ABSTRACT
To establish at which extent the micronuclei (MN) frequency is influenced by occupational exposure to ionizing radiation, peripheral blood samples collected from 2151 subjects of both genders, smokers and non-smokers were analyzed. Among them, 378 (17.6%) were unexposed while the other 1773 (82.4%) were exposed to ionizing radiation for periods between 1 year and more than 30 years, at levels never overpassing legally regulated limits. The final results showed that for all investigated categories, MN frequency monotonously increases with the age by 1.75 ± 0.17 times in the case of exposed subjects, but independent on gender and smoking habit.
Subject(s)
Micronuclei, Chromosome-Defective , Occupational Exposure/analysis , Radiation, Ionizing , Adult , Female , Humans , Male , Micronucleus Tests , Risk Factors , Romania , SmokingABSTRACT
PURPOSE: In the framework of the 'Realizing the European Network of Biodosimetry' (RENEB) project, two intercomparison exercises were conducted to assess the suitability of an optimized version of the cytokinesis-block micronucleus assay, and to evaluate the capacity of a large laboratory network performing biodosimetry for radiation emergency triages. Twelve European institutions participated in the first exercise, and four non-RENEB labs were added in the second one. MATERIALS AND METHODS: Irradiated blood samples were shipped to participating labs, whose task was to culture these samples and provide a blind dose estimate. Micronucleus analysis was performed by automated, semi-automated and manual procedures. RESULTS: The dose estimates provided by network laboratories were in good agreement with true administered doses. The most accurate estimates were reported for low dose points (≤ 0.94 Gy). For higher dose points (≥ 2.7 Gy) a larger variation in estimates was observed, though in the second exercise the number of acceptable estimates increased satisfactorily. Higher accuracy was achieved with the semi-automated method. CONCLUSION: The results of the two exercises performed by our network demonstrate that the micronucleus assay is a useful tool for large-scale radiation emergencies, and can be successfully implemented within a large network of laboratories.
