ABSTRACT
We demonstrate a record bandwidth high energy supercontinuum source suitable for multispectral photoacoustic microscopy. The source has more than 150 nJ/10 nm bandwidth over a spectral range of 500 to 1600 nm. This performance is achieved using a carefully designed fiber taper with large-core input for improved power handling and small-core output that provides the desired spectral range of the supercontinuum source.
Subject(s)
Fiber Optic Technology/instrumentation , Image Enhancement/instrumentation , Lighting/instrumentation , Microscopy/instrumentation , Photoacoustic Techniques/instrumentation , Crystallization , Equipment Design , Equipment Failure Analysis , Reproducibility of Results , Sensitivity and SpecificityABSTRACT
A commercial hybrid quadrupole time-of-flight mass spectrometer has been modified for high-speed matrix-assisted laser desorption ionisation (MALDI) imaging using a short-pulse optical technology Nd:YVO(4) laser. The laser operating in frequency-tripled mode (lambda = 355 nm) is capable of delivering 1.5-ns pulses of energy at up to 8 microJ at 5-10 kHz and 3 microJ at 20 kHz. Experiments to improve beam homogeneity and reduce laser speckle by mechanical vibration of the fibre-optic laser delivery system are reported along with data from trial and tissue imaging experiments using the modified instrument. The laser appeared to yield best results for MALDI-MS imaging experiments when operating at repetition rates 5-10 kHz. Combining this with raster imaging allowed images of rat brain sections to be recorded in 37 min. Similarly, images of the distribution of peptides in "on-tissue" digest experiments from tumour tissues were recorded in 1 h and 30 min rather than the 8-h acquisition time previously used. A brief investigation of targeted protein analysis/imaging by multiple reaction monitoring experiments "on-tissue" is reported. A total of 26 transitions were recorded over a 3-s cycle time and images of abundant proteins were successfully recorded.
Subject(s)
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/instrumentation , Animals , Brain Chemistry , Humans , Lasers , Male , Neoplasms/chemistry , Proteins/analysis , Rats , Rats, WistarABSTRACT
Following the publication of the new British Standard for the measurement of light reflectance values (LRVs), Keith Oakes, technical director for commercial and industrial flooring manufacturer Gradus, examines the importance of visual contrast in healthcare building design.
