67, 1996.
Article in English | MEDLINE | ID: mdl-8694553

ABSTRACT

The traditional prognostic factors, including stage of disease and tumour grade, have shown a limited prognostic significance and an inability to predict clinical response to specific treatment in patients with laryngeal squamous-cell carcinoma. More recent data suggest that cell kinetics indices, DNA-ploidy, lysosomal cysteine proteinase expression and genetic changes of both tumour suppressor genes and protooncogenes may be considered as reliable and reproducible indicators of biological aggressiveness in these patients. Moreover, the frequency of different genetic alterations suggests that several pathways are involved in the genesis of these neoplasias and, in particular, it is very probable that p-53 expression and PCNA indices (increased in normal mucosa and preinvasive lesions) may constitute more important biomarkers for the early steps of laryngeal carcinogenesis.


Subject(s)
Laryngeal Neoplasms/pathology , Cell Division , Chromosomes, Human, Pair 11 , DNA, Neoplasm/analysis , Flow Cytometry , Genes, p53 , Genes, ras , Humans , Laryngeal Neoplasms/genetics
4.
Cell Prolif ; 29(3): 153-62, 1996 Mar.
Article in English | MEDLINE | ID: mdl-8652744

ABSTRACT

In this study, 32 pleomorphic adenomas (PAs) and seven adenoid cystic carcinomas (ACCs) were analysed for the evaluation of proliferating cell nuclear antigen (PCNA) indices and flow cytometric variables. Our aim was to assess any possible relationship between these parameters and the clinico-pathological variables and to clarify their histogenesis and reasons for their biological differences. The tumours were divided into three groups, mainly epithelial (E), myxoid (M) and chondroid (C); PCNA labelling index (LI) and weighted mean index (WI) and the WI/LI ratio were analysed in the predominant components; a single PCNA index, weighted by the percentage of each component, was also calculated. Only WI/LI was found to be significantly different in the three components, while PCNA single index did not show either significant differences by sex, age, site and size, or any correlation with the S phase fraction. A significant difference was found between PAs and ACCs by site (P < 0.01) and DNA ploidy (P < 0.05); furthermore, all PCNA indices (single index) were significantly lower in PAs than in ACCs.


Subject(s)
Adenoma, Pleomorphic/physiopathology , Carcinoma, Adenoid Cystic/physiopathology , Salivary Gland Neoplasms/physiopathology , Adenoma, Pleomorphic/genetics , Adolescent , Adult , Aged , Aneuploidy , Carcinoma, Adenoid Cystic/genetics , Cell Division/physiology , DNA, Neoplasm/analysis , Female , Flow Cytometry , Humans , Immunohistochemistry , Male , Middle Aged , Ploidies , Proliferating Cell Nuclear Antigen/analysis , S Phase/physiology , Salivary Gland Neoplasms/genetics
5.
Cancer Res ; 55(24): 6103-8, 1995 Dec 15.
Article in English | MEDLINE | ID: mdl-8521400

ABSTRACT

A consecutive series of 99 untreated patients undergoing radical surgical resection for stage I-IV laryngeal carcinomas has been studied prospectively. Our purpose was to analyze the predictive relevance of proliferative variables studied [proliferating cell nuclear antigen (PCNA) expression, volume-corrected mitotic (M/V) index, and S-phase fraction (SPF)] on clinical outcome in relation to DNA ploidy and clinicopathological features. All of the patients were followed up for a median of 32 months (range, 5-58 months). A weak, but significant, positive correlation was found between M/V and PCNA indices (except the PCNA weighted mean index:labeling index ratio) or these indices and SPF. At univariate analysis, node positivity (P < 0.05), poor histological grade (P < 0.01), DNA aneuploidy (P < 0.01), a high SPF (P < 0.01), and a high M/V index (P < 0.05) proved to be related significantly to quicker relapse, whereas T4 (P < 0.05), subglottic site (P < 0.05), DNA aneuploidy (P < 0.01) and a high SPF (P < 0.01) were related significantly to shorter overall survival. With multivariate analysis, a high SPF (> 12.1%) and histological grade (G3) were related to the risk of relapse (relative risk, 8.65 and 5.45, respectively), whereas only a high SPF was related independently to the risk of death (relative risk, 7.30). Our study has identified SPF, in addition to histological grade, as an important biological indicator in laryngeal carcinomas.


Subject(s)
Carcinoma, Squamous Cell/pathology , Cell Division , DNA, Neoplasm/metabolism , Laryngeal Neoplasms/pathology , Aged , Carcinoma, Squamous Cell/diagnosis , Female , Humans , Laryngeal Neoplasms/diagnosis , Male , Middle Aged , Multivariate Analysis , Ploidies , Prognosis , Proliferating Cell Nuclear Antigen/metabolism , S Phase , Survival Analysis
6.
Anal Quant Cytol Histol ; 16(5): 365-74, 1994 Oct.
Article in English | MEDLINE | ID: mdl-7530959

ABSTRACT

A series of 71 patients undergoing surgery for primary breast carcinoma was prospectively studied in order to evaluate the relative weight for four biologic factors (intermediate filament vimentin expression, proliferating cell nuclear antigen [PCNA], flow cytometric DNA ploidy and S-phase fraction) and of several clinicopathologic and biologic features in predicting clinical outcome (disease-free interval). In univariate statistical analysis, positivity of axillary nodes, high number of mitoses, high nuclear grade, high histologic grade, positivity of vimentin, high flow cytometric S-phase fraction (FCM-S) value, high PCNA and high silver-stained nuclear organizer regions scores were significantly related to risk of relapse. In multivariate analysis (Cox's logistic regression) only histologic grade (3) and high FCM-S values (> 10.7) were independently related to risk of relapse, with hazard ratios of 9.84 and 7.98, respectively. The results of our preliminary, prospective study suggest that FCM-S, in addition to morphologic criteria (histologic grade), may be an important biologic indicator in determining breast cancer patients' prognosis.


Subject(s)
Breast Neoplasms/chemistry , Flow Cytometry , Proliferating Cell Nuclear Antigen/analysis , Vimentin/analysis , Analysis of Variance , DNA/analysis , Humans , Keratins/analysis , Nucleolus Organizer Region/ultrastructure , Prospective Studies , Receptors, Progesterone/analysis , Silver Staining
7.
Pathologica ; 86(3): 227-33, 1994 Jun.
Article in English | MEDLINE | ID: mdl-7808790

ABSTRACT

In this study both the incidence and pattern of p53 over-expression in various histological subtypes of a series of 36 cases of renal cell Grawitz carcinoma, partially studied in a previous paper, were analyzed. This series consisted of these histologic subtypes: clear cell non papillary (18 cases), clear cell papillary (2 cases), granular cell (5 cases), mixed (clear and granular cell) (9 cases) and spindle cell (2 cases). At present, our aim was, firstly, to see which were the best technical conditions for detection of p53 in the available paraffin-embedded tumor specimens, using several antibodies, specific for various epitopes; secondly, to investigate if some relation might exist between this expression and the histological features of these tumors. Twenty-five per cent (9/36 cases) resulted p53 immunoreactive, the highest percentage being represented in the papillary clear and granular cell carcinomas; low expression was detected in 11 cases (30%) and no reactivity in 16 cases (44%). Neither technical or dilution modifications proved to transform these latter results; however, detection was maximal using the CM-1 polyclonal rabbit antiserum. Thus, in RCC, expression of p53, analyzed in the light of the cytogenetic characterization through a literature review, resulted at low frequency. This finding means that mutation of the p53 gene are not frequent in the neoplastic transformation in RCC. Nevertheless, in spite of the small number of cases and of the short follow-up period of this study, detection of p53 positivity in tumors with either high grade and stage or high proliferative activity could suggest that p53 mutations lead to tumors of a more aggressive type.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Carcinoma, Renal Cell/genetics , Gene Expression Regulation, Neoplastic/genetics , Kidney Neoplasms/genetics , Tumor Suppressor Protein p53/biosynthesis , Aged , Female , Humans , Immunohistochemistry , Male , Tumor Suppressor Protein p53/genetics
8.
Int J Oncol ; 5(3): 495-500, 1994 Sep.
Article in English | MEDLINE | ID: mdl-21559604

ABSTRACT

A series of 71 patients undergoing radical surgical resection for stage III and IV laryngeal carcinoma (LC) consecutively diagnosed was prospectively studied in order to evaluate the relative weight of p53 expression in predicting clinical outcome, All the patients taking part in this study were followed up for a median of 18 months (range: 6-41 months). Positive staining for p53 protein was detected in 44 of 71 (62%) of these tumors on paraffin-embedded tissue, even in dysplastic areas. Among the clinico-pathological and biological parameters analyzed, only flow-cytometric S-phase (FCM-S) Values of turners showed a significant relationship to p53 immunostaining (p=0.01). With Kaplan-Meier estimation, in multivariate analysis only high FCM-S (>15.1) was independently related to risk of relapse (RR=5.82), while both FCM-S and site (subglottis) were related to risk of death (RR=6.83 and RR=14.3, respectively). These findings indicate that p53 immunoreactivity, though of no utility as a prognostic indicator, probably plays a role in the early stages of LC tumorigenesis.

9.
Oncol Rep ; 1(6): 1063-8, 1994 Nov.
Article in English | MEDLINE | ID: mdl-21607491

ABSTRACT

A malignant myoepithelioma, mainly intraductally growing, of the breast was studied, emphasizing the immunohistological features of such tumors, in comparison with the more common, architecturally similar, infiltrating ductal carcinoma with intraductal component. Immunohistochemically, cell population of the tumor studied expressed intense reaction to alpha-SM-actin, cytokeratin 14, vimentin, S-100 protein and collagen IV antibodies, confirming myoepithelial differentiation. Since myoepitheliomas of the breast are very rare, complete knowledge of these neoplasms has not been attained. Nevertheless, histogenetic investigations may be useful in differentiating this type of tumor, from the above one, which is known to be characterized by severe clinical and prognostic implications.

10.
Pathologica ; 85(1100): 607-35, 1993.
Article in Italian | MEDLINE | ID: mdl-8170711

ABSTRACT

Microglandular hyperplasia is a lesion of the uterine endocervical mucosa, often associated with a story of contraceptive drugs or pregnancy. It was frequently confused with adenocarcinoma or premalignant glandular changes until its benign nature was recognized. In order to ascertain clinical presentation, hormonal or gestational status, histologic patterns with their variants and cytologic counter-part, we collected and reviewed 28 cases of this condition over a period of a decade. The age ranged from 23 to 54, with a mean of 37.2 years. 11 patients had been receiving oral contraceptives and 5 were pregnant. All samples came from uterine cervix. Cervical smears were available from 17 women. Four histologic patterns were identified, including glandular, reticular, trabecular and solid. Cytologic features varied from aspecific, inflammatory changes of the columnar endocervical cells to more particular findings, including clear cells, strips, sheets, papillae, rosettes and corolla-like aggregates. Moreover, differential diagnostic criteria from cervical neoplasms and various pseudoneoplastic conditions of the cervical glandular epithelium are discussed. An extensive review of the literature is also presented.


Subject(s)
Cervix Uteri/pathology , Adenocarcinoma/diagnosis , Adult , Cervix Uteri/drug effects , Contraceptives, Oral, Hormonal/adverse effects , Diagnosis, Differential , Female , Humans , Hyperplasia , Menopause , Middle Aged , Mucous Membrane/pathology , Polyps/chemically induced , Polyps/pathology , Precancerous Conditions/chemically induced , Precancerous Conditions/pathology , Pregnancy , Pregnancy Complications/pathology , Retrospective Studies , Uterine Cervical Diseases/chemically induced , Uterine Cervical Diseases/pathology , Uterine Cervical Neoplasms/chemically induced , Uterine Cervical Neoplasms/diagnosis , Uterine Cervical Neoplasms/pathology , Vaginal Smears
11.
In Vivo ; 7(6B): 601-5, 1993.
Article in English | MEDLINE | ID: mdl-7514895

ABSTRACT

A series of 71 patients undergoing surgery for primary breast carcinoma was prospectively studied in order to verify the relationships between clinical and pathological variables, proliferative indexes and DNA-ploidy. Significant correlations between proliferative indexes were found; conversely, DNA-ploidy showed no correlation with either PCNA/cyclin or AgNORs, but only with flow cytometry S-phase fraction (FCM-S) and IF-vimentin. In the 42 patients with a mean follow-up of 42 months, disease-free rate was best predicted by nuclear grade (p < 0.001), histological grade (p < 0.001), mitoses (p 0.001), IF-vimentin (p 0.002), FCM-S (p 0.002), PCNA (p 0.005) and Ag-NORs (p 0.007).


Subject(s)
Biomarkers, Tumor , Breast Neoplasms/diagnosis , Heat-Shock Proteins , Adult , Aged , DNA, Neoplasm/analysis , Humans , Keratins/analysis , Middle Aged , Nuclear Proteins/analysis , Nucleolus Organizer Region/ultrastructure , Phosphoproteins/analysis , Prognosis , Proliferating Cell Nuclear Antigen , Prospective Studies , Receptors, Estrogen/analysis , Receptors, Progesterone/analysis , S Phase , Vimentin/analysis
12.
Pathologica ; 85(1097): 343-52, 1993.
Article in English | MEDLINE | ID: mdl-7694227

ABSTRACT

Since morphological features of renal cell carcinomas may not always predict their clinical behaviour, other biological parameters are more and more searched for, as prognostic indicators. Among those, the Authors evaluate the cytokeratin-vimentin intermediate-sized filaments co-expression in a series of 32 renal carcinomas and analyze this feature in correlation with both traditional criteria and proliferative activity of tumor cells, studied by means of the proliferation cellular nuclear antigen (PCNA) expression as well as of the nucleolar organizer regions (NORs) count, within tumor cells. From this study, nuclear grade stands out as the best parameter which, furthermore, shows a significant relationship with the cytokeratin-vimentin co-expression and the mean NORs count in tumor cell populations. Further investigations about relationships between these parameters and survival of the patients, who, at present, are almost all alive, will be performed afterwards.


Subject(s)
Carcinoma, Renal Cell/pathology , Kidney Neoplasms/pathology , Adult , Aged , Antigens, Neoplasm/analysis , Carcinoma, Renal Cell/chemistry , Cell Division , Female , Follow-Up Studies , Humans , Immunohistochemistry , Keratins/analysis , Kidney Neoplasms/chemistry , Male , Middle Aged , Nuclear Proteins/analysis , Nucleolus Organizer Region/pathology , Proliferating Cell Nuclear Antigen , Vimentin/analysis
13.
Minerva Chir ; 45(23-24): 1439-45, 1990 Dec.
Article in Italian | MEDLINE | ID: mdl-1965017

ABSTRACT

There are still marked differences in the current indications for breast screening proposed by the various international school of oncology. Epidemiological data to the effect that breast screening in asymptomatic women aged over 50 reduces the death rate due to breast cancer now appears to be widely accepted, but an analogous finding for women aged between 40-49 has not yet been confirmed. Following a brief analysis of the most important breast screening programmes carried out to date, the Authors report the preliminary results regarding the identification and biopsy of non-palpable breast lesions during the course of a screening programme in 1986 by the Dept. of Cancer Surgery. Of a total of 1128 breast scans in asymptomatic patients aged between 40 and 73, 24 suspect (1.9%) non-palpable lesions were found of which 5 (20.8%) proved to be carcinomas.


Subject(s)
Adenofibroma/prevention & control , Breast Neoplasms/prevention & control , Carcinoma, Intraductal, Noninfiltrating/prevention & control , Mammography , Papilloma/prevention & control , Adenofibroma/diagnosis , Adenofibroma/diagnostic imaging , Adult , Age Factors , Aged , Breast Neoplasms/diagnosis , Breast Neoplasms/diagnostic imaging , Carcinoma, Intraductal, Noninfiltrating/diagnosis , Carcinoma, Intraductal, Noninfiltrating/diagnostic imaging , Diagnosis, Differential , Female , Fibrocystic Breast Disease/diagnosis , Humans , Middle Aged , Papilloma/diagnosis , Papilloma/diagnostic imaging , Prognosis , Time Factors
14.
Int J Biol Markers ; 4(2): 95-102, 1989.
Article in English | MEDLINE | ID: mdl-2549126

ABSTRACT

A series of 65 cases of different histological types of breast carcinoma was investigated for the immunohistochemical location of the estrogen receptor-related, 29 kD phosphoprotein using the ER-D5 monoclonal antibody. The ER-D5 response is heterogeneous in relation to some therapeutic limitations and is correlated with histopathological features of the tumors and survival. The main parameters for evaluation of breast cancers are reviewed, both those that are statistically correlated and those that are not apparently always correlated but are known to have considerable biological meaning, such as the ER-status of tumors.


Subject(s)
Biomarkers, Tumor/analysis , Breast Neoplasms/pathology , Phosphoproteins/analysis , Receptors, Estrogen/analysis , Adenocarcinoma/analysis , Adenocarcinoma/pathology , Adenocarcinoma, Mucinous/analysis , Adenocarcinoma, Mucinous/pathology , Adult , Aged , Aged, 80 and over , Breast Neoplasms/analysis , Carcinoma, Intraductal, Noninfiltrating/analysis , Carcinoma, Intraductal, Noninfiltrating/pathology , Carcinoma, Papillary/analysis , Carcinoma, Papillary/pathology , Humans , Middle Aged , Neoplasm Invasiveness
18.
Int J Biol Markers ; 2(1): 1-8, 1987.
Article in English | MEDLINE | ID: mdl-2448399

ABSTRACT

A series of 20 cases of pleomorphic adenoma and 19 cases of adenoid-cystic carcinoma of the salivary glands, and one case in the mammary location, were investigated regarding immunohistochemical reactivity for Tissue Polypeptide Antigen (TPA), Pre-Keratins, Vimentin, S-100 Protein, and their arrangement pattern of fibronectin. As a whole, the results support the hypothesis of morpho-structural and mainly, onto-histogenetic similarities between these tumours, but they also underline the need for great care in outlining their morpho-functional features, in relation to their different prognoses.


Subject(s)
Adenoma, Pleomorphic/analysis , Carcinoma, Adenoid Cystic/analysis , Parotid Neoplasms/analysis , Peptides/analysis , Proteins/analysis , Fibronectins/analysis , Glycoproteins/analysis , Humans , Immunohistochemistry , Keratins/analysis , Protein Precursors/analysis , Protein S , Tissue Polypeptide Antigen , Vimentin/analysis
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