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[Gelatinase activity of protein fractions obtained by electrophoresis in polyacrylamide gel]. / Izuchenie zhelatinaznoi aktivnosti v belkovykh fraktsiiakh, poluchennykh élektroforezom v poliakrilamidnom gele.

Kovalev, L I; Khasigov, P Z; Rubachev, P G; Obel'chuk, L I; Grachev, S V; Kovaleva, M A; Shishkin, S S.

Vopr Med Khim ; 48(3): 259-63, 2002.

Article in Russian | MEDLINE | ID: mdl-12243083

ABSTRACT

The modification of a method of determination gelatinolytic activity in protein fractions obtained by one and two-dimensional polyacrylamide gel electrophoresis is presented. The presence of a fraction 115-125 kD among human proteins of cortex matter and glomerulus of kidney and also in myocardium having gelatinolytic activity is demonstrated.

Subject(s)

Gelatinases/analysis , Chemical Fractionation , Electrophoresis, Polyacrylamide Gel/methods , Gelatinases/chemistry , Gelatinases/isolation & purification , Humans , Kidney/chemistry , Molecular Weight , Myocardium/chemistry

[Changes in alpha-B-crystalline in heart transplants]. / Izmeneniia al'fa-B-kpistallina v transplantatakh serdtsa.

Kovalev, L I; Ershova, E S; Obel'chuk, L I; Tsomartova, D A; Khasigov, P Z; Severin, V V; Riaboshtanova, E I; Il'inskii, I M; Shishkin, S S.

Biull Eksp Biol Med ; 125(2): 187-9, 1998 Feb.

Article in Russian | MEDLINE | ID: mdl-9559134

Subject(s)

Crystallins/metabolism , Graft Rejection/metabolism , Heart Transplantation , Myocardium/metabolism , Amino Acid Sequence , Crystallins/chemistry , Electrophoresis, Gel, Two-Dimensional , Humans , Liver/metabolism

ABSTRACT

Subject(s)

Subject(s)

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