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1.
PART I: FIGHTING CANCER WITH DEADLY BACTERIA.
Nybo, Kristie.
Biotechniques ; 64(1): 6-8, 2018 01 01.
Article in English | MEDLINE | ID: mdl-29384070

ABSTRACT

A 200-year-old observation might provide a new way to eliminate tumors by infecting cancer patients with bacteria. Kristie Nybo explores a new approach that could transform cancer treatment.


Subject(s)
Immunity , Neoplasms/complications , Neoplasms/therapy , Salmonella Infections/complications , Salmonella/immunology , Animals , Biological Therapy/methods , Clinical Trials as Topic , Humans , Neoplasms/immunology , Salmonella/genetics , Salmonella/ultrastructure , Salmonella Infections/immunology , Salmonella typhimurium/genetics , Salmonella typhimurium/immunology , Salmonella typhimurium/ultrastructure
2.
Following a hunch.
Xie, Xiaoliang Sunney; Nybo, Kristie.
Biotechniques ; 56(4): 163, 2014.
Article in English | MEDLINE | ID: mdl-24839668

Subject(s)
Molecular Biology , Cholesterol Oxidase , Humans , Massachusetts , Nucleic Acid Amplification Techniques , Spectrum Analysis, Raman
3.
Scientists. Excitement for a new era.
Lander, Eric; Nybo, Kristie.
Biotechniques ; 56(3): 107, 2014.
Article in English | MEDLINE | ID: mdl-24779058

Subject(s)
Human Genome Project/history , History, 20th Century , History, 21st Century , United States
4.
Troubleshooting forum. SDS-page. What is the best way to separate proteins of similar molecular weight? (Thread 33111).
Nybo, Kristie.
Biotechniques ; 56(3): 115, 2014.
Article in English | MEDLINE | ID: mdl-24779059

Subject(s)
Electrophoresis, Polyacrylamide Gel/methods , Proteins/isolation & purification , Molecular Weight , Proteins/chemistry
5.
Laser capture microdissection.
Nybo, Kristie.
Biotechniques ; 56(1): 17, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24592490

Subject(s)
Cell Separation , Laser Capture Microdissection/methods , Molecular Biology/methods , Humans , RNA/genetics
6.
Troubleshooting forum. Molecular biology techniques Q&A.
Nybo, Kristie.
Biotechniques ; 56(2): 59, 2014.
Article in English | MEDLINE | ID: mdl-24660226

Subject(s)
Cell Separation/methods , Centrifugation/methods , Ficoll/chemistry , Laboratory Chemicals/chemistry
7.
Scientists. Curiosity and observation.
Zoghbi, Huda; Nybo, Kristie.
Biotechniques ; 55(2): 53, 2013 Aug.
Article in English | MEDLINE | ID: mdl-24079024

Subject(s)
Spinocerebellar Ataxias/history , Biomedical Research/history , Chromosome Mapping/history , Exploratory Behavior , History, 20th Century , History, 21st Century , Humans , Neurology/history , Pediatrics/history , Rett Syndrome/genetics , Rett Syndrome/history , Spinocerebellar Ataxias/genetics
8.
Viral expression.
Nybo, Kristie.
Biotechniques ; 55(1): 19-20, 2013 Jul.
Article in English | MEDLINE | ID: mdl-24000390

Subject(s)
Viruses/genetics , Viruses/metabolism , Animals , Genetic Vectors/genetics , Genetic Vectors/metabolism , Mice , Molecular Biology/methods , Molecular Biology/standards , NIH 3T3 Cells , Transfection
9.
Live cell imaging.
Nybo, Kristie.
Biotechniques ; 54(6): 307, 309, 2013 Jun.
Article in English | MEDLINE | ID: mdl-23905168

Subject(s)
Cell Culture Techniques/methods , Single-Cell Analysis/methods , Cell Culture Techniques/instrumentation , Culture Media , HEK293 Cells , Humans , Microinjections , Microscopy, Fluorescence , Single-Cell Analysis/instrumentation
10.
Primer design.
Nybo, Kristie.
Biotechniques ; 54(5): 249-50, 2013 May.
Article in English | MEDLINE | ID: mdl-23805429

Subject(s)
DNA Primers , Real-Time Polymerase Chain Reaction/methods , Computational Biology
11.
5' RACE.
Nybo, Kristie.
Biotechniques ; 54(4): 189-90, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23710526

ABSTRACT

This month's question from the Molecular Biology Forums (online at molecularbiology.forums.biotechniques.com) comes from the "RNA Methods" section. Entries have been edited for concision and clarity. Mentions of specific products and manufacturers have been retained from the original posts, but do not represent endorsements by, or the opinions of, BioTechniques.


Subject(s)
DNA, Complementary/genetics , Polymerase Chain Reaction/methods , RNA/genetics , 5' Untranslated Regions , DNA Primers/genetics , Genes, Plant , Genomics/methods , Plants/genetics , RNA, Plant/genetics
12.
qPCR: Single copy targets.
Nybo, Kristie.
Biotechniques ; 54(1): 22-3, 2013 Jan.
Article in English | MEDLINE | ID: mdl-23547283

Subject(s)
DNA Primers/chemistry , DNA Primers/genetics , DNA/analysis , DNA/genetics , Real-Time Polymerase Chain Reaction/methods , DNA/isolation & purification , Gene Dosage , Humans
13.
Molecular biology techniques Q&A.
Nybo, Kristie.
Biotechniques ; 53(4): 218-9, 2012 Oct.
Article in English | MEDLINE | ID: mdl-23227478

Subject(s)
Molecular Biology/methods , Proteins/isolation & purification
14.
qRT-PCR with saliva samples.
Nybo, Kristie.
Biotechniques ; 53(3): 137-8, 2012 Sep.
Article in English | MEDLINE | ID: mdl-22963475

Subject(s)
Real-Time Polymerase Chain Reaction/methods , Saliva/chemistry , Animals , DNA, Complementary/biosynthesis , DNA, Complementary/genetics , Gene Expression Profiling , Humans , RNA, Messenger/analysis , RNA, Messenger/genetics , RNA, Messenger/metabolism , RNA, Ribosomal, 18S/biosynthesis , RNA, Ribosomal, 18S/genetics , Real-Time Polymerase Chain Reaction/standards , Swine
15.
Retroviral transfection.
Nybo, Kristie.
Biotechniques ; 53(2): 79-80, 2012 Aug.
Article in English | MEDLINE | ID: mdl-22873513

Subject(s)
Genetic Vectors/genetics , Green Fluorescent Proteins/genetics , Retroviridae/genetics , Transfection/methods , Animals , Anti-Bacterial Agents/pharmacology , CHO Cells , COS Cells , Cell Line , Chlorocebus aethiops , Cricetinae , Green Fluorescent Proteins/analysis , Luminescent Agents/analysis , Luminescent Agents/metabolism , Mice , NIH 3T3 Cells , Neomycin/pharmacology , Viral Load
16.
Molecular biology techniques Q&A. Western blot: protein migration.
Nybo, Kristie.
Biotechniques ; 53(1): 23-4, 2012 Jul.
Article in English | MEDLINE | ID: mdl-22780315

Subject(s)
Antibodies/immunology , Blotting, Western/methods , Ion Channels/chemistry , Ion Channels/immunology , Animals , Antigen-Antibody Reactions , Electrophoresis, Polyacrylamide Gel , Ferrets , Freezing , Humans , Mice , Molecular Weight , Protein Denaturation
17.
GFP imaging in fixed cells.
Nybo, Kristie.
Biotechniques ; 52(6): 359-60, 2012 Jun.
Article in English | MEDLINE | ID: mdl-22668414

Subject(s)
Cytological Techniques/methods , Fixatives/chemistry , Green Fluorescent Proteins/chemistry , Aldehydes/chemistry , Antibodies/chemistry , Fluorescein-5-isothiocyanate , Fluorescent Dyes , Methanol/chemistry
18.
Non-specific antibody interactions.
Nybo, Kristie.
Biotechniques ; 52(5): 305-6, 2012 May.
Article in English | MEDLINE | ID: mdl-22578122

Subject(s)
Antibodies/chemistry , Immunoassay/methods , Immunohistochemistry/methods , Indicators and Reagents/chemistry , Animals , Antibodies/immunology , Antibody Affinity , Antibody Specificity , Biotin/chemistry , Immunoglobulin Fab Fragments/chemistry , Immunoglobulin Fab Fragments/immunology , Mice , Streptavidin/chemistry
19.
5' RACE.
Nybo, Kristie.
Biotechniques ; 52(3): 135-7, 2012 Mar.
Article in English | MEDLINE | ID: mdl-22401544

Subject(s)
DNA, Complementary/chemistry , Nucleic Acid Amplification Techniques/methods , Animals , DNA/chemistry , DNA/metabolism , DNA, Complementary/metabolism , Dogs , RNA/chemistry , RNA/metabolism , Reverse Transcriptase Polymerase Chain Reaction/methods , Ribonuclease H/metabolism , Sensitivity and Specificity , Skin/chemistry
20.
Cloning splice variants.
Nybo, Kristie.
Biotechniques ; 52(2): 77-9, 2012 Feb.
Article in English | MEDLINE | ID: mdl-22313399

Subject(s)
Alternative Splicing , Cloning, Molecular/methods , Protein Isoforms/genetics , Mutagenesis, Site-Directed/methods , Polymerase Chain Reaction/methods
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