[Immunocompetent cells in organs of local and systemic immunity in intact and sensitized guinea pigs upon specific intranasal immunotherapy]. / Immunokompetentnye kletki v organakh lokal'nykh i sistemnogo immuniteta u intaktnykh i sensibilizirovannykh morskikh svinok pri spetsificheskoi intranazal'noi immunoterapii.

The normal levels of some lymphocyte populations and subpopulations in the lungs and lymph nodes draining the respiratory tract have been determined. The influence of systemic delayed hypersensitivity (DH) to staphylococci and the multiple introduction of staphylococcal antigen into intact and sensitized guinea pigs on the distribution of lymphocyte subpopulations in the central, peripheral and respiratory lymphoid organs has been studied. Systemic DH has been found to affect the level of immunocytes in the thymus and peripheral lymphoid organs; the local introduction of the allergen and the intranasal immunotherapy of systemic DH produce both local (the lungs and their lymph nodes) and systemic effect.

[Effect of detergent and glycerin on the efficacy of nasal immunotherapy with allergens and on the level of the lymphocyte subpopulation in the organs of local immunity]. / Vliianie detergenta i glitserina na éffektivnost' nazal'noi immunoterapii allergenami i uroven' subpopuliatsii limfotsitov v organakh mestnogo immuniteta.

Experimental mixed allergy to staphylococcal antigens in guinea pigs was treated by the intranasal administration of a staphylococcal allergen with a surfactant or glycerin added. The treatment was found to produce a hyposensitizing effect with respect to immediate and delayed hypersensitivity. The addition of glycerine enhanced this effect. At the same time the level of T-lymphocytes in the lungs and the lymph nodes of the respiratory tract returned to normal. Detergent used at a concentration of 2% abolished the hyposensitizing effect of the allergen, stimulated T-lymphocytes in the lymph nodes of the respiratory tract and the lungs; the number of T-suppressors decreased.

[Lymphocyte subpopulations in sensitization and specific immunotherapy in an experiment. Lymphocyte subpopulations in sensitization to staphylococci, Artemisia pollen and their combinations]. / Subpopuliatsii limfotsitov pri sensibilizatsii i spetsificheskoi immunoterapii v éksperimente. Subpopuliatsii limfotsitov pri sensibilizatsii k stafilokokkam, pyl'tse polyni i ikh sochetanii.

The distribution of T- and B-lymphocytes in the body of guinea pigs was studied in different groups of the animals. As shown in this study, in delayed hypersensitivity to staphylococci the number of PE- and E-rosette-forming cells increased in the blood, the spleen, and the lymph nodes and decreased in the thymus; the number of EA- and EAC-rosette-forming cells decreased in the bone marrow and the spleen, the number of T gamma-suppressors decreased in the bone marrow and the distant lymph node. Immediate hypersensitivity to tarragon pollen induced the general increase of the content of T- and B-lymphocytes; the number of T gamma-cells decreased in the thymus, the bone marrow, and the lymph nodes and increased in the spleen. The characteristic features of combined microbial-pollen sensitization were the high content of B-cells in all lymphoid organs (except the thymus), a low level of T-lymphocytes in the blood and the peripheral lymphoid organs, the decreased number of T gamma-cells in most of the immunogenetic organs.

[Lymphocyte subpopulations in sensitization and specific immunotherapy in an experiment. Lymphocyte subpopulations in the specific immunotherapy of microbial allergy and subsequent heterologous pollen sensitization]. / Subpopuliatsii limfotsitov pri sensibilizatsii i spetsificheskoi immunoterapii v éksperimente. Subpopuliatsii limfotsitov pri spetsificheskoi immunoterapii mikrobnoi allergii i posleduiushchei geterologichnoi pyl'tsevoi sensibilizatsii.

The influence exerted by the specific immunotherapy (SIT) of delayed hypersensitivity (DH) to staphylococci and subsequent sensitization with tarragon pollen on the level of immunocompetent cells in the blood and lymphoid organs of guinea pigs was studied. On the whole, SIT normalized the characteristics of T- and B-lymphocytes, altered as the result of experimentally induced DH: the content of T-cells in the peripheral blood and the lymph nodes increased, while the number of B-cells in the blood and T gamma-suppressors increased. The subsequent heterologous sensitization with pollen abolished the effect of SIT, inducing the general decrease of the level of T gamma-lymphocytes and enhancing the number of T-lymphocytes in the lymph nodes.

[Formation of pollen-induced allergy in guinea pigs after specific immunotherapy with a bacterial allergen]. / Formirovanie pyl'tsevoi allergii u morskikh svinok posle spetsificheskoi immunoterapii bakterial'nym allergenom.

The investigation was aimed at elucidating the process of the development of allergy to exoallergens (e.g., to linear-leaf wormwood pollen) in the body after desensitization by specific immunotherapy (SIT) with heterologous (staphylococcal) allergen. The study revealed that in staphylococcal allergy the subsequent development of pollen sensitization occurred with greater intensity, and SIT was found to exert no influence on this process. For the first time pollen allergy was found to produce an unfavorable effect on the quality and effectiveness of SIT with heterologous staphylococcal allergen, this effect consisting in the acceleration and intensification of the process leading to the recovery of sensitization to staphylococci. The importance of measures for limiting contacts with heterologous allergens and their elimination during SIT is emphasized.

[Specific experimental therapy in sensitization to group A streptococci]. / Spetsificheskaia eksperimental'naia terapiia pri sensibilizatsii k streptokokku gruppy A.

A model of delayed hypersensitivity to Streptococcus hemolyticus, group A, were obtained in guinea pigs and rabbits. Studies of spontaneous changes of the immuno-allergic reactivity revealed that after a month of sensitization there developed delayed hypersensitivity only; according to the results of late skin tests it lasted not less than 6 months (the duration of investigation). The delayed hypersensitivity component began to manifest itself in 2 1/2 to 3 months and increased later on. Specific hyposensitization was performed with streptococcus allergens differing by physico-chemical conditions, i.e. the corpuscula allergen, the one that was lysed by ultrasonic waves, and Ando-Verzhikovsky's allergen. The latter had the most intensive hyposensitizing features. Specific hyposensitization was more demonstrative after the intracutaneous long-term course injections of threshold doses. Administration of subthreshold doses, as well as subcutaneous or intravenous injections of the allergen, was ineffective.