Subject(s)
Endothelium/drug effects , Erythrocytes/physiology , Intercellular Junctions/drug effects , Snakes , Venoms/pharmacology , Animals , Basement Membrane/drug effects , Basement Membrane/ultrastructure , Capillaries/drug effects , Capillaries/ultrastructure , Endothelium/ultrastructure , Erythrocytes/ultrastructure , Male , RatsABSTRACT
Four methods were compared for determining the setting rate of amalgams. The resultant order of preference as a standard method was compressive strength at 30 minutes after preparation of the specimen, the time to the last transverse cut on a cylindrical specimen with a "guillotine" blade and the time to nonfracture of a ball of a amalgam with a final set Gillmore needle. A mercury absorption method was unacceptable. Alloys labelled fast and standard setting could be separated and limits between fast and standard alloys have been examined for each method.
Subject(s)
Chemistry, Physical/methods , Dental Amalgam , Absorption , Hardness Tests , Mercury , Needles , Pressure , Stress, Mechanical , Time FactorsSubject(s)
Cholera/etiology , Enterotoxins , Animals , Cholera/immunology , Diarrhea/etiology , Intestinal Mucosa/metabolism , MiceABSTRACT
A method for the analysis of glycosphingolipids in mammalian erythrocyte membranes is described. It consists of ozonolysis and alkaline treatment of the crude lipid extract to obtain oligosaccharides from glycosphingolipids and then gas-liquid chromatography of trimethylsilyl derivatives of glycitols derived from the oligosaccharides. Typical gas-liquid chromatographic patterns of oligosaccharide components were obtained with various mammalian erythrocytes; these corresponded to the glycosphingolipid compositions. The analysis could be carried out on 10 ml of packed erythrocytes.