ABSTRACT
Data on the hemolysin synthesis determined by the plasmid pHly241, belonging to the incompatibility group I2, and its derivatives are presented in this paper. The restriction analysis of the pHly241 plasmid has resulted in the detailed mapping of the hly determinant region in the plasmid. The substantial homology has been demonstrated between the regions of the plasmids pHly241 and pHly195 coding for hemolysin synthesis and excretion from the bacterial cell.
Subject(s)
Base Sequence , Escherichia coli/genetics , Genes, Bacterial , Hemolysin Factors , Plasmids , Sequence Homology, Nucleic Acid , Chromosome Mapping , DNA Restriction Enzymes , MutationSubject(s)
Escherichia coli/genetics , Hemolysin Factors , Plasmids , Cloning, Molecular , Genes , Molecular WeightSubject(s)
Cerebrovascular Circulation , Epilepsy/physiopathology , Syncope/physiopathology , Adolescent , Adult , Brain/blood supply , Female , Hemodynamics , Humans , Male , Middle Aged , Plethysmography, ImpedanceABSTRACT
Bac. subtilis 168 (BD-25) cells were infected with DNA of plasmide R1drd19 isolated from E. coli strain; transformants resistant to streptomycin (500 microgram/ml) and kanamycin (40 microgram/ml) appeared with the frequency of 2.10(-6). These transformants retained resistance to the mentioned antibiotics stably. A satellite DNA peak was revealed in centrifugation in the density gradient of cesium chloride with ethidium bromide. It was possible to infect cells of Bac. subtilis 168 (BD-25) with plasmide DNA isolated from the transformants. Plasmide transduction with the aid of phages AR9 and PBSI multiplied on the transformant strains was also effected. Physico-chemical analysis of the transformed plasmide DNA was conducted; its molecular weight was determined.
