ABSTRACT
Biolistic transmission of mRNA provides transient gene therapy to in vivo organs. This study documents particle mediated mRNA transmission to a solid organ and wound healing model using the mRNA of Green Fluorescent Protein to determine optimal delivery parameters. Renal function, bullet penetration, cellular injury, and Green Fluorescent Protein synthesis were quantified. Chimeric human epidermal growth factor-FLAG epitope cDNA or mRNA was transmitted to wounds in normal or steroid treated animals. Wound bursting strength, human epidermal growth factor-FLAG, and collagen synthesis were determined. Injury and bullet penetration correlated with the delivery velocity and bullet size. Optimal delivery parameters were established which provided widespread Green Fluorescent Protein synthesis. Human epidermal growth factor-FLAG treatment significantly increased collagen content and wound breaking strength in normal and steroid treated animals. FLAG protein synthesis was evident in mRNA treated fascia following treatment. We found the gene gun provides a novel method for efficient, in vivo delivery of mRNA-based therapeutic strategies to mammalian organs with minimal histologic damage allowing transient expression of protein in in vivo target tissues. Co-delivery of Green Fluorescent Protein mRNA may provide a useful positive control to determine effective transmission. Biolistic transmission of human epidermal growth factor-FLAG mRNA provides increased tissue epidermal growth factor levels and accelerates wound healing in normal and steroid exposed animals.
Subject(s)
Genetic Therapy/methods , Kidney/physiology , Luminescent Proteins , RNA, Messenger/pharmacology , Wounds and Injuries/therapy , Animals , Biological Availability , Disease Models, Animal , Drug Delivery Systems , Epidermal Growth Factor/pharmacology , Gene Transfer Techniques , Green Fluorescent Proteins , Humans , Immunohistochemistry , Male , Rats , Rats, Sprague-Dawley , Regeneration , Sensitivity and Specificity , Wound Healing/physiology , Wounds and Injuries/genetics , Wounds and Injuries/pathologyABSTRACT
Early coverage of deep burns of the elbow is vital to preserving the range of motion. Although various methods are used for coverage of this site, the brachioradialis muscle flap provides good coverage after debridement, with minimal donor site morbidity.
Subject(s)
Arm Injuries/surgery , Burns/surgery , Elbow Injuries , Surgical Flaps , Adult , Arm Injuries/etiology , Debridement , Humans , MaleABSTRACT
1. A ring embedded in the digits may result in erosion of the skin, tendons, and bones. 2. This type of injury may be prevented by removing the ring at the time of the injury. 3. All constricting bands should be carefully monitered and, if necessary, removed from fingers to prevent chronic erosion especially in people with a physical or mental disability.
