ABSTRACT
HER-2 codon 655 polymorphism together with human papillomavirus (HPV) types were examined in a total of 279 cervical smear samples. Forty-nine patients with high-grade squamous intraepithelial lesion had higher frequency of high-risk HPV than 167 patients with low-grade squamous intraepithelial lesion and 63 controls. There was no statistical difference in the frequencies of HER-2 Ile/Ile, Ile/Val, and Val/Val genotypes between squamous intraepithelial lesions (SILs) and controls. When the Ile/Ile genotype was compared to the Ile/Val + Val/Val genotypes, there was also no statistical difference in the genotype prevalence between SILs and controls either in 91 or 188 patients with or without high-risk HPV, respectively. These results suggest that the HER-2 polymorphism at codon 655 in cervical cell samples is unlikely to be associated with HPV status and the onset of cervical cancer in a Japanese population.
Subject(s)
Carcinoma, Squamous Cell/genetics , Papillomavirus Infections/diagnosis , Polymorphism, Genetic , Uterine Cervical Neoplasms/genetics , Uterine Cervical Neoplasms/pathology , Base Sequence , Carcinoma, Squamous Cell/epidemiology , Carcinoma, Squamous Cell/pathology , Case-Control Studies , Chi-Square Distribution , Codon , Female , Gene Expression Regulation, Neoplastic , Genes, erbB-2 , Genotype , Humans , Incidence , Japan/epidemiology , Mass Screening , Molecular Sequence Data , Neoplasm Staging , Papillomaviridae/isolation & purification , Papillomavirus Infections/epidemiology , Risk Assessment , Sampling Studies , Sensitivity and Specificity , Statistics, Nonparametric , Uterine Cervical Neoplasms/epidemiology , Vaginal SmearsABSTRACT
OBJECTIVE: To determine the usefulness of the combination of confocal laser scanning microscopy (CLSM), image cytometry and three-dimensional (3D) imaging for analyzing architectural changes indicative of endometrial hyperplasia and grade 1 adenocarcinoma. STUDY DESIGN: Papanicolaou-stained endometrial samples (n = 180) were analyzed for specific cellular characteristics and analyzed by CLSM. Confocal images were obtained and then analyzed cytometrically and used for 3D reconstruction. RESULTS: Values obtained after image cytometry and 3D imaging increased significantly (P < .01) with the degree of cellular atypia. CONCLUSION: The combination of CLSM, image cytometry and 3D imaging is a valuable method for differential diagnosis of endometrial hyperplasia and grade 1 adenocarcinoma.
Subject(s)
Adenocarcinoma/pathology , Endometrial Neoplasms/pathology , Image Processing, Computer-Assisted/methods , Microscopy, Confocal/methods , Adult , Cell Nucleus/pathology , Female , Humans , Hyperplasia/pathology , Middle AgedABSTRACT
OBJECTIVE: To establish standardized Papanicolaou stain for cytology using RGB color specification. This new method was formerly used in DTP software application for computer color specification. STUDY DESIGN: RGB color specification was taken from a color film, optical constituents of which were made into computer software. Cell samples used in this study were from 100 sputum specimens stained with Papanicolaou stain. We analyzed the color tone of the cytoplasm of squamous cells in the smear. RESULTS: The R and B value of eosinophilic cells were demonstrated statistically by different values between normal, borderline atypical and malignant squamous cells. G and B values of light green-philic cells demonstrated a statistical difference between normal, borderline atypical and malignant squamous cells. No significant differences were found in RGB value between normal, borderline atypical and malignant squamous orangeophilic cells. CONCLUSION: Using our own method of analyzing Papanicolaou-stained sputum, a new quantitative and qualitative analysis of stain color for standardized Papanicolaou stain was introduced.
Subject(s)
Color , Image Processing, Computer-Assisted/methods , Software , Carcinoma, Squamous Cell/chemistry , Carcinoma, Squamous Cell/pathology , Cytoplasm/chemistry , Humans , Image Processing, Computer-Assisted/standards , Sputum/chemistry , Staining and Labeling/methods , Staining and Labeling/standards , Vaginal Smears/methods , Vaginal Smears/standardsABSTRACT
Comparison and quality control of our experience of thyroid cytology (784 samples) and breast cytology (1,202 samples) were reported. False positive rate and other parameters were superior in breast cytology than thyroid. It may be due to the fact that cytological diagnostic criteria for follicular thyroid carcinoma from follicular adenoma is not established yet. More suspicious diagnosis in thyroid than breast also give low incidence of malignancy in thyroid suspicious diagnosis. As recommended by a guideline for both thyroid and breast cytology from the Papanicolaou society et al, conservative handling and protective diagnosis may help to reduce false positive and false negative rates.
