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J Clin Lab Anal ; 30(6): 1236-1246, 2016 Nov.
Article in English | MEDLINE | ID: mdl-27346210

ABSTRACT

BACKGROUND: Risperidone (RIS) is a widely used atypical antipsychotic drug. We developed and validated a sensitive and accurate LC-MS/MS method, which requires a small-volume of plasma and small-volume injection for measurement of RIS levels in ASD pediatric patients. We also investigated the relationship between RIS levels and RIS dosages, including prolactin levels. METHOD: Blood samples were processed by protein precipitation extraction. Only 1 µl of sample was injected. Plasma samples were separated on a C18 column (4.6 cm × 50 mm; 1.8 µm particle size). Detection was by MS-MS with an analytical run time of 6 min. RESULTS: The inter-day accuracy of RIS was 101.33-107.68% and 95.24-103.67% for 9-OH-RIS. The inter-day precision of RIS was ≤7.27% CV and ≤7.41% CV for 9-OH-RIS. The extraction recovery of RIS and 9-OH-RIS were 95.01 ± 7.31-112.62 ± 7.50% and 90.27 ± 11.15-114.00 ± 10.35%, respectively. This method was applied in the therapeutic drug monitoring of ASD pediatric patients. Higher RIS dosage has a tendency to produce higher RIS plasma levels. The high RIS plasma levels have a tendency to produce hyperprolactinemia. CONCLUSION: The determination of RIS in individual patients might be clinically useful for monitoring and prediction of treatment response.


Subject(s)
Antipsychotic Agents/blood , Autism Spectrum Disorder/blood , Chromatography, Liquid , Drug Monitoring/methods , Paliperidone Palmitate/blood , Risperidone/blood , Tandem Mass Spectrometry , Antipsychotic Agents/therapeutic use , Autism Spectrum Disorder/drug therapy , Female , Humans , Male , Paliperidone Palmitate/therapeutic use , Risperidone/therapeutic use , Statistics as Topic , Statistics, Nonparametric
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