ABSTRACT
Introducción: Los cannabinoides son vistos como agentes terapéuticos interesantes para la salud y la enfermería debido a sus efectos analgésicos, antieméticos y como relajantes musculares.Objetivo.Ver la opinión que poseen los estudiantes de Enfermería de la Universidad Mayor, sede de Temuco, acerca del uso terapéutico del cannabis.Sujetos y métodos.Es un estudio cualitativo, descriptivo y exploratorio. La muestra se conformó con 20 sujetos. Para la recogida de datos se utilizó la técnica de grupo focal y el análisis se hizo a través de la reducción de datos. Para la validez se utilizaron criterios de rigor, triangulando, además, por investigadores. El comité ético científico de la institución supervisó el aspecto ético.Resultados.Se identificaron 156 unidades de significado, que generaron cuatro categorías descriptivas: concepto de uso terapéutico, factores facilitadores, factores obstaculizadores e interés de los estudiantes. Se destacan de forma respectiva las más mencionadas: asociaciones de consumo de cannabis para la primera, en la que no hubo concepto como tal; respuesta analgésica para la segunda, estigmas sociales y ambiente legal para la tercera, y currículo y necesidad de conocer fórmulas farmacológicas para la última.Conclusión.Se evidencian conceptos débiles de uso terapéutico, y no se definió ni relacionó en términos farmacológicos o clínicos el concepto; factores facilitadores: acciones analgésicas potentes, efectos fisiológicos positivos sobre diversos sistemas corporales y cuidados paliativos como acción disciplinar; factores obstaculizadores: barreras y estigmas sociales, falta de información y poca apertura del marco legal; interés: incorporación al currículo, reconocer fórmulas farmacológicas y gestión del cuidado. (AU)
Introduction: Cannabinoids are seen as interesting therapeutic agents for health and Nursing due to their analgesic, antiemetic and muscle relaxant effects.Objective.To reveal the opinion held by nursing students at the University Mayor Temuco about the therapeutic use of Cannabis.Subjects and methods.It is a qualitative, descriptive, exploratory study; the sample consisted of 20 subjects, for the collection of data the focal group technique was used, the analysis through data reduction. Rigor criteria were used for validity, and researchers also triangulated. The ethical aspect was safeguarded by the Scientific Ethics Committee of the Institution.Results.156 units of meaning were identified, which generated four descriptive categories: therapeutic use concept, facilitating factors, hindering factors and interest of students. The most mentioned are highlighted: cannabis use associations for the first, where there was no concept as such; analgesic response for the second, social stigmas and legal environment for the third for the last curriculum and need to know pharmacological formulas.Conclusion.Weak concepts of 'therapeutic use' are evidenced, they did not define or relate in pharmacological or clinical terms the concept; facilitating factors: potent analgesic actions, positive physiological effects on various body systems, and palliative care emerges as a disciplinary action; obstacles: barriers and social stigmas, lack of information, little openness of the legal framework; interest: incorporate them in curriculum, recognize pharmacological formulas and management of care. (AU)
Subject(s)
Humans , Cannabis , Nursing , Students , Legislation, Drug , Receptors, Cannabinoid , Pharmaceutical PreparationsABSTRACT
Evidence accumulated so far indicates that circulating levels of microRNAs (miRNAs) are associated with several pathologies. Therefore, differential expression of extracellular miRNAs exhibits promising potential for screening and diagnosis purposes. We evaluated plasma miRNAs in response to the lipid-lowering drug atorvastatin in patients with hypercholesterolemia (HC) and controls. METHODS: We selected miRNAs based on previous data reported by our group and also by employing bioinformatics tools to identify 10 miRNAs related to cholesterol metabolism and statin response genes. Following miRNA identification, we determined plasma levels of miRNA-17-5p, miRNA-30c-5p, miRNA-24-3p, miRNA-33a-5p, miRNA-33b-5p, miRNA-29a-3p, miRNA-29b-3p, miRNA-454-3p, miRNA-590-3p and miRNA-27a-3p in 20 HC patients before and after 1 month of 20 mg/day atorvastatin treatment, evaluating the same miRNA set in a group of 20 healthy subjects, and employing qRT-PCR to determine differential miRNAs expression. RESULTS: HC individuals showed significant overexpression of miRNA-30c-5p and miRNA-29b-3p vs. NL (p = 0.0008 and p = 0.0001, respectively). Once cholesterol-lowering treatment was concluded, HC individuals showed a substantial increase of three extracellular miRNAs (miRNA-24-3p, miRNA-590, and miRNA-33b-5p), the latter elevated more than 37-fold (p = 0.0082). CONCLUSION: Data suggest that circulating miRNA-30c-5p and miRNA-29b-3p are associated with hypercholesterolemia. Also, atorvastatin induces a strong elevation of miRNA-33b-5p levels in HC individuals, which could indicate an important function that this miRNA may exert upon atorvastatin therapy. Additional studies are needed to clarify the role of this particular miRNA in statin treatment.
