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1.
Article in English | MEDLINE | ID: mdl-35782533

ABSTRACT

One big challenge for undergraduate research students is gaining independence in the laboratory. In this curricular project, undergraduate students transformed research protocols developed for experienced scientists into protocols understandable to someone new to a laboratory. This process enabled themselves and other students to more quickly learn and master new techniques and advance to independent projects. Typically, students started with an original research protocol that assumed basic knowledge, such as instructions that came with a kit (i.e. plasmid purification kit instructions). Students created notes that explained the purpose of each step and reagent and provided example calculations. Then students illustrated the protocols with photos of materials needed, equipment used, action shots of difficult steps and screenshots of software programs. This approach has been used by students in laboratory courses and by new independent research students learning laboratory techniques. In the laboratory courses where students contributed to this project as part of a writing assignment, additional professional experience was gained by presenting a talk about their completed Illustrated Protocols to their classmates and by creating group posters that were presented at an undergraduate research symposium. After completion of this activity, undergraduate students gained confidence by applying their new knowledge to create user-friendly protocols. Students reported increased understanding of what is happening in each step, while instructors reported increased student independence and confidence that the protocol was being applied correctly and consistently. Thus, designing Illustrated Protocols enhanced learning and independence for the students creating the protocol and provided valuable help for future students.

2.
Cell Rep ; 38(5): 110323, 2022 02 01.
Article in English | MEDLINE | ID: mdl-35108532

ABSTRACT

Rhabdomyosarcoma (RMS) is a pediatric muscle sarcoma characterized by expression of the myogenic lineage transcription factors (TFs) MYOD1 and MYOG. Despite high expression of these TFs, RMS cells fail to terminally differentiate, suggesting the presence of factors that alter their functions. Here, we demonstrate that the developmental TF SIX1 is highly expressed in RMS and critical for maintaining a muscle progenitor-like state. SIX1 loss induces differentiation of RMS cells into myotube-like cells and impedes tumor growth in vivo. We show that SIX1 maintains the RMS undifferentiated state by controlling enhancer activity and MYOD1 occupancy at loci more permissive to tumor growth over muscle differentiation. Finally, we demonstrate that a gene signature derived from SIX1 loss correlates with differentiation status and predicts RMS progression in human disease. Our findings demonstrate a master regulatory role of SIX1 in repression of RMS differentiation via genome-wide alterations in MYOD1 and MYOG-mediated transcription.


Subject(s)
Homeodomain Proteins/metabolism , Muscle Development/genetics , Rhabdomyosarcoma/genetics , Transcription Factors/metabolism , Zebrafish Proteins/metabolism , Animals , Cell Differentiation/genetics , Gene Expression Regulation, Neoplastic/genetics , Mice , Muscle Development/physiology , MyoD Protein/metabolism , Myogenin/metabolism , Oncogene Proteins, Fusion/metabolism , Rhabdomyosarcoma/metabolism , Rhabdomyosarcoma, Embryonal , Zebrafish
3.
Dev Biol ; 407(2): 289-99, 2015 Nov 15.
Article in English | MEDLINE | ID: mdl-26256768

ABSTRACT

Neural crest cells (NCCs) are essential embryonic progenitor cells that are unique to vertebrates and form a remarkably complex and coordinated system of highly motile cells. Migration of NCCs occurs along specific pathways within the embryo in response to both environmental cues and cell-cell interactions within the neural crest population. Here, we demonstrate a novel role for the putative Sonic hedgehog (Shh) receptor and cell adhesion regulator, cdon, in zebrafish neural crest migration. cdon is expressed in developing premigratory NCCs but is downregulated once the cells become migratory. Knockdown of cdon results in aberrant migration of trunk NCCs: crestin positive cells can emigrate out of the neural tube but stall shortly after the initiation of migration. Live cell imaging analysis demonstrates reduced directedness of migration, increased velocity and mispositioned cell protrusions. In addition, transplantation analysis suggests that cdon is required cell-autonomously for directed NCC migration in the trunk. Interestingly, N-cadherin is mislocalized following cdon knockdown suggesting that the role of cdon in NCCs is to regulate N-cadherin localization. Our results reveal a novel role for cdon in zebrafish neural crest migration, and suggest a mechanism by which Cdon is required to localize N-cadherin to the cell membrane in migratory NCCs for directed migration.


Subject(s)
Cadherins/metabolism , Cell Adhesion Molecules/metabolism , Cell Movement , Neural Crest/cytology , Neural Crest/metabolism , Zebrafish Proteins/metabolism , Zebrafish/metabolism , Animals , Cell Adhesion Molecules/genetics , Cell Surface Extensions/metabolism , Embryo, Nonmammalian/metabolism , Gene Knockdown Techniques , Hedgehog Proteins/metabolism , Protein Transport , RNA, Messenger/genetics , RNA, Messenger/metabolism , Signal Transduction , Torso/embryology , Zebrafish Proteins/genetics
4.
J Cell Sci ; 127(Pt 10): 2291-301, 2014 May 15.
Article in English | MEDLINE | ID: mdl-24634509

ABSTRACT

Precise spatiotemporal regulation of the SIX1 homeoprotein is required to coordinate vital tissue development, including myogenesis. Whereas SIX1 is downregulated in most tissues following embryogenesis, it is re-expressed in numerous cancers, including tumors derived from muscle progenitors. Despite crucial roles in development and disease, the upstream regulation of SIX1 expression has remained elusive. Here, we identify the first direct mechanism for Six1 regulation in embryogenesis, through microRNA30a (miR30a)-mediated repression. In zebrafish somites, we show that miR30a and six1a and six1b (hereafter six1a/b) are expressed in an inverse temporal pattern. Overexpression of miR30a leads to a reduction in six1a/b levels, and results in increased apoptosis and altered somite morphology, which phenocopies six1a/b knockdown. Conversely, miR30a inhibition leads to increased Six1 expression and abnormal somite morphology, revealing a role for endogenous miR30a as a muscle-specific miRNA (myomiR). Importantly, restoration of six1a in miR30a-overexpressing embryos restores proper myogenesis. These data demonstrate a new role for miR30a at a key node in the myogenic regulatory gene network through controlling Six1 expression.


Subject(s)
Homeodomain Proteins/genetics , Homeodomain Proteins/metabolism , MicroRNAs/genetics , MicroRNAs/metabolism , Muscle Development/physiology , Zebrafish Proteins/genetics , Zebrafish Proteins/metabolism , Animals , Cell Differentiation/physiology , Cell Proliferation/physiology , Gene Expression Regulation, Developmental , Gene Knockdown Techniques , Gene Regulatory Networks , Homeodomain Proteins/biosynthesis , MicroRNAs/biosynthesis , Zebrafish , Zebrafish Proteins/biosynthesis
5.
J Proteome Res ; 11(10): 4894-905, 2012 Oct 05.
Article in English | MEDLINE | ID: mdl-22897585

ABSTRACT

Breast cancer patients diagnosed within five years following pregnancy have increased metastasis and decreased survival. A hallmark of postpartum biology that may contribute to this poor prognosis is mammary gland involution, involving massive epithelial cell death and dramatic stromal remodeling. Previous studies show pro-tumorigenic properties of extracellular matrix (ECM) isolated from rodent mammary glands undergoing postpartum involution. More recent work demonstrates systemic ibuprofen treatment during involution decreases its tumor-promotional nature. Utilizing a proteomics approach, we identified relative differences in the composition of mammary ECM isolated from nulliparous rats and those undergoing postpartum involution, with and without ibuprofen treatment. GeLC-MS/MS experiments resulted in 20327 peptide identifications that mapped to 884 proteins with a <0.02% false discovery rate. Label-free quantification yielded several ECM differences between nulliparous and involuting glands related to collagen-fiber organization, cell motility and attachment, and cytokine regulation. Increases in known pro-tumorigenic ECM proteins osteopontin, tenascin-C, and laminin-α1 and pro-inflammatory proteins STAT3 and CD68 further identify candidate mediators of breast cancer progression specific to the involution window. With postpartum ibuprofen treatment, decreases in tenascin-C and three laminin chains were revealed. Our data suggest novel ECM mediators of breast cancer progression and demonstrate a protective influence of ibuprofen on mammary ECM composition.


Subject(s)
Cyclooxygenase Inhibitors/pharmacology , Extracellular Matrix/metabolism , Ibuprofen/pharmacology , Mammary Glands, Animal/metabolism , Postpartum Period/metabolism , Animals , Basement Membrane/metabolism , Cells, Cultured , Extracellular Matrix/drug effects , Extracellular Matrix Proteins/chemistry , Extracellular Matrix Proteins/isolation & purification , Extracellular Matrix Proteins/metabolism , Female , Laminin/chemistry , Laminin/isolation & purification , Laminin/metabolism , Mammary Glands, Animal/drug effects , Mammary Glands, Animal/physiology , Postpartum Period/physiology , Protein Interaction Maps , Rats , Rats, Sprague-Dawley , Spectrometry, Mass, Electrospray Ionization , Tandem Mass Spectrometry
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