Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 4 de 4
Filter
Add more filters










Database
Language
Publication year range
2.
Ann Oncol ; 14(4): 549-53, 2003 Apr.
Article in English | MEDLINE | ID: mdl-12649099

ABSTRACT

BACKGROUND: Management strategies for women carrying BRCA1 and 2 mutations are becoming clearer and predictive testing for a known family mutation is commonly undertaken. Implications for men are not as clear and they participate less frequently. PATIENTS AND METHODS: Twenty-six men from 10 extended families underwent predictive testing. Their motivation, reaction and outcome were studied. Subjects had appropriate pre- and post-test counselling. Informed consent was obtained before predictive testing for known deleterious mutations. DNA analysis followed standard procedures. RESULTS: Eighteen tested positive and eight negative. Four had adverse psychological reactions and three reneged on their commitments to impart results. The spouse of another man had an adverse psychological reaction to the disclosure of his positive result. Two, already suffering from prostate cancer, were phenocopies and paternal lineage transmission was unexpectedly determined in another. Risk was removed from 33 offspring and confirmed for 56. CONCLUSIONS: Complex themes associated with genetic testing are confirmed and the spectrum extended. Men appear to understand the importance of participating in this process. Methods of avoiding adverse reactions merit further study along with other aspects of the process.


Subject(s)
Breast Neoplasms, Male/genetics , Genes, BRCA1 , Genes, BRCA2 , Genetic Counseling , Genetic Testing , Stress, Psychological , Adult , Aged , DNA Mutational Analysis , Family Health , Female , Humans , Male , Middle Aged , Patient Care Planning , Pedigree , Predictive Value of Tests , Prognosis
3.
Dis Aquat Organ ; 39(2): 109-19, 2000 Jan 14.
Article in English | MEDLINE | ID: mdl-10715816

ABSTRACT

The current taxonomy of Aeromonas salmonicida includes 4 subspecies. A. salmonicida subsp. salmonicida is associated with salmonid furunculosis, and A. salmonicida subsp. achromogenes, A. salmonicida subsp. masoucida, and A. salmonicida subsp. smithia are strains that show variation in some biochemical properties. This classification does not readily encompass isolates from a wide range of fish hosts currently described as atypical A. salmonicida. This study examined 17 typical strains, 39 atypical strains and 3 type A. salmonicida subspecies strains for genetic similarity using the random amplified polymophic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) techniques. On the basis of RAPD- and PFGE-derived profiles, similarity matrices and dendrograms were constructed. The results showed that species A. salmonicida constituted a genetically heterogeneous group of strains, encompassing within an homogeneous or clonal lineage comprised solely of typical strains and the A. salmonicida subsp. salmonicida type strain.


Subject(s)
Aeromonas/genetics , Genetic Variation , Electrophoresis, Gel, Pulsed-Field/veterinary , Genotype , Molecular Sequence Data , RNA, Ribosomal, 16S/chemistry , Random Amplified Polymorphic DNA Technique/veterinary
4.
Syst Appl Microbiol ; 21(1): 50-64, 1998 Mar.
Article in English | MEDLINE | ID: mdl-9741110

ABSTRACT

Fifty two isolates of atypical Aeromonas salmonicida, recovered from a wide range of hosts and geographical locations, were heterogeneous in terms of molecular and phenotypic characteristics, and represented taxa which could not be accommodated by the current classification of four subspecies. Generally, there was incongruence between the molecular (PCR, RAPD and ribotyping) and phenotypic methods in terms of cluster membership. By PCR, 6 groups were described of which Group 1 encompassed 12 isolates including the type strain of A. salmonicida subsp. smithia. Group 2 accommodated 23 isolates including the reference cultures of subspecies achromogenes and masoucida. The named culture of Haemophilus piscium was recovered in Group 6. By ribotyping and RAPD, the reference cultures were recovered in separate groups. All methods pointed to the uniqueness of subspecies smithia. Most isolates contained 2-6 plasmids, of 2.3 to 150 kb in length. Nevertheless, all isolates possessed certain key characteristics, including Gram-negativity, and the absence of motility.


Subject(s)
Aeromonas/classification , Aeromonas/isolation & purification , Bacterial Typing Techniques , Fishes/microbiology , Aeromonas/pathogenicity , Animals , DNA, Bacterial/analysis , DNA, Ribosomal/analysis , Fish Diseases/microbiology , Gram-Negative Bacterial Infections/microbiology , Gram-Negative Bacterial Infections/veterinary , Polymerase Chain Reaction , Random Amplified Polymorphic DNA Technique , rRNA Operon
SELECTION OF CITATIONS
SEARCH DETAIL
...