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Bioorg Khim ; 20(8-9): 919-31, 1994.
Article in Russian | MEDLINE | ID: mdl-7530008

ABSTRACT

A new technique based on Alu-PCR amplification of hn-RNA is described for the extraction of human-specific transcribed sequences from a hybrid cell line. Arrayed library of hn-cDNA was constructed and characterized by sequencing about 80 individual clones. A high enrichment by human-specific sequences (about 95%) was demonstrated.


Subject(s)
Chromosomes, Human, Pair 19 , Genomic Library , Hybrid Cells , Transcription, Genetic , Animals , Base Sequence , Cell Line , Chromosome Mapping , Cricetinae , DNA Primers , Humans , Molecular Sequence Data , RNA/genetics , Repetitive Sequences, Nucleic Acid
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