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1.
Pediatrics ; 96(3 Pt 2): 570-5, 1995 Sep.
Article in English | MEDLINE | ID: mdl-7659478

ABSTRACT

OBJECTIVE: To describe and evaluate the assays used to measure the antibody responses in infants to 13 experimental acellular pertussis vaccines and 2 licensed whole-cell pertussis vaccines. METHODS: During a 53-week period, preimmunization and postimmunization sera were assayed for immunoglobulin G antibodies to pertussis toxin, filamentous hemagglutinin, pertactin, and a mixture of type 2 and type 3 fimbriae by enzyme-linked immunosorbent assay (ELISA), for whole-cell agglutinins (AGG), and for pertussis toxin-neutralizing antibodies by the Chinese hamster ovary cell assay. All ELISA reagents were characterized to assure antigen and isotype specificity of the assays. Intralaboratory reproducibility and temporal stability were evaluated by analysis of results of control sera and by assessment of the response to the control whole-cell vaccine. Interlaboratory reproducibility was assessed by repeating the assays on preimmunization and postimmunization sera for 10% of the infants in a second laboratory. RESULTS: For control sera having antibody concentrations at least four times the minimum level of detection, the coefficients of variation within and between the ELISAs consistently were less than 20%. Trend analysis indicated that none of the assays drifted by more than 20% during the study period, and no significant drift was seen in the response to the control whole-cell vaccine. Results from the two laboratories correlated well; correlation coefficients were .93 or greater for the four ELISAs, .79 for the Chinese hamster ovary cell assay, and .82 for the AGG assay. For four of the six assays, there was either no difference or a modest (< 15%) difference in the geometric mean values for sera tested in both laboratories. Larger quantitative differences were observed for the AGG (45% difference) and pertactin (61% difference) assays. CONCLUSION: Assay reproducibility and stability indicate that the standardized methods can be transferred between laboratories, and that the results accrued during a 1-year period for the 15 vaccines can be compared.


Subject(s)
Antibodies, Bacterial/blood , Bordetella pertussis/immunology , Immunoassay/standards , Pertussis Vaccine/immunology , Agglutination Tests/standards , Animals , CHO Cells , Cricetinae , Diphtheria-Tetanus-Pertussis Vaccine/immunology , Enzyme-Linked Immunosorbent Assay/standards , Humans , Infant , Laboratories/standards , Pertussis Toxin , Reproducibility of Results , Virulence Factors, Bordetella/immunology
2.
Infect Immun ; 62(3): 1118-20, 1994 Mar.
Article in English | MEDLINE | ID: mdl-7509316

ABSTRACT

Elevated agglutinin titers have been shown to correlate with protection from disease following whole-cell pertussis vaccination, but the isotype and antigen specificity of human agglutinating antibodies is unknown. In 13 immunoassays, immunoglobulin G antifimbria antibodies had the strongest correlation with agglutinin titers following culture-proven infection with Bordetella pertussis (R' = 0.79; P < 0.0001) and following whole-cell pertussis vaccination (R' = 0.87, P < 0.0001).


Subject(s)
Agglutinins/analysis , Antibodies, Bacterial/analysis , Epitopes , Immunoglobulin Isotypes/analysis , Pertussis Vaccine/immunology , Whooping Cough/immunology , Enzyme-Linked Immunosorbent Assay , Humans , Vaccination
3.
Appl Environ Microbiol ; 38(5): 928-33, 1979 Nov.
Article in English | MEDLINE | ID: mdl-16345465

ABSTRACT

Bioluminescent bacteria were found in the water column, sediment, shrimp, and gastrointestinal tract of marine fishes from the semitropical estuarine environment of the East Lagoon, Galveston Island, Tex. Populations in the water column decreased during cold weather while sedimentary populations persisted. The highest percentages of luminous organisms were isolated from the gastrointestinal tract of marine fishes, where they persisted during 5 days of starvation. The presence of chitin temporarily increased intestinal populations. All isolates were Beneckea harveyi, whose natural habitat appears to be the gut of fishes and whose free-living reservoir appears to be marine sediments.

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