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ABSTRACT

A rapid semiquantitative method for determining the beta-lactamase inducing potency of drugs was developed. Bacteria carrying a gene for inducible beta-lactamase expression were inoculated at a concentration of 10(8) CFU/ml into microtiter plates for determination of MICs, which were recorded after 4 h of incubation. A suitable chromogenic beta-lactamase substrate was then added, and after incubation for another 3 h colour changes were monitored.

Subject(s)

Anti-Bacterial Agents/pharmacology , Bacteria/enzymology , beta-Lactamases/biosynthesis , Bacteria/drug effects , Bacteria/genetics , Drug Resistance, Microbial , Enterobacteriaceae/drug effects , Enterobacteriaceae/enzymology , Enterobacteriaceae/genetics , Enzyme Induction/drug effects , Humans , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/enzymology , Pseudomonas aeruginosa/genetics , Staphylococcus aureus/drug effects , Staphylococcus aureus/enzymology , Staphylococcus aureus/genetics , beta-Lactamases/genetics , beta-Lactams

ABSTRACT

Subject(s)

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