ABSTRACT
This study assessed in vitro interaction between Bacillus bacteria and microalgae and their posterior in vivo effect on rearing Kumamoto oyster Crassostrea sikamea. The probiotic strains Bacillus licheniformis (MAt32), B. subtilis (MAt43) and B. subtilis (GAtB1) were individually inoculated in triplicate into 250 mL flasks containing 1 × 104 colony forming units (CFU) mL-1 of bacteria and 4.5 × 104 cell mL-1 of microalgae (Isochrysis galbana or Chaetoceros calcitrans) to evaluate their growth during a 7-day culture. Single cultures of microalgae or bacilli served as control. Additionally, C. sikamea spat was treated for 28 days with four single/combined bacillus treatments in triplicate at a concentration of 1 × 106 CFU mL-1 as follows: (a) control, without treatments; (b) combination of two antibiotics (10 mg L-1); (c) B. licheniformis; (d) B. subtilis; (e) B. subtilis subtilis and (f) mixed bacilli. The results showed a significantly (P < 0.05) increased growth of Bacillus strains co-cultured with microalgae, while the growth of I. galbana co-cultured with bacteria was not reduced significantly (P > 0.05) compared with the control group. C. sikamea spat treated with Bacillus showed significantly (P < 0.05) higher growth and survival than the control group. In this study, C. calcitrans microalgae were susceptible to the presence of probiotic bacteria. Nonetheless, this reduction in microalgal growth observed in vitro increased growth and survival of C. sikamea spat exposed to probiotic bacteria when compared to spat without probiotics.
Subject(s)
Bacillus , Crassostrea , Microalgae , Microbial Interactions , Probiotics , Animals , Bacillus/metabolism , Bacillus subtilis/metabolism , Crassostrea/growth & development , Crassostrea/microbiology , Haptophyta/metabolism , Microalgae/metabolism , Microbial Interactions/physiology , Probiotics/metabolismABSTRACT
En las unidades de cuidados criticos la utilizacion del cateter Swan Ganz permite monitorizar parametros hemodinamicos fundamentales para el control del paciente critico, para el diagnostico y tratamiento de aquellos que hemodinamicamente estan inestables, donde el cuidado de enfermeria es primordial no solo en los procedimientos de preparación de materiales a utilizar, asistencia en la colocación, sino tambien en el mantenimiento, cuidados y finalmente retirada del mismo..
