ABSTRACT
The study evaluated the effect of the supernatant of placental explants from preeclamptic (PE) and normotensive (NT) pregnant women after tissue treatment with or without vitamin D (VD) on oxidative stress and nitric oxide (NO) bioavailability in human umbilical vein endothelial cells (HUVEC). Placental explants were prepared from eight NT and eight PE women, and supernatants were obtained after incubation with or without hydrogen peroxide (H2O2) and/or VD. HUVEC were cultured for 24 h with supernatants, and the following parameters were analyzed in HUVEC cultures: NO, nitrate (NO3-), and nitrite (NO2-) levels, lipid peroxidation, and intracellular reactive oxygen species (ROS). Results showed that the production of NO3-, NO2-, malondialdehyde (MDA), and ROS were significantly higher in HUVEC treated with explant supernatant from PE compared to NT pregnant women, while the supernatant of PE explants treated with VD led to a decrease in these parameters. A significantly high production of NO was detected in HUVEC cultured with control supernatant of NT group, and in cultures treated with supernatant of PE explants treated with VD. Taken together, these results demonstrated that cultures of placental explants from PE women with VD treatment generated a supernatant that decreased oxidative stress and increased the bioavailability of NO in endothelial cells.
Subject(s)
Nitric Oxide , Pre-Eclampsia , Biological Availability , Cells, Cultured , Female , Human Umbilical Vein Endothelial Cells , Humans , Hydrogen Peroxide , Nitric Oxide/metabolism , Oxidative Stress , Placenta/metabolism , Pre-Eclampsia/metabolism , Pregnancy , Vitamin D/metabolismABSTRACT
The study evaluated the effect of the supernatant of placental explants from preeclamptic (PE) and normotensive (NT) pregnant women after tissue treatment with or without vitamin D (VD) on oxidative stress and nitric oxide (NO) bioavailability in human umbilical vein endothelial cells (HUVEC). Placental explants were prepared from eight NT and eight PE women, and supernatants were obtained after incubation with or without hydrogen peroxide (H2O2) and/or VD. HUVEC were cultured for 24 h with supernatants, and the following parameters were analyzed in HUVEC cultures: NO, nitrate (NO3-), and nitrite (NO2-) levels, lipid peroxidation, and intracellular reactive oxygen species (ROS). Results showed that the production of NO3-, NO2-, malondialdehyde (MDA), and ROS were significantly higher in HUVEC treated with explant supernatant from PE compared to NT pregnant women, while the supernatant of PE explants treated with VD led to a decrease in these parameters. A significantly high production of NO was detected in HUVEC cultured with control supernatant of NT group, and in cultures treated with supernatant of PE explants treated with VD. Taken together, these results demonstrated that cultures of placental explants from PE women with VD treatment generated a supernatant that decreased oxidative stress and increased the bioavailability of NO in endothelial cells.
Subject(s)
Humans , Female , Pregnancy , Pre-Eclampsia/metabolism , Nitric Oxide/metabolism , Placenta/metabolism , Vitamin D/metabolism , Biological Availability , Cells, Cultured , Oxidative Stress , Human Umbilical Vein Endothelial Cells , Hydrogen PeroxideABSTRACT
AIM: Sex differences in exercise responses have implications for understanding sex-specific adaptations to exercise for performance and health. The purpose of this study was to verify the acute effects of a full body resistance exercise protocol on growth hormone (GH), testosterone (TT), cortisol, interleukin (IL)-6 and IL-10 in men and women. METHODS: Fourteen healthy volunteers (7 men and 7 women taking oral contraceptives) active and recreationally trained in resistance exercise were subjected to a resistance exercise session (3x8-10 RM) composed of 10 exercises with rests periods of 90-120 seconds between sets. GH, TT, cortisol, IL-6 and IL-10 were assessed at pre-, immediate post- (IP) and 30 min postprotocol. RESULTS: Both men and women had a similar increase in GH (P<0.05) at IP in response to exercise. Significant effects of interaction between sex and time were observed for TT, cortisol and IL-6. In the men, an increase from pre was noted at IP and 30 min for TT, cortisol and IL-6. In the women there was no change in TT, cortisol and IL-6 concentration. There was no change in IL-10. CONCLUSION: Our findings indicate a difference between men and women taking oral contraceptives in TT, cortisol and IL-6 responsiveness to the same full body resistance exercise protocol.
