1.
Anal Biochem
; 222(1): 14-8, 1994 Oct.
Article
in English
| MEDLINE
| ID: mdl-7856839
ABSTRACT
The detection and characterization of the phosphatases responsible for the dephosphorylation of N-phosphoryl groups, such as phospholysine, in proteins has been frustrated in large measure by a lack of suitable substrates for the assay of these enzymes. Herein we describe the preparation of phospholysine-containing amino acid homo- and heteropolymers by chemical means, and their use in a simple, nonradioactive assay for phospholysine phosphatase activity.