Gastrointestinal toxicity of melos conquer mixture in rats.

GIT effects of Melos Conquer Mixture were investigated - using albino wister rats. Conquer Mixture was administered orally at different concentrations whereas the control group received water. The experiment lasted from 3 to 21 days. Pathological examination was carried out on the dead animals. The animals showed (1) loss of weight and appetite (2) weakness (3) faeces was soft (4) out of 10 animals which received 1.20 ml/kg died on day 5(5) the Glt of the dead rats was virtually empty except in the colon, (6) pieces from various parts of GIT revealed evidence of acute and sub acute inflammatory cellular reactions. The results indicate that Conquer Mixture may be toxic to the gastrointestinal tract and suggest that a re-evaluation of the therapeutic usefulness of the drug in the management of malaria is warranted.

Calcitonin fragmentation by procine tissue in vitro characterized by gel filtration chromatography.

The cytosolic fraction of porcine thyroid, liver, kidney and brain tissue contains proteolytic activity capable of calcitonin degradation into peptide fragments. The degree and pattern of calcitonin fragmentation produced by the tissue examined in this study were determined by subjecting aliquots of incubation media to gel filtration chromatography immediately after incubation. Thyroid cytosol produced calcitonin fragmentation in a time-dependent manner. Fragmentation produced by liver, kidney and brain appeared to be quantitatively and qualitatively different from that produced by the thyroid. Hormone degradation produced by thyroid cytosol was substantially inhibited by p-hydroxymercuribenzoate, thimerosal and iodoacetamide but not by pepstatin. The thyroid cytosolic peptidase may be involved in calcitonin turnover and presecretory regulation within the "C' cell. Further, fragmentation within the thyroid may be responsible for the multiple immunoreactive forms of calcitonin which have been detected in the circulation.