ABSTRACT
Kanamycinâ A is the major 2-deoxystreptamine (2DOS)-containing aminoglycoside antibiotic produced by Streptomyces kanamyceticus. The 2DOS moiety is linked with 6-amino-6-deoxy-d-glucose (6ADG) at O-4 and 3-amino-3-deoxy-d-glucose at O-6. Because the 6ADG moiety is derived from d-glucosamine (GlcN), deamination at C-2 and introduction of C-6-NH2 are required in the biosynthesis. A dehydrogenase, KanQ, and an aminotransferase, KanB, are presumed to be responsible for the introduction of C-6-NH2 , although the substrates have not been identified. Here, we examined the substrate specificity of KanQ to better understand the biosynthetic pathway. It was found that KanQ oxidized kanamycinâ C more efficiently than the 3''-deamino derivative. Furthermore, the substrate specificity of an oxygenase, KanJ, that is responsible for deamination at C-2 of the GlcN moiety was examined, and the crystal structure of KanJ was determined. It was found that C-6-NH2 is important for substrate recognition by KanJ. Thus, the modification of the GlcN moiety occurs after pseudo-trisaccharide formation, followed by the introduction of C-6-NH2 by KanQ/KanB and deamination at C-2 by KanJ.
Subject(s)
Anti-Bacterial Agents/metabolism , Kanamycin/biosynthesis , Polysaccharides/chemistry , Anti-Bacterial Agents/chemistry , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Glycosylation , Kanamycin/analogs & derivatives , Kinetics , Oxidoreductases/genetics , Oxidoreductases/metabolism , Streptomyces/enzymology , Substrate Specificity , Transaminases/genetics , Transaminases/metabolismABSTRACT
Fluvirucins are 14-membered macrolactam polyketides that show antifungal and antivirus activities. Fluvirucins have the ß-alanine starter unit at their polyketide skeletons. To understand the construction mechanism of the ß-alanine moiety in fluvirucin biosyntheses, we have identified the biosynthetic cluster of fluvirucin B2 produced from Actinomadura fulva subsp. indica ATCC 53714. The identified gene cluster contains three polyketide synthases, four characteristic ß-amino acid-carrying enzymes, one decarboxylase, and one amidohydrolase. We next investigated the activity of the adenylation enzyme FlvN, which is a key enzyme for the selective incorporation of a ß-amino acid substrate. FlvN showed strong preference for l-aspartate over other amino acids such as ß-alanine. Based on these results, we propose a biosynthetic pathway for fluvirucin B2.
Subject(s)
Actinobacteria/genetics , Anti-Infective Agents/metabolism , Deoxy Sugars/biosynthesis , Gene Expression Regulation, Bacterial , Genome, Bacterial , beta-Alanine/metabolism , Actinobacteria/enzymology , Adenosine Monophosphate/metabolism , Amidohydrolases/genetics , Amidohydrolases/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Carboxy-Lyases/genetics , Carboxy-Lyases/metabolism , Cloning, Molecular , Deoxy Sugars/genetics , Escherichia coli/genetics , Escherichia coli/metabolism , Gene Expression , Lactams , Molecular Sequence Annotation , Multigene Family , Polyketide Synthases/genetics , Polyketide Synthases/metabolism , Polyketides/metabolism , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Substrate SpecificityABSTRACT
To isolate a key polyketide biosynthetic intermediate for the 16-membered macrolide FD-891 (1), we inactivated two biosynthetic genes coding for post-polyketide synthase (PKS) modification enzymes: a methyltransferase (GfsG) and a cytochrome P450 (GfsF). Consequently, FD-892 (2), which lacks the epoxide moiety at C8-C9, the hydroxy group at C10, and the O-methyl group at O-25 of FD-891, was isolated from the gfsF/gfsG double-knockout mutant. In addition, 25-O-methyl-FD-892 (3) and 25-O-demethyl-FD-891 (4) were isolated from the gfsF and gfsG mutants, respectively. We also confirmed that GfsG efficiently catalyzes the methylation of 2 and 4 in vitro. Further, GfsF catalyzed the epoxidation of the double bond at C8-C9 of 2 and 3 and subsequent hydroxylation at C10, to afford 4 and 1, respectively. These results suggest that a parallel post-PKS modification mechanism is involved in FD-891 biosynthesis.
Subject(s)
Macrolides/metabolism , Polyketide Synthases/metabolism , Streptomyces/enzymology , Chromatography, High Pressure Liquid , Cytochrome P-450 Enzyme System/genetics , Cytochrome P-450 Enzyme System/metabolism , Epoxy Compounds/chemistry , Hydroxylation , Kinetics , Macrolides/chemistry , Methylation , Methyltransferases/metabolism , Multigene Family , Mutagenesis, Site-Directed , Polyketide Synthases/geneticsABSTRACT
Hitachimycin is a macrolactam antibiotic with (S)-ß-phenylalanine (ß-Phe) at the starter position of its polyketide skeleton. To understand the incorporation mechanism of ß-Phe and the modification mechanism of the unique polyketide skeleton, the biosynthetic gene cluster for hitachimycin in Streptomyces scabrisporus was identified by genome mining. The identified gene cluster contains a putative phenylalanine-2,3-aminomutase (PAM), five polyketide synthases, four ß-amino-acid-carrying enzymes, and a characteristic amidohydrolase. A hitA knockout mutant showed no hitachimycin production, but antibiotic production was restored by feeding with (S)-ß-Phe. We also confirmed the enzymatic activity of the HitA PAM. The results suggest that the identified gene cluster is responsible for the biosynthesis of hitachimycin. A plausible biosynthetic pathway for hitachimycin, including a unique polyketide skeletal transformation mechanism, is proposed.
Subject(s)
Genomics , Intramolecular Transferases/genetics , Intramolecular Transferases/metabolism , Multigene Family , Phenylalanine/metabolism , Genome, Bacterial/genetics , Polyenes/chemistry , Polyenes/metabolism , Stereoisomerism , Streptomyces/enzymology , Streptomyces/genetics , Streptomyces/metabolismABSTRACT
Butirosin is an aminoglycoside antibiotic consisting two epimers at C-3'' of ribostamycin/xylostasin with a unique 4-amino-2-hydroxybutyrate moiety at C-1 of the aminocyclitol 2-deoxystreptamine (2DOS). To date, most of the enzymes encoded in the biosynthetic gene cluster for butirosin, from the producing strain Bacillus circulans, have been characterized. A few unknown functional proteins, including nicotinamide adenine dinucleotide cofactor-dependent dehydrogenase/reductase (BtrE and BtrF), are supposed to be involved in the epimerization at C-3'' of butirosin B/ribostamycin but remain to be characterized. Herein, the conversion of ribostamycin to xylsostasin by BtrE and BtrF in the presence of NAD(+) and NADPH was demonstrated. BtrE oxidized the C-3'' of ribostamycin with NAD(+) to yield 3''-oxoribostamycin. BtrF then reduced the generated 3''-oxoribostamycin with NADPH to produce xylostasin. This reaction step was the last piece of butirosin biosynthesis to be described.
Subject(s)
Alcohol Oxidoreductases/metabolism , Bacterial Proteins/metabolism , Butirosin Sulfate/biosynthesis , Butirosin Sulfate/chemistry , Oxidoreductases/metabolism , Alcohol Oxidoreductases/chemistry , Bacillus/enzymology , Bacillus/genetics , Bacterial Proteins/chemistry , Molecular Structure , NAD/metabolism , NADP/metabolism , Oxidoreductases/chemistry , Ribostamycin/analogs & derivatives , Ribostamycin/metabolism , Substrate SpecificityABSTRACT
Chondroitin sulfate (CS) containing GlcA-GalNAc(4,6-SO(4)) (E unit) and CS containing GlcA(2SO(4))-GalNAc(6SO(4)) (D unit) have been implicated in various physiological functions. However, it has been poorly understood how the structure and contents of disulfated disaccharide units in CS contribute to these functions. We prepared CS libraries containing E unit or D unit in various proportions by in vitro enzymatic reactions using recombinant GalNAc 4-sulfate 6-O-sulfotransferase and uronosyl 2-O-sulfotransferase, and examined their inhibitory activity toward thrombin. The in vitro sulfated CSs containing disulfated disaccharide units showed concentration-dependent direct inhibition of thrombin when the proportion of E unit or D unit in the CSs was above 15-17%. The CSs containing both E unit and D unit exhibited higher inhibitory activity toward thrombin than the CSs containing either E unit or D unit alone, if the proportion of the total disulfated disaccharide units of these CSs was comparable. The thrombin-catalyzed degradation of fibrinogen, a physiological substrate for thrombin, was also inhibited by the CS containing both E unit and D unit. These observations indicate that the enzymatically prepared CS libraries containing various amounts of disulfated disaccharide units appear to be useful for elucidating the physiological function of disulfated disaccharide units in CS.
Subject(s)
Antithrombins/chemistry , Antithrombins/pharmacology , Chondroitin Sulfates/chemistry , Chondroitin Sulfates/pharmacology , Disaccharides/chemistry , Thrombin/antagonists & inhibitors , Animals , Antithrombins/metabolism , Chondroitin Sulfates/biosynthesis , Dose-Response Relationship, Drug , Factor X/antagonists & inhibitors , Fibrinogen/metabolism , Humans , Small Molecule Libraries/chemistry , Small Molecule Libraries/pharmacology , Sulfotransferases/isolation & purification , Sulfotransferases/metabolism , Thrombin/metabolismABSTRACT
BtrN encoded in the butirosin biosynthetic gene cluster possesses a CXXXCXXC motif conserved within the radical S-adenosyl methionine (SAM) superfamily. Its gene disruption in the butirosin producer Bacillus circulans caused the interruption of the biosynthetic pathway between 2-deoxy-scyllo-inosamine (DOIA) and 2-deoxystreptamine (DOS). Further, in vitro assay of the overexpressed enzyme revealed that BtrN catalyzed the oxidation of DOIA under the strictly anaerobic conditions along with consumption of an equimolar amount of SAM to produce 5'-deoxyadenosine, methionine, and 3-amino-2,3-dideoxy-scyllo-inosose (amino-DOI). Kinetic analysis showed substrate inhibition by DOIA but not by SAM, which suggests that the reaction is the Ordered Bi Ter mechanism and that SAM is the first substrate and DOIA is the second. The BtrN reaction with [3-2H]DOIA generated nonlabeled, monodeuterated and dideuterated 5'-deoxyadenosines, while no deuterium was incorporated by incubation of nonlabeled DOIA in the deuterium oxide buffer. These results indicated that the hydrogen atom at C-3 of DOIA was directly transferred to 5'-deoxyadenosine to give the radical intermediate of DOIA. Generation of nonlabeled and dideuterated 5'-deoxyadenosines proved the reversibility of the hydrogen abstraction step. The present study suggests that BtrN is an unusual radical SAM dehydrogenase catalyzing the oxidation of the hydroxyl group by a radical mechanism. This is the first report of the mechanistic study on the oxidation of a hydroxyl group by a radical SAM enzyme.
Subject(s)
Bacillus/metabolism , Butirosin Sulfate/biosynthesis , Oxidoreductases/metabolism , S-Adenosylmethionine/metabolism , Amino Acid Motifs , Anti-Bacterial Agents/biosynthesis , Bacillus/enzymology , Bacillus/genetics , Hexosamines/metabolism , Kinetics , Mutagenesis, Insertional , Oxidoreductases/geneticsABSTRACT
Butirosin produced by Bacillus circulans is among the clinically important 2-deoxystreptamine containing aminoglycoside antibiotics and its unique structure is found in (S)-4-amino-2-hydroxyburyric acid substituted at C-1 of 2-deoxystreptamine. Recently, the key part of the butirosin biosynthetic gene cluster has been identified from Bacillus circulans SANK 72073, however the whole gene for the biosynthesis awaited for identification. In the present study, we undertook extended analysis of the butirosin biosynthetic gene cluster and found nine additional open reading flames (ORFs), btrQ, btrR1, btrR2, btrT, btrU, btrV, btrW, btrX and orf1 in the cluster. In addition, we constructed disruption mutants of btrR1 and btrP-V, and found that the btr genes (ca. 24Kb) between btrR1 and btrP-V are at least required for the butirosin biosynthesis.