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1.
J Pharmacol Toxicol Methods ; 120: 107253, 2023.
Article in English | MEDLINE | ID: mdl-36806737

ABSTRACT

The number of animals used in a nonhuman primate (NHP) in vivo QTc assessment conducted as part of the safety pharmacology (SP) studies on a potential new drug is relatively small (4-8 subjects). The number is much smaller than the number of healthy volunteers in a conventional thorough QT (TQT) study (40-60 volunteers). How is it possible that such small studies could offer an equivalent sensitivity in an integrated nonclinical and clinical cardiac repolarization risk assessment? This study provided the opportunity to empirically demonstrate in a large number of NHPs the performance of a nonclinical evaluation at a similar size to a TQT study. By contrasting an analysis mimicking the sampling and aggregation of QTc interval data in a manner which is TQT-like with a more conventional SP-like analysis it was demonstrated that the SP-like analysis was more sensitive. In prospective power calculations 80% power at p = 0.05 can be achieved for a 5 ms QTc change with only n = 8 NHPs using the SP-like analysis and in a group of only 4 NHPs 80% power to detect 10 ms could be achieved. By contrast groups of 24 NHPs would be required to achieve 80% power to detect 5 ms using the TQT-like sampling and aggregation approach. Overall, this study has demonstrated that smaller safety pharmacology in vivo QTc assessments using all the available data in larger data aggregates can achieve sensitivity comparable to a human TQT study.


Subject(s)
Electrocardiography , Long QT Syndrome , Animals , Humans , Prospective Studies , Healthy Volunteers , Long QT Syndrome/chemically induced , Long QT Syndrome/diagnosis , Primates , Dose-Response Relationship, Drug , Heart Rate
2.
J Pharmacol Toxicol Methods ; 81: 144-50, 2016.
Article in English | MEDLINE | ID: mdl-26957141

ABSTRACT

INTRODUCTION: We describe experience with an unexpectedly high background incidence of atrioventricular block (AVB) observed in Mauritian cynomolgus monkeys (MCM) during preclinical safety assessment for bitopertin, a glycine-transporter-1 inhibitor. METHODS: Preclinical ECGs were reviewed to assess potential effects on cardiac conductivity, specifically AVB. RESULTS: Bitopertin administration in Chinese/Vietnamese monkeys (CVM; n=46) or MCM (n=64, from all relevant studies) revealed dose-dependent hypoactivity with a lack of expected increases in heart rate in response to chair-restraint during ECG recordings. Instances of 2° AVB were detected post-dose in two repeat-dose studies in MCM. AVB was generally restricted to animals showing a lower than expected increase in heart rate during restraint compared to placebo conditions (111-161 to 220-250bpm). A subsequent study in MCM prescreened for AVB found pre-existing 2° AVB in 15.4% of animals. After exclusion of these animals, no incidences of AVB were identified over 36weeks of bitopertin treatment. No evidence of AVB was observed in CVM in a 14-day study with continuous ECG recordings or in any clinical studies to date. DISCUSSION: Bitopertin-treatment was not associated with a direct effect on AV conduction in AVB naive MCM. Pre-test detection of AVB in MCM was likely due to the unmasking of pre-existing AVB through a slowed heart rate. The background incidence of AVB in the current MCM cohort was much higher than has been previously reported. These data suggest that ECG prescreening of unrestrained, nonstressed animals is recommended for the accurate assessment of possible treatment-related increases in AVB, especially in MCM.


Subject(s)
Arrhythmias, Cardiac/chemically induced , Arrhythmias, Cardiac/physiopathology , Cardiovascular Diseases/chemically induced , Cardiovascular Diseases/physiopathology , Macaca fascicularis/physiology , Animals , Atrioventricular Block/chemically induced , Atrioventricular Block/physiopathology , Dose-Response Relationship, Drug , Electrocardiography/drug effects , Female , Glycine Plasma Membrane Transport Proteins/antagonists & inhibitors , Heart Conduction System/drug effects , Heart Rate/drug effects , Male , Piperazines/pharmacology , Reproducibility of Results , Restraint, Physical , Safety , Sulfones/pharmacology , Telemetry
3.
J Pharmacol Toxicol Methods ; 69(3): 223-8, 2014.
Article in English | MEDLINE | ID: mdl-24508734

ABSTRACT

INTRODUCTION: Contemporary best practice recommendations in preclinical cardiovascular safety assessment promote 3Rs principles. This includes the employment of within-subjects experimental designs to evaluate discrete, acute doses of investigational new drugs, as well as the maintenance of stock colonies of appropriate large animal test systems. Such colony species are often tested repeatedly on independent studies with provision of appropriate recovery periods and requisite health status evaluations (e.g., physical examinations, electrocardiographic assessments, clinical pathology evaluations). METHODS: To investigate the utility of the often reiterative process of pre- or inter-study clinical pathology testing to help ascertain health status of non-naïve, telemetered canines (beagle dogs), the present study collated the results of a randomly selected set of animals approximately every three months for a period of three years. RESULTS: Although occasionally a few routine hematology or clinical chemistry endpoints did demonstrate evidence of systematic trending over time, none of the observed fluctuations fell outside the range of expected biological variability, nor would have prevented assignment of any given animal to study. DISCUSSION: The present findings illustrate a high degree of consistency in routinely assessed clinical pathology parameters during the course of chronic telemetry instrumentation in the canine, including relative to historical control data in healthy, experimentally naïve animals of the same species and source, maintained under analogous laboratory conditions. The data suggest that routine assessment of such parameters for the purposes of facilitating judgments concerning suitability for study may represent a pursuit of little overall value, and which may be reasonably accomplished based on alternative, observation-based screening procedures.


Subject(s)
Models, Animal , Pathology, Clinical/methods , Telemetry/methods , Animals , Biomarkers/metabolism , Dogs , Female , Health Status , Hematologic Tests/methods , Male , Telemetry/instrumentation
4.
J Pharmacol Toxicol Methods ; 69(2): 167-76, 2014.
Article in English | MEDLINE | ID: mdl-24262389

ABSTRACT

INTRODUCTION: Utilization of implantable bio-telemetry devices represents a common approach to contemporary cardiovascular safety assessment. Depending on the specific needs of the study design, and corresponding surgical methodologies employed, application of telemetry devices may have more or less liability to interact with ongoing physiology. The potential for intrathoracic procedures (epicardial/intracardiac ECG lead arrangements, left ventricular catheterization) to influence baseline cardiovascular function, and particularly arrhythmia status is currently an important topic of consideration. METHODS: Two experiments were performed to assess the post-surgical incidence of ventricular arrhythmias in cynomolgus monkeys instrumented with telemetry devices with 1) left ventricular pressure (LVP) transducers and epicardial lead array (N=67), and 2) epicardial lead array without LVP catheter placement (N=55). A third experiment (N=18) was performed to prospectively, and definitively, investigate the effect of chronic left ventricular catheterization on the observed incidences of arrhythmias by means of multiple (pre- and post-surgery) electrocardiographic evaluations conducted on ~24h of data per interval assessed up to ~12months post-implantation. RESULTS: The diversity and number of ventricular rhythm variants was considerably greater in animals instrumented with left ventricular catheters (62/67; 93%) compared to animals instrumented with epicardial leads only (21/55; 38.2%), and surgically naïve animals (9/18; 50%). Prior to surgery, the average frequency of all definitively characterized arrhythmias among experimentally naïve animals was 0.19/h; following surgical implantation of the telemetry device with epicardial leads and ventricular pressure catheter, the overall frequency of arrhythmia increased approximately 40-fold, to 7.19/h. DISCUSSION: Similar to prior investigations in canines, the present results confirm an increased incidence in the rate and variety of ventricular arrhythmias in cynomolgus monkeys when instrumented with telemetry devices equipped with LVP catheters. Instrumentation with epicardial leads was not associated with an increase in arrhythmias above that expected as a function of normal biological variation in experimentally naïve animals of this species.


Subject(s)
Arrhythmias, Cardiac/etiology , Catheters/adverse effects , Telemetry/adverse effects , Telemetry/instrumentation , Animals , Arrhythmias, Cardiac/physiopathology , Artifacts , Electrocardiography , Female , Macaca fascicularis , Male , Ventricular Pressure
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