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2.
Front Med (Lausanne) ; 7: 492, 2020.
Article in English | MEDLINE | ID: mdl-33015087

ABSTRACT

Hailey-Hailey disease (HHD) is genetic skin disorder characterized by repeated and exacerbated skin lesions in friction regions. ATP2C1, encoding SPCA1, was demonstrated to be the responsible gene for HHD pathogenesis. However, for some cases, no ATP2C1 mutation could be determined by standard Sanger sequencing, thereby obscuring the cause and diagnosis of HHD. In this study, we investigated the possibility that HHD is caused by complex ATP2C1 defects using multiplex ligation-dependent probe amplification (MLPA) analysis for 10 of 50 cases in our institute without ATP2C1 mutations. In one female Japanese patient and her father, who also show HHD, MLPA followed by polymerase chain reaction (PCR) analyses revealed a novel duplication of exons 8-16 of ATP2C1. The duplication was predicted to add 20,615 base pairs, 882 nt, and 294-amino-acid residues to the genome, mRNA and SPCA1 protein, respectively. By reverse transcriptase-PCR using patient skin RNA, we could confirm that a larger transcript was produced and we found that the abundance of the normal transcript was clearly reduced in the patient. Putative structures of wild-type and duplicated proteins revealed differences in arrangement of SPCA1 domains that may have functional consequences. Strikingly, the phosphorylation and the nucleotide-binding domains were interrupted by insertion of a partial actuator, transmembrane, and phosphorylation domains. The additional 294 amino acids appear to disrupt SPCA1 structure and function, causing HHD. Our study expands the spectrum of genetic defects in HHD and showed that disruption of SPCA1 structure and function by the microduplication caused HHD in the patient and her father.

3.
Sci Rep ; 7(1): 8212, 2017 08 15.
Article in English | MEDLINE | ID: mdl-28811604

ABSTRACT

Monoclonal antibodies offer great tools for research. We encountered a potentially useful mouse IgM monoclonal antibody whose antigen is expressed in normal skin but lost in human skin cancer. Because IgM is difficult to work with and the antigen was unknown, we decided to convert the IgM (µ) to IgG (γ) version. After cDNA for the antibody was obtained by RACE PCR, we made a series of molecules with different combinations of IgM and IgG domains. Whereas VH-Cµ1-Cµ2-Cγ3 and VH-Cµ1-Cµ2-Hinge-Cγ2-Cγ3 functionally bound to the antigen, VH-Cγ1-Hinge-Cγ2-Cγ3, VH-Cµ1-Hinge-Cγ2-Cγ3, and VH-Cµ1-Cµ2-Cγ2-Cγ3 did not. Gel filtration analyses revealed that the functional molecules tend to form multimers and the multimeric forms retained antigen binding activity. Furthermore, the mutation of amino acid residue p.309Q > C of mouse IgG and addition of IgM tailpiece to the C-terminus of the molecules induced multimer formation, dramatically enhanced antibody functionality and all non-functional molecules became strongly functional. The functional molecules could be bound by protein A/protein G and other IgG specific reagents and therefore should be useful for further characterization of the antigen. Our study revealed that multimerization of converted IgM is functionally important for antigen binding activity of engineered IgM/IgG chimeric antibodies.


Subject(s)
Antibodies, Monoclonal/immunology , Antigens/immunology , Immunoglobulin G/immunology , Immunoglobulin M/immunology , Skin/immunology , Animals , Antibodies, Monoclonal/genetics , Antigens/chemistry , Fluorescent Antibody Technique , Humans , Immunoglobulin G/genetics , Immunoglobulin Heavy Chains/genetics , Immunoglobulin M/genetics , Immunoglobulin Variable Region/genetics , Mice , Mutation , Protein Binding/immunology , Protein Multimerization , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/immunology
5.
PLoS One ; 11(8): e0160952, 2016.
Article in English | MEDLINE | ID: mdl-27505250

ABSTRACT

CD44, a cell surface proteoglycan, is involved in many biological events. CD44 transcripts undergo complex alternative splicing, resulting in many functionally distinct isoforms. To date, however, the nature of these isoforms in human epidermis has not been adequately determined. In this study, we isolated all CD44 transcripts from normal human epidermis, and studied how their expressions are regulated. By RT-PCR, we found that a number of different CD44 transcripts were expressed in human epidermis, and we obtained all these transcripts from DNA bands in agarose and acrylamide gels by cloning. Detailed sequence analysis revealed 18 CD44 transcripts, 3 of which were novel. Next, we examined effects of 10 different agents on the expression of CD44 transcripts in cultured human keratinocytes, and found that several agents, particularly epidermal growth factor, hydrogen peroxide, phorbol 12-myristate 13-acetate, retinoic acid, calcium and fetal calf serum differently regulated their expressions in various patterns. Furthermore, normal and malignant keratinocytes were found to produce different CD44 transcripts upon serum stimulation and subsequent starvation, suggesting that specific CD44 isoforms are involved in tumorigenesis via different CD44-mediated biological pathways.


Subject(s)
Epidermis/metabolism , Gene Expression Regulation/drug effects , Hyaluronan Receptors/genetics , Adult , Animals , Base Sequence , CHO Cells , Cricetinae , Cricetulus , Epidermal Cells , Exons/genetics , Genetic Variation , Humans , Keratinocytes/cytology , Keratinocytes/metabolism , Keratinocytes/pathology , Protein Isoforms/genetics , RNA, Messenger/genetics , RNA, Messenger/isolation & purification , Skin Neoplasms/pathology
10.
J Invest Dermatol ; 136(2): 399-408, 2016 Feb.
Article in English | MEDLINE | ID: mdl-26802236

ABSTRACT

All plakin family proteins are known to be autoantigens in paraneoplastic pemphigus (PNP). In this study, we first examined whether PNP sera also react with epiplakin, another plakin protein, by various immunological methods using 48 Japanese PNP sera. Immunofluorescence confirmed that cultured keratinocytes expressed epiplakin. Epiplakin was detected by 72.9% of PNP sera by immunoprecipitation-immunoblotting with KU-8 cell extract, but not by immunoblotting of either normal human epidermal extract or KU-8 cell extract. Epiplakin was essentially not detected by 95 disease and normal control sera. Statistical analyses of various clinical and immunological findings revealed a significant correlation of the presence of anti-epiplakin antibodies with both bronchiolitis obliterans and mortality. No epiplakin-negative PNP case developed bronchiolitis obliterans. However, although 29.4% of European patients with PNP had bronchiolitis obliterans, significant correlation with anti-epiplakin autoantibodies was not observed. In further studies for lung, immunofluorescence showed the presence of epiplakin in normal human lung, particularly respiratory bronchiole, immunoprecipitation-immunoblotting showed that PNP sera reacted with epiplakin in cultured lung cells, and mice injected with polyclonal antibody specific to epiplakin histopathologically showed abnormal changes in small airway epithelia. These results indicated that epiplakin is one of the major PNP autoantigens and is related to PNP-related bronchiolitis obliterans.


Subject(s)
Autoantigens/immunology , Autoantigens/metabolism , Bronchiolitis Obliterans/immunology , Paraneoplastic Syndromes/immunology , Pemphigus/immunology , Aged , Animals , Asian People/statistics & numerical data , Autoantibodies/blood , Biomarkers/blood , Bronchiolitis Obliterans/ethnology , Bronchiolitis Obliterans/metabolism , Cells, Cultured , Female , Fluorescent Antibody Technique , Humans , Immunoprecipitation , Keratinocytes/immunology , Keratinocytes/metabolism , Male , Mice , Middle Aged , Paraneoplastic Syndromes/ethnology , Paraneoplastic Syndromes/metabolism , Pemphigus/ethnology , Pemphigus/metabolism , Rats , Reference Values , Sampling Studies , Statistics, Nonparametric
11.
Chin Med J (Engl) ; 128(16): 2228-33, 2015 Aug 20.
Article in English | MEDLINE | ID: mdl-26265618

ABSTRACT

BACKGROUND: Shikonin is a major active chemical component extracted from Lithospermi Radix, an effective traditional herb in various types of wound healing. Shikonin can accelerate granulomatous tissue formation by the rat cotton pellet method and induce neovascularization in granulomatous tissue. The purpose of the study was to investigate its mechanism of action in human skin cells. METHODS: MTS assay was used to measure cell growth. The collagen type I (COL1 ) mRNA expression and procollagen type I C-peptide (PIP) production were detected by real-time quantitative reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Immunofluorescence and western blot analyses were carried out to investigate nuclear factor-κB (NF-κB) signaling pathway. Cell-based proteasome activity assay was used to determine proteasome activity. RESULTS: In this study, we found that 10 µmol/L shikonin stimulated the growth of normal human keratinocytes and 1 µmol/L shikonin promoted growth of human dermal fibroblasts. However, shikonin did not directly induce COL1 mRNA expression and PIP production in dermal fibroblasts in vitro. In addition, 1 µmol/L shikonin inhibited translocation of NF-κB p65 from cytoplasm to nucleus induced by tumor necrosis factor-α stimulation in dermal fibroblasts. Furthermore, shikonin inhibited chymotrypsin-like activity of proteasome and was associated with accumulation of phosphorylated inhibitor κB-α in dermal fibroblasts. CONCLUSIONS: These results suggested that shikonin may promote wound healing via its cell growth promoting activity and suppress skin inflammation via inhibitory activity on proteasome. Thus, shikonin may be a potential therapeutic reagent both in wound healing and inflammatory skin diseases.


Subject(s)
Cell Proliferation/drug effects , NF-kappa B/metabolism , Naphthoquinones/pharmacology , Proteasome Endopeptidase Complex/drug effects , Skin/cytology , Cells, Cultured , Enzyme-Linked Immunosorbent Assay , Fibroblasts/drug effects , Humans , Keratinocytes/drug effects , Polymerase Chain Reaction
14.
Exp Dermatol ; 24(1): 62-5, 2015 Jan.
Article in English | MEDLINE | ID: mdl-25346431

ABSTRACT

Peroxisome proliferator-activated receptors (PPARs) are potentially useful for the treatment of skin diseases, because they stimulate keratinocyte differentiation, exert anti-inflammatory effects and improve barrier function. We examined five PPAR-γ agonists, including four thiazolidinediones (ciglitazone, troglitazone, rosiglitazone and pioglitazone) and an angiotensin-II receptor blocker (telmisartan), for their ability to upregulate filaggrin and loricrin expression at both mRNA and protein levels in cultured normal human keratinocytes (NHKs). Troglitazone, rosiglitazone, pioglitazone and telmisartan significantly increased filaggrin expression at both mRNA and protein levels in calcium-induced differentiated NHKs. Rosiglitazone and pioglitazone, but not troglitazone nor telmisartan, also significantly increased loricrin expression at both mRNA and protein levels in differentiated NHKs. These effects were not found in undifferentiated NHKs nor differentiated NHKs treated with ciglitazone. This study revealed differential effects of various PPAR-γ agonists on epidermal differentiation, and the most potent of those are rosiglitazone and pioglitazone.


Subject(s)
Gene Expression Regulation/drug effects , Intermediate Filament Proteins/chemistry , Keratinocytes/drug effects , Membrane Proteins/chemistry , PPAR gamma/agonists , Thiazolidinediones/chemistry , Anti-Inflammatory Agents/chemistry , Benzimidazoles/chemistry , Benzoates/chemistry , Cell Differentiation/drug effects , Cells, Cultured , Chromans/chemistry , Filaggrin Proteins , Humans , Keratinocytes/cytology , Microscopy, Fluorescence , Pioglitazone , RNA, Messenger/metabolism , Rosiglitazone , Skin/drug effects , Telmisartan , Troglitazone
16.
J Dermatol Sci ; 75(1): 36-42, 2014 Jul.
Article in English | MEDLINE | ID: mdl-24794518

ABSTRACT

BACKGROUND: Peeling skin syndrome (PSS) is a rare autosomal recessive form of ichthyosis showing skin exfoliation. PSS is divided into acral and generalized PSS, and the latter is further classified into non-inflammatory type (PSS type A) and inflammatory type (PSS type B). PSS type B is now called peeling skin disease (PSD). Different loss-of-function mutations in the corneodesmosin (CDSN) gene have been reported to cause PSD. OBJECTIVE: The aim of this study was to determine genetic basis of disease in a 14-year-old Japanese patient with PSD. METHODS AND RESULTS: Immunohistochemical study showed lack of corneodesmosin (CDSN) in the skin, and standard PCR for genomic DNA failed to amplify CDSN product, suggesting CDSN defect. Multiplex ligation-dependent probe amplification and genomic quantitative real-time PCR analyses detected large homozygous deletion of 59,184bp extending from 40.6kb upstream to 13.2kb downstream of CDSN, which included 6 genes (TCF19, CCHCR1, PSORS1C2, PSORS1C1, CDSN and C6orf15). The continuous gene lost did not result in additional clinical features. Inverted repeats with 85% similarity flanking the deletion breakpoint were considered to mediate the deletion by non-homologous end joining or fork stalling and template switching/microhomology-mediated break-induced replication. Parents were clinically unaffected and were heterozygote carriers of the same deletion, which was absent in 284 ethnically matched control alleles. We also developed simple PCR method, which is useful for detection of this deletion. CONCLUSION: Although 5 other genes were also deleted, homozygous deletion of CDSN was considered to be responsible for this PSD.


Subject(s)
Chromosomes, Human, Pair 6 , Dermatitis, Exfoliative/genetics , Gene Deletion , Glycoproteins/genetics , Homozygote , Skin Diseases, Genetic/genetics , Adolescent , Biopsy , Dermatitis, Exfoliative/diagnosis , Dermatitis, Exfoliative/metabolism , Genetic Predisposition to Disease , Genetic Testing/methods , Glycoproteins/analysis , Humans , Immunohistochemistry , Intercellular Signaling Peptides and Proteins , Male , Phenotype , Polymerase Chain Reaction , Skin/chemistry , Skin/pathology , Skin Diseases, Genetic/diagnosis , Skin Diseases, Genetic/metabolism
17.
Exp Dermatol ; 23(7): 514-6, 2014 Jul.
Article in English | MEDLINE | ID: mdl-24698124

ABSTRACT

Hailey-Hailey disease (HHD) is a dominantly inherited skin disease caused by mutations in ATP2C1 gene, which encodes secretory pathway Ca(2+) /Mn(2+) -ATPase protein 1. The precise mechanism remains unclear. In this study, to understand molecular basis of HHD, we examined expression of mRNA and protein in cultured keratinocytes derived from three HHD patients with different mutations. We showed that reduced expression of mRNA and protein in patient with p.Gln504X, but not in patients with p.Pro307His and c.1308+1G>A. RT-PCR analysis for patient with c.1308+1G>A revealed in-frame exon skipping. Reduction of mRNA and protein in p.Gln504X was considered to be caused by nonsense-mediated mRNA decay. p.Pro307His located adjacent to Ca(2+) -binding residue may induced conformational change, which leads to defective Ca(2+) transport. In-frame shorter transcript caused by c.1308+1G>A may have slightly reduced activity, which accounted for mild phenotype of the patient. These results clarified the pathogenic effects of different causative mutations in development of skin lesions.


Subject(s)
Keratinocytes/metabolism , Mutation , Pemphigus, Benign Familial/genetics , RNA, Messenger/metabolism , Aged , Aged, 80 and over , Calcium/metabolism , Calcium-Transporting ATPases/metabolism , Cells, Cultured , Codon, Nonsense , Cytoplasm/metabolism , Exons , Gene Deletion , Humans , Keratinocytes/cytology , Male , Middle Aged , Pemphigus, Benign Familial/metabolism , Phosphorylation , Protein Structure, Tertiary
19.
J Invest Dermatol ; 133(7): 1785-93, 2013 Jul.
Article in English | MEDLINE | ID: mdl-23407400

ABSTRACT

Paraneoplastic pemphigus (PNP) shows autoantibodies mainly to plakin and desmosomal cadherin family proteins. We have recently identified alpha-2-macroglobulin-like-1 (A2ML1), a broad range protease inhibitor, as a unique PNP antigen. In this study, we tested a large number of PNP sera by various methods. Forty (69.0%) of 58 PNP sera recognized A2ML1 recombinant protein expressed in COS7 cells by immunofluorescence (IF) and/or immunoprecipitation (IP)/immunoblotting (IB). IP/IB showed higher sensitivity than IF. In addition, 22 (37.9%) PNP sera reacted with A2ML1 by IB of cultured normal human keratinocytes (NHKs) under non-reducing conditions. Statistical analyses using various clinical and immunological data showed that the presence of anti-A2ML1 autoantibodies was associated with early disease onset and absence of ocular lesions. Next, to investigate the pathogenic role of anti-A2ML1 antibody, we performed additional functional studies. Addition of anti-A2ML1 polyclonal antibody to culture media decreased NHK cell adhesion examined by dissociation assay, and increased plasmin activity detected by casein zymography, suggesting that anti-A2ML1 antibody may decrease NHK cell adhesion through plasmin activation by inhibition of A2ML1. This study demonstrates that autoantibodies to A2ML1 are frequently and specifically detected and may have a pathogenic role in PNP.


Subject(s)
Autoantibodies/physiology , Paraneoplastic Syndromes/etiology , Paraneoplastic Syndromes/physiopathology , Pemphigus/etiology , Pemphigus/physiopathology , alpha-Macroglobulins/immunology , Adolescent , Adult , Aged , Animals , Autoantibodies/blood , Autoantibodies/pharmacology , COS Cells , Cell Adhesion/drug effects , Cells, Cultured , Child , Chlorocebus aethiops , Disease Models, Animal , Fibrinolysin/metabolism , Fluorescent Antibody Technique , Humans , Immunoprecipitation , Keratinocytes/drug effects , Keratinocytes/metabolism , Keratinocytes/pathology , Middle Aged , Rats , Transfection , Young Adult , alpha-Macroglobulins/genetics
20.
Exp Dermatol ; 22(2): 83-7, 2013 Feb.
Article in English | MEDLINE | ID: mdl-23039137

ABSTRACT

Inherited keratinizing disorders are caused by mutations in the genes encoding cornified cell envelope proteins, enzymes and their inhibitors, adhesion molecules, cytoskeletal proteins and others in the epidermis. These molecules are known to regulate differentiation, proliferation and cell adhesions. Intriguingly, some keratinizing disorders show blistering skin lesions, while some inherited blistering disorders show abnormal keratinization. Therefore, hereditary keratinizing and blistering diseases are closely related and show overlapping genetic backgrounds. In this review, we overviewed keratinizing and blistering disorders in terms of overlapping of the two disease groups. Gene mutations in desmosomal components cause striate keratoderma, Naxos disease, epidermolytic palmoplantar keratoderma and plakophilin deficiency, which first show skin fragility and blisters and later hyperkeratosis. Gene mutations in hemidesmosomal components cause various forms of epidermolysis bullosa, some of which show hyperkeratosis on the nails, palms and soles, in addition to blister formation. Diseases with gene mutations in calcium pump proteins are Darier disease and Hailey-Hailey disease, which show clinicopathological overlaps and develop both keratinizing and blistering skin lesions. Finally, gene mutations in epidermal keratins cause epidermolysis bullosa simplex, epidermolytic ichthyosis, superficial epidermolytic ichthyosis, epidermolytic palmoplantar keratoderma and pachyonychia congenita/focal palmoplantar keratoderma, which show thickening of the palms and soles with underlying blister formation. In general, responsible proteins for diseases developing both keratinizing and blistering conditions are adhesion molecules, calcium pump proteins and keratins, but not connexins, cornified cell envelop proteins, enzymes or inhibitors. It is still unknown how particular keratinizing diseases develop blisters and vice versa.


Subject(s)
Blister/genetics , Epidermis/pathology , Hyperkeratosis, Epidermolytic/genetics , Keratins/genetics , Keratoderma, Palmoplantar/genetics , Skin Diseases/genetics , Arrhythmogenic Right Ventricular Dysplasia/genetics , Calcium/metabolism , Cell Differentiation , Epidermis/metabolism , Epidermolysis Bullosa/genetics , Hair Diseases/genetics , Humans , Keratins/physiology , Mutation , Pemphigus, Benign Familial/genetics
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