ABSTRACT
UNLABELLED: In the present work, 3 different yeast strains (1, 2, and 3) were used to elaborate white wines using Albariño must. The concentration of polymeric mannose was determined using a method based on the mannoprotein precipitation, hydrolysis and analysis of sylylated mannose derivatives by gas chromatography. Wines elaborated with the strain 1 (W1) presented a higher mannoprotein concentration than the other wines. The analysis of the volatile composition of wines showed significant differences (P < 0.05) among them, being W1 which presented the highest concentration of aroma compounds, mainly terpens and norisoprenoids. The sensorial analysis of wines also showed that W1 had the best quality. The results obtained from this work demonstrate that mannoproteins could be involved in the behavior observed. Some evidences were obtained using a model wine, where 2 major terpens in W1 were preferentially retained by the colloids rich in mannoproteins released by strain 1. PRACTICAL APPLICATION: White wines elaborated with yeast strains overproducing mannoproteins could have better quality than others. Mannoproteins could contribute to aroma enhancement of Albariño white wines.
Subject(s)
Food Microbiology , Fungal Proteins/biosynthesis , Membrane Glycoproteins/biosynthesis , Saccharomyces cerevisiae/metabolism , Wine/microbiology , Acyclic Monoterpenes , Chromatography, Gas , Fungal Proteins/analysis , Humans , Mannose/analysis , Membrane Glycoproteins/analysis , Monoterpenes/isolation & purification , Monoterpenes/metabolism , Odorants/analysis , Smell , Terpenes/isolation & purification , Terpenes/metabolism , Vitis/chemistry , Vitis/microbiology , Volatile Organic Compounds/analysis , Wine/analysisABSTRACT
A wine model was evaluated to determine the influence of aging on the ability of whole yeast cells (WY) and yeast cell walls (YCW) to remove ochratoxin A (OTA). Aging and autolysis were monitored for 214 h in the model wine. The original concentration of OTA in the model wine was 10 microg/liter, and WY and YCW were added at a final concentration of 1 g/liter. YCW mannoproteins were involved in the removal of OTA from the model wine through adsorption mechanisms. Aging affected the capacity of WY to remove OTA, but YCW removal capacity remained constant during aging. A previous heat treatment (85 degrees C for 10 min) of WY and YCW increased their removal capacity and increased the efficiency of the decontamination process.
Subject(s)
Decontamination/methods , Food Contamination/analysis , Ochratoxins/analysis , Saccharomyces cerevisiae/physiology , Wine/analysis , Adsorption , Autolysis , Hot Temperature , Humans , Industrial Microbiology , Time Factors , Vitis/chemistry , Vitis/microbiology , Wine/microbiologyABSTRACT
Two different yeast cell wall extracts were obtained using enzymatic digestion and thermal treatment. The effects of the extracts obtained on the foaming properties of a model wine and two sparkling wines were studied. The model wine and sparkling wines, supplemented with the thermal extract, presented better foaming properties than did the samples supplemented with the enzymatic extract. The fractioning (Con A chromatography) and characterization (SDS-PAGE, SEC, GC, and RP-HPLC) of both extracts showed that the fraction responsible for the foaming properties is constituted by mannoproteins with a relative molecular weight between 10 and 30 kDa, presenting an equilibrated composition of the hydrophobic and hydrophilic protein domains. This thermal extract did not modify the protein stability in both the model wine and the sparkling wines. These results demonstrate that the enrichment of a sparkling wine with mannoproteins extracted by mild heat procedures will contribute to improving its foaming properties.