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1.
Plant Physiol ; 124(3): 1105-20, 2000 Nov.
Article in English | MEDLINE | ID: mdl-11080288

ABSTRACT

By BLAST searching a large expressed sequence tag database for glutathione S-transferase (GST) sequences we have identified 25 soybean (Glycine max) and 42 maize (Zea mays) clones and obtained accurate full-length GST sequences. These clones probably represent the majority of members of the GST multigene family in these species. Plant GSTs are divided according to sequence similarity into three categories: types I, II, and III. Among these GSTs only the active site serine, as well as another serine and arginine in or near the "G-site" are conserved throughout. Type III GSTs have four conserved sequence patches mapping to distinct structural features. Expression analysis reveals the distribution of GSTs in different tissues and treatments: Maize GSTI is overall the most highly expressed in maize, whereas the previously unknown GmGST 8 is most abundant in soybean. Using DNA microarray analysis we observed increased expression among the type III GSTs after inducer treatment of maize shoots, with different genes responding to different treatments. Protein activity for a subset of GSTs varied widely with seven substrates, and any GST exhibiting greater than marginal activity with chloro-2,4 dinitrobenzene activity also exhibited significant activity with all other substrates, suggesting broad individual enzyme substrate specificity.


Subject(s)
Genes, Plant , Genome, Plant , Glutathione Transferase/genetics , Glycine max/genetics , Multigene Family , Zea mays/genetics , Cloning, Molecular , Models, Molecular , Phylogeny , Protein Structure, Quaternary , Sequence Alignment , Sequence Analysis, DNA , Glycine max/classification , Zea mays/classification
2.
Nat Biotechnol ; 18(2): 208-12, 2000 Feb.
Article in English | MEDLINE | ID: mdl-10657130

ABSTRACT

Isoflavones have drawn much attention because of their benefits to human health. These compounds, which are produced almost exclusively in legumes, have natural roles in plant defense and root nodulation. Isoflavone synthase catalyzes the first committed step of isoflavone biosynthesis, a branch of the phenylpropanoid pathway. To identify the gene encoding this enzyme, we used a yeast expression assay to screen soybean ESTs encoding cytochrome P450 proteins. We identified two soybean genes encoding isoflavone synthase, and used them to isolate homologous genes from other leguminous species including red clover, white clover, hairy vetch, mung bean, alfalfa, lentil, snow pea, and lupine, as well as from the nonleguminous sugarbeet. We expressed soybean isoflavone synthase in Arabidopsis thaliana, which led to production of the isoflavone genistein in this nonlegume plant. Identification of the isoflavone synthase gene should allow manipulation of the phenylpropanoid pathway for agronomic and nutritional purposes.


Subject(s)
Fabaceae/genetics , Flavanones , Genes, Plant , Isoflavones/metabolism , Oxygenases/genetics , Plants, Medicinal , Anthocyanins/biosynthesis , Arabidopsis/enzymology , Arabidopsis/genetics , Chenopodiaceae/enzymology , Chenopodiaceae/genetics , Cytochrome P-450 Enzyme System/genetics , Cytochrome P-450 Enzyme System/metabolism , Fabaceae/enzymology , Flavonoids/metabolism , Genetic Vectors , Genistein/metabolism , Genomic Library , Lignin/biosynthesis , Oxygenases/biosynthesis , Plants, Genetically Modified , Sequence Analysis, DNA , Glycine max/enzymology , Glycine max/genetics
4.
Drug Metabol Drug Interact ; 12(3-4): 171-87, 1995.
Article in English | MEDLINE | ID: mdl-8820851

ABSTRACT

Cytochromes P450 from higher plants share many general characteristics with those from animals and microorganisms. There are now 20 known P450 gene families in plants, with the number rapidly increasing. Many of these enzymes catalyze reactions in the secondary metabolic pathways of higher plants. The sheer number of plant species and the variety of these many pathways together result in the diverse enzyme chemistry available from P450s in the plant kingdom. Highlights of recent findings and of the contents of this journal issue are summarized.


Subject(s)
Cytochrome P-450 Enzyme System/metabolism , Plants/enzymology , Cytochrome P-450 Enzyme System/biosynthesis
5.
Drug Metabol Drug Interact ; 12(3-4): 383-9, 1995.
Article in English | MEDLINE | ID: mdl-8820863

ABSTRACT

Higher plant species, particularly tobacco, have been used as hosts for expression of both eucaryotic and procaryotic cytochromes P450. Strategies for subcellular localization and for providing access to a source of reducing equivalents have varied. While expression of the P450 gene can be confirmed by the appearance of mRNA or antigen, recovery of activity for in vitro studies is very difficult. Some plant-expressed heterologous cytochromes P450 dramatically alter the phenotype of the plant, so in vivo activity such as premature senescence or P450-specific alterations in herbicide sensitivity confirm that expression of a functional protein has occurred.


Subject(s)
Cytochrome P-450 Enzyme System/biosynthesis , Cytochrome P-450 Enzyme System/genetics , Plants/enzymology , Plants/genetics , Cytochrome Reductases/biosynthesis , Eukaryotic Cells/enzymology , Organelles/enzymology
6.
Biochim Biophys Acta ; 1201(1): 94-100, 1994 Sep 28.
Article in English | MEDLINE | ID: mdl-7918588

ABSTRACT

A cytochrome P-450 present in ripening avocado (Persea americana) fruit mesocarp (CYP71A1) had previously been shown to metabolize the monoterpenoids nerol and geraniol (Hallahan et al. (1992) Plant Physiol. 98, 1290-1297). Using DNA encoding CYP71A1 as a hybridization probe, we have shown by Southern analysis that a related gene is present in the catmint, Nepeta racemosa. RNA blot analysis, together with Western analysis of catmint leaf polypeptides using avocado cyt P-450 antiserum, showed that a closely related gene is expressed in catmint leaves. Cytochrome P-450 in catmint microsomes catalysed the specific hydroxylation of nerol and geraniol at C-10, whereas avocado CYP71A1, in either avocado microsomes or heterologously expressed in yeast, catalysed 2,3- or 6,7-epoxidation of these substrates. These results suggest that orthologous genes of the CYP71 family are expressed in these two plant species, but catalyse dissimilar reactions with monoterpenoid substrates.


Subject(s)
Cytochrome P-450 Enzyme System/metabolism , Plants/genetics , Terpenes/metabolism , Acyclic Monoterpenes , Gas Chromatography-Mass Spectrometry , Oxidation-Reduction , Plants/enzymology
7.
Plant Physiol ; 105(2): 473-482, 1994 Jun.
Article in English | MEDLINE | ID: mdl-12232216

ABSTRACT

The Streptomyces griseolus gene encoding herbicide-metabolizing cytochrome P450SU1 (CYP105A1) was expressed in transgenic tobacco (Nicotiana tabacum). Because this P450 can be reduced by plant chloroplast ferredoxin in vitro, chloroplast-targeted and nontargeted expression were compared. Whereas P450SU1 antigen was found in the transgenic plants regardless of the targeting, only those with chloroplast-directed enzyme performed P450SU1-mediated N-dealkylation of the sulfonylurea 2-methylethyl-2,3-dihydro-N-[(4,6-dimethoxypyrimidin-2-yl)aminocarbonyl]-1, 2-benzoisothiazole- 7-sulfonamide-1,1-dioxide (R7402). Chloroplast targeting appears to be essential for the bacterial P450 to function in the plant. Because the R7402 metabolite has greater phytotoxicity than R7402 itself, plants bearing active P450SU1 are susceptible to injury from R7402 treatment that is harmless to plants without P450SU1. Thus, P450SU1 expression and R7402 treatment can be used as a negative selection system in plants. Furthermore, expression of P450SU1 from a tissue-specific promoter can sequester production of the phytotoxic R7402 metabolite to a single plant tissue. In tobacco expressing P450SU1 from a tapetum-specific promoter, treatment of immature flower buds with R7402 caused dramatically lowered pollen viability. Such treatment could be the basis for a chemical hybridizing agent.

9.
Biochemistry ; 32(8): 1945-50, 1993 Mar 02.
Article in English | MEDLINE | ID: mdl-8448153

ABSTRACT

A cytochrome P450 with low affinity (2.9 x 10(3) M-1) for CO appears to be the major microsomal P450 in some plant tissues. The presence of low CO affinity cytochrome P450 correlates with its lack of NADPH reducibility and with the presence of high levels of 13(S)-hydroperoxy-9(Z),11(E)-octadecadienoate peroxidase activity. This activity and low CO affinity are retained by purified tulip cytochrome P450, which appears to be catalytically identical to a flaxseed-derived fatty acid allene oxide synthase P450 described previously [Song, W.-C., & Brash, A.R. (1991) Science 253, 781-784]. Other heme-binding ligands, such as CN- and imidazoles, bind weakly to the allene oxide synthase P450s, suggesting that axial coordination in the heme distal pocket may be hindered. We conclude that low CO affinity is characteristic of the allene oxide synthase P450s and that these P450s constitute a major portion of the microsomal P450 in a variety of plant tissues, particularly from monocot species.


Subject(s)
Carbon Monoxide/metabolism , Cytochrome P-450 Enzyme System/metabolism , Intramolecular Oxidoreductases , Isomerases/metabolism , Plants/enzymology , Cytochrome Reductases/metabolism , Kinetics , Oxidation-Reduction , Species Specificity
10.
Plant Physiol ; 100(4): 1976-81, 1992 Dec.
Article in English | MEDLINE | ID: mdl-16653226

ABSTRACT

One of the mRNAs that accumulates during the ripening of avocado (Persea americana Mill. cv Hass) has been previously identified as a cytochrome P450 (P450) monooxygenase and the corresponding gene designated CYP71A1. In this report we demonstrate that during ripening the accumulation of antigenically detected CYP71A1 gene product (CYP71A1) correlates with increases in total P450 and two P450-dependent enzyme activities: para-chloro-N-methylaniline demethylase, and trans-cinnamic acid hydroxylase (tCAH). To determine whether both of these activities are derived from CYP71A1, we have expressed this protein in yeast (Saccharomyces cerevisiae) using a galactose-inducible yeast promoter. Following induction, the microsomal fraction of transformed yeast cells undergoes a large increase in P450 level, attributable almost exclusively to the plant CYP71A1 protein. These membranes exhibit NADPH-dependent para-chloro-N-methylaniline demethylase activity at a rate comparable to that in avocado microsomes but have no detectable tCAH. These results demonstrate both that the CYP71A1 protein is not a tCAH and that a plant P450 is fully functional upon heterologous expression in yeast. These findings also indicate that the heterologous P450 protein can interact with the yeast NADPH:P450 reductase to produce a functional complex.

12.
Mol Microbiol ; 5(9): 2099-105, 1991 Sep.
Article in English | MEDLINE | ID: mdl-1766383

ABSTRACT

Many species within the order Actinomycetales contain one or more soluble cytochrome P450 monooxygenases, often substrate-inducible and responsible for a variety of xenobiotic transformations. The individual cytochromes exhibit a relatively broad substrate specificity, and some strains have the capacity to synthesize large amounts of the protein(s) to compensate for low catalytic turnover with some substrates. All three of the Streptomyces cytochromes sequenced to date are exclusive members of one P450 family, CYP105. In several instances, monooxygenase activity arises from induction of a P450 and associated ferredoxin, or of a P450 only, suggesting that some essential electron donor proteins (reductase and ferredoxin) are not co-ordinately regulated with the cytochrome. The overall properties of these systems suggest an adaptive strategy whose twofold purpose is to maintain a competitive advantage via the production of secondary metabolites, and, whenever possible, to utilize unusual growth substrates by introducing metabolites from these reactions into the more substrate-specific primary metabolic pathways.


Subject(s)
Actinomyces/enzymology , Cytochrome P-450 Enzyme System/physiology , Oxygenases/physiology , Amino Acid Sequence , Anti-Bacterial Agents/biosynthesis , Camphor 5-Monooxygenase , Electron Transport , Environmental Pollutants/metabolism , Mixed Function Oxygenases/physiology , Molecular Sequence Data , Pharmaceutical Preparations/metabolism , Sequence Homology, Nucleic Acid , Xenobiotics
13.
Mol Gen Genet ; 227(2): 238-44, 1991 Jun.
Article in English | MEDLINE | ID: mdl-2062304

ABSTRACT

Metabolism of sulfonylurea herbicides by Streptomyces griseolus ATCC 11796 is carried out via two cytochromes P-450, P-450SU1 and P-450SU2. Mutants of S. griseolus, selected by their reduced ability to metabolize a fluorescent sulfonylurea, do not synthesize cytochrome P-450SU1 when grown in the presence of sulfonylureas. Genetic evidence indicated that this phenotype was the result of a deletion of greater than 15 kb of DNA, including the structural genes for cytochrome P-450SU1 and an associated ferredoxin Fd-1 (suaC and suaB, respectively). In the absence of this monooxygenase system, the mutants described here respond to the presence of sulfonylureas or phenobarbital in the growth medium with the expression of only the subC,B gene products (cytochrome P-450SU2 and Fd-2), previously observed only as minor components in wild-type cells treated with sulfonylurea. These strains have enabled an analysis of sulfonylurea metabolism mediated by cytochrome P-450SU2 in the absence of P-450SU1, yielding an in vivo delineation of the roles of the two different cytochrome P-450 systems in herbicide metabolism by S. griseolus.


Subject(s)
Cytochrome P-450 Enzyme System/metabolism , Herbicides/metabolism , Streptomyces/metabolism , Sulfonylurea Compounds/metabolism , Blotting, Southern , Chromatography, High Pressure Liquid , Chromosome Deletion , Cytochrome P-450 Enzyme System/genetics , Ferredoxins/metabolism , Kinetics , Mutation , Streptomyces/genetics , Streptomyces/isolation & purification
14.
Biochemistry ; 30(2): 447-55, 1991 Jan 15.
Article in English | MEDLINE | ID: mdl-1846297

ABSTRACT

We have purified and characterized two ferredoxins, designated Fd-1 and Fd-2, from the soluble protein fraction of sulfonylurea herbicide induced Streptomyces griseolus. These cells have previously been shown to contain two inducible cytochromes P-450, P-450SU1 (CYP105A1) and P-450SU2 (CYP105B1), responsible for herbicide metabolism [O'Keefe, D. P., Romesser, J. A., & Leto, K. J. (1988) Arch. Microbiol. 149, 406-412]. Although Fd-2 is more effective, either ferredoxin can restore sulfonylurea monooxygenase activity to an aerobic mixture of NADPH, spinach ferredoxin:NADP oxidoreductase, purified cytochrome P-450SU1, and herbicide substrate. The gene for Fd-1 is located in the genome just downstream of the gene for cytochrome P-450SU1; the gene for Fd-2 follows the gene for P-450SU2. The deduced amino acid sequences of the two ferredoxins show that, if monomeric, each has a molecular mass of approximately 7 kDa, and alignment of the two sequences demonstrates that they are approximately 52% positionally identical. The spectroscopic properties and iron and acid-labile sulfide contents of both ferredoxins suggest that, as isolated, each contains a single [3Fe-4S] cluster. The presence of only three cysteines in Fd-1 and comparisons with three [4Fe-4S] ferredoxins with high sequence similarity suggest that both Fd-1 and Fd-2 have an alanine in the position where these [4Fe-4S] proteins have a fourth cysteine ligand to the cluster. Transformation of Streptomyces lividans, a strain unable to metabolize sulfonylureas, with DNA encoding both P-450SU1 and Fd-1 results in cells capable of herbicide metabolism. S. lividans transformants encoding only cytochrome P-450SU1 do not metabolize herbicide.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Ferredoxins/isolation & purification , Streptomyces/enzymology , Sulfonylurea Compounds/pharmacology , Amino Acid Sequence , Base Sequence , Chromatography, High Pressure Liquid , Cytochrome P-450 Enzyme System/metabolism , DNA, Bacterial/genetics , Electron Spin Resonance Spectroscopy , Enzyme Induction/drug effects , Ferredoxins/genetics , Genes, Bacterial , Mixed Function Oxygenases/metabolism , Molecular Sequence Data , Molecular Weight , Spectrophotometry, Ultraviolet , Streptomyces/genetics , Sulfonylurea Compounds/metabolism
15.
J Bacteriol ; 172(6): 3335-45, 1990 Jun.
Article in English | MEDLINE | ID: mdl-2345149

ABSTRACT

Streptomyces griseolus ATCC 11796 contains two inducible, herbicide-metabolizing cytochromes P-450 previously designated P-450SU1 and P-450SU2 (P-450CVA1 and P-450CVB1, respectively, using nomenclature of Nebert et al. [D. W. Nebert, M. Adesnik, M. J. Coon, R. W. Estabrook, F. J. Gonzalez, F. P. Guengerich, I. C. Gunsalus, E. F. Johnson, B. Kemper, W. Levin, I. R. Phillips, R. Sato, and M. R. Waterman, DNA 6:1-11, 1987]). Using antibodies directed against cytochrome P-450SU1, its N-terminal amino acid sequence, and amino acid composition, we cloned the suaC gene encoding cytochrome P-450SU1. Similar information about the cytochrome P-450SU2 protein confirmed that a gene cloned by cross-hybridization to the suaC gene was the subC gene encoding cytochrome P-450SU2. The suaC and subC genes were expressed in Escherichia coli, DNA for both genes was sequenced, and the deduced amino acid sequences were compared with that of the well-characterized cytochrome P-450CAM from Pseudomonas putida. Both cytochromes P-450SU1 and P-450SU2 contain several regions of strong similarity with the amino acid sequence of P-450CAM, primarily in regions of the protein responsible for attachment and coordination of the heme prosthetic group.


Subject(s)
Cytochrome P-450 Enzyme System/genetics , Herbicides/pharmacology , Streptomyces/genetics , Sulfonylurea Compounds/pharmacology , Amino Acid Sequence , Base Sequence , Cloning, Molecular , DNA, Bacterial/analysis , Enzyme Induction , Molecular Sequence Data , Streptomyces/enzymology , Sulfonylurea Compounds/metabolism
16.
Biochemistry ; 29(24): 5665-71, 1990 Jun 19.
Article in English | MEDLINE | ID: mdl-1974461

ABSTRACT

Both the chaperonin- and MgATP-dependent reconstitution of unfolded ribulosebisphosphate carboxylase (Rubisco) and the uncoupled ATPase activity of chaperonin 60 (groEL) require ionic potassium. The spontaneous, chaperonin-independent reconstitution of Rubisco, observed at 15 but not at 25 degrees C, requires no K+ and is actually inhibited by chaperonin 60, with which the unfolded or partly folded Rubisco forms a stable binary complex. The chaperonin-dependent reconstitution of Rubisco involves the formation of a complex between chaperonin 60 and chaperonin 10 (groES). Formation of this complex almost completely inhibits the uncoupled ATPase activity of chaperonin 60. Furthermore, although the formation of the chaperonin 60-chaperonin 10 complex requires the presence of MgATP, hydrolysis of ATP may not be required, since complex formation occurs in the absence of K+. The interaction of chaperonin 60 with unfolded or partly folded Rubisco does not require MgATP, K+, or chaperonin 10. However, discharge of the complex of chaperonin 60-Rubisco, which leads to the formation of active Rubisco dimers, requires chaperonin 10 and a coupled, K(+)-dependent hydrolysis of ATP. We propose that a role of chaperonin 10 is to couple the K(+)-dependent hydrolysis of ATP to the release of the folded monomers of the target protein from chaperonin 60.


Subject(s)
Adenosine Triphosphate/pharmacology , Bacterial Proteins/metabolism , Escherichia coli/metabolism , Potassium/pharmacology , Proteins/metabolism , Ribulose-Bisphosphate Carboxylase/metabolism , Adenosine Triphosphatases/metabolism , Chaperonins , Escherichia coli/drug effects , Hydrolysis , Protein Conformation , Temperature
17.
J Bacteriol ; 171(4): 1781-7, 1989 Apr.
Article in English | MEDLINE | ID: mdl-2495263

ABSTRACT

A soybean flour-induced, soluble cytochrome P-450 (P-450soy) was purified 130-fold to homogeneity from Streptomyces griseus. Native cytochrome P-450soy is a single polypeptide, with a molecular weight of 47,500, in association with one ferriprotoporphyrin IX prosthetic group. Oxidized P-450soy exhibited visible absorption maxima at 394, 514, and 646 nm, characteristic of a high-spin cytochrome P-450. The CO-reduced difference spectrum of P-450soy had a Soret maximum at 448 nm. When reconstituted with spinach ferredoxin and spinach ferredoxin:NADP+ oxidoreductase, purified cytochrome P-450soy catalyzed the NADPH-dependent oxidation of the xenobiotic substrates precocene II and 7-ethoxycoumarin. In vitro proteolysis of cytochrome P-450soy generated a stable and catalytically active cytochrome P-450, designated P-450soy delta.


Subject(s)
Cytochrome P-450 Enzyme System/isolation & purification , Streptomyces griseus/enzymology , Amino Acids/analysis , Biotransformation , Blotting, Western , Cytochrome P-450 Enzyme System/metabolism , Enzyme Induction , Heme/analysis , Isoelectric Point , Molecular Weight , Peptide Mapping , Glycine max , Spectrum Analysis , Xenobiotics/metabolism
18.
Plant Physiol ; 89(4): 1141-9, 1989 Apr.
Article in English | MEDLINE | ID: mdl-16666677

ABSTRACT

The microsomal fraction from the mesocarp of avocado (Persea americana) is one of few identified rich sources of plant cytochrome P-450. Cytochrome P-450 from this tissue has been solubilized and purified. Enzymatic assays (p-chloro-N-methylaniline demethylase) and spectroscopic observations of substrate binding suggest a low spin form of the cytochrome, resembling that in the microsomal membrane, can be recovered. However, this preparation of native protein is a mixture of nearly equal proportions of two cytochrome P-450 polypeptides that have been resolved only under denaturing conditions. Overall similarities between these polypeptides include indistinguishable amino acid compositions, similar trypsin digest patterns, and cross reactivity with the same antibody. The amino terminal sequences of both polypeptides are identical, with the exception that one of them lacks a methionine residue at the amino terminus. This sequence exhibits some similarities with the membrane targeting signal found at the amino terminus of most mammalian cytochromes P-450.

19.
Photosynth Res ; 17(3): 189-216, 1988 Sep.
Article in English | MEDLINE | ID: mdl-24429768

ABSTRACT

The chloroplast cytochrome bf complex is an intrinsic multisubunit protein from the thylakoid membrane consisting of four polypeptides: cytochrome f, a two heme containing cytochrome b 6, the Rieske iron-sulfur protein, and a 17 kD polypeptide of undefined function. The complex functions in electron transfer between PSII and PSI, where most mechanisms suggest that the transfer of a single reducing equivalent from plastoquinol to plastocyanin results in the translocation of two protons across the membrane. Primary sequence analyses, dichroism studies, and functional considerations allow the construction of an approximate structural model of a monomeric complex, although some evidence exists for a dimeric structure. Resolution of the properties of the two cytochrome b 6 hemes has relied upon the availability of purified solubilized complex, while evidence in the thylakoid suggests the difference between the two hemes are not as great in situ. Such variability in the spectroscopic and electrochemical properties of the cytochrome b 6 is a major concern during the experimental use of the purified complex. There is a general consensus that the complex contains a plastoquinol oxidizing (Qz) site, although the evidence for a plastoquinone reduction (Qc) site, called for in most mechanistic hypotheses, is less substantive. Probably the most severe challenge to the so called Q-cycle mechanism comes from experimental observations made with cytochrome b 6 initially reduced, where proposed interpretations more closely resemble a b-cycle than a Q-cycle. Although functional during cyclic electron transfer, the role of the complex and its possible interaction with other proteins, has not been completely resolved.

20.
Biochem Biophys Res Commun ; 140(2): 650-9, 1986 Oct 30.
Article in English | MEDLINE | ID: mdl-3778474

ABSTRACT

Inducible cometabolism of several sulfonylurea herbicides by Streptomyces griseolus has been shown to occur by hydroxylation, O-dealkylation, or deesterification reactions. Only after growth of the bacterium in the presence of sulfonylurea did cell-free extracts exhibit NAD(P)H-dependent sulfonylurea metabolism. These extracts were shown to contain elevated levels of soluble cytochrome P-450 and exhibit sulfonylurea induced difference spectra consistent with binding of substrate to cytochrome(s) P-450. These results establish the presence of an inducible cytochrome P-450-dependent sulfonylurea metabolizing system in S. griseolus.


Subject(s)
Cytochrome P-450 Enzyme System/biosynthesis , Herbicides/metabolism , Streptomyces/enzymology , Sulfonylurea Compounds/metabolism , Chloramphenicol/pharmacology , Enzyme Induction , Soil Microbiology
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