ABSTRACT
High-grade anal intraepithelial squamous lesion is significantly prevalent among men who have sex with men and are infected with the human immunodeficiency virus. This condition-the precursor to anal cancer-significantly increases the risk of developing it. Conversely, low-grade anal intraepithelial squamous typically follow a benign course and usually regress spontaneously. MATERIALS AND METHODS: To describe a population of men who have sex with men living with human immunodeficiency virus followed in a specialized anal cancer screening unit we conducted an observational, retrospective, and single-center study was. RESULTS: Ninety-four patients were analyzed, with a mean age of 39 ± 9 years, and a 87% positivity rate for high-risk human papillomavirus (HR-HPV). At the initial visit, 47% presented with low-grade squamous intraepithelial lesions. The progression rate to high-grade squamous intraepithelial lesion was 37.2 per 100,000 patients/year. None of the patients developed anal cancer. Tobacco and alcohol consumption were associated with this progression. DISCUSSION: In this series, longer duration of HIV infection, tobacco and alcohol use and the presence of HR-HPV were significantly associated with the occurrence of high-grade intraepithelial lesions. A lower risk of progression was seen in patients with higher education. CONCLUSION: In men who have sex with men living with HIV, the association of factors such as smoking, alcohol, the presence of HR-HPV and an increased burden of human papillomavirus disease makes these patients more susceptible to develop high-grade anal squamous lesions.
ABSTRACT
Over the past few years, venereal or sexually transmitted infections (STIs) have been on the rise worldwide requiring additional specialized monographic consultations to specifically treat STIs. Therefore, the Spanish Academy of Dermatology and Venereology (AEDV) Research Working Group on STIs and HIV has drafted this document with the necessary requirements in terms of infrastructure, personnel, technology, specific materials for sample collection, and needs for current therapeutic options. Strict emphasis is placed on the protection of patient privacy. A health care circuit model is outlined too. Additionally, a section has been included on contact tracking and reporting, key elements for the effective prevention and control of STIs. These clinical practice guidelines seek to establish a clinical action framework adapted to the current challenges posed by STIs and HIV in the dermatology, venereology, and multidisciplinary settings.
Subject(s)
Sexually Transmitted Diseases , Venereology , Sexually Transmitted Diseases/prevention & control , Sexually Transmitted Diseases/therapy , Humans , Venereology/standards , Patient Care Team , Spain , HIV Infections , Contact Tracing , Dermatology/standards , ConfidentialityABSTRACT
Introducción: El fibrosarcoma ovárico es un tumor maligno del estroma ovárico muy infrecuente, con pocos casos reportado en la literatura. Principales síntomas o hallazgos clínicos: Mujer de 56 años posmenopáusica, con sangrado vaginal escaso de varios días de evolución y dolor en fosa ilíaca derecha sin fiebre ni clínica digestiva. La exploración ginecológica era normal.Diagnósticos principales, intervenciones terapéuticas y resultados: La ecografía transvaginal mostró un endometrio homogéneo, un mioma de 2,4×1cm y una lesión sólido-quística heterogénea de tabiques gruesos de 6,4×6,8cm en el ovario izquierdo. El estudio histológico intraoperatorio se informó como: neoplasia sólida sospechosa de malignidad. Se le realizó histerectomía más doble anexectomía, omentectomía y linfadenectomía. Histológicamente la neoplasia estaba constituida por células fusiformes dispuestas en láminas y fascículos entrecruzados con apariencia difusa en espiga, con atipia nuclear moderada, áreas de necrosis y focos de hemorragia. Se identificaron 12 mitosis por 10 campos de gran aumento. Inmunohistoquímicamente las células fueron positivas para inhibina, actina 1A4, vimentina, calretinina, CD56 y CD99. El índice proliferativo con Ki-67 fue del 25%. El diagnosticó definitivo fue: fibrosarcoma primario de ovario. Un año después, la paciente se encontraba libre de enfermedad sin ninguna terapia adyuvante y continúa en seguimiento. Conclusión: El fibrosarcoma de ovario es una neoplasia maligna excepcional de mal pronóstico. En la actualidad, los tratamientos y factores pronósticos del fibrosarcoma ovárico siguen siendo discutibles. El presente caso destaca el importante papel del estudio intraoperatorio y la inmunohistoquímica para su correcto diagnóstico.(AU)
Introduction: Ovarian fibrosarcoma is an extremely rare malignant ovarian stromal tumor, with few cases reported in the literature. Main symptoms and/or clinical findings: A 56-year-old posmenopausal woman with scant vaginal bleeding of several days duration and pain in the right iliac fossa without fever or digestive symptoms. The gynecological examination was normal. Main diagnoses, therapeutic interventions and results: Transvaginal ultrasound showed a homogeneous endometrium, a 2.4×1cm myoma, and a heterogeneous solid-cystic lesion with thick septa measuring 6.4×6.8cm in the left ovary. An intraoperative histological study was performed, which was reported as: solid neoplasm suspicious of malignancy. Hysterectomy plus double adnexectomy, omentectomy, and lymphadenectomy were performed. Histologically, the neoplasm was made up of spindle cells arranged in sheets and intertwined fascicles with a diffuse spike-like appearance, with moderate nuclear atypia, areas of necrosis and hemorrhage foci; 12 mitoses were identified for every 10 high-power fields. Immunohistochemically the cells were positive for inhibin, actin 1A4, vimentin, calretinin, CD56 and CD99. The proliferative rate with Ki-67 was 25%. The definitive diagnosis was: primary ovarian fibrosarcoma. One year later, the patient is free of the disease without any adjuvant treatment and continues to be followed up. Conclusion: Ovarian fibrosarcoma is an exceptional malignancy with a poor prognosis. Currently, the treatments and prognostic factors for ovarian fibrosarcoma are still the subject of debate. This case highlights the important role of the intraoperative study and immunohistochemistry for its correct diagnosis.(AU)
Subject(s)
Humans , Female , Middle Aged , Fibrosarcoma/diagnostic imaging , Fibrosarcoma/etiology , Immunohistochemistry , Ovary , Gynecology , Inpatients , Physical Examination , Symptom Assessment , LaparoscopyABSTRACT
Introducción: El tumor uterino que recuerda al tumor de los cordones sexuales del ovario (UTROSCT) es un tumor muy infrecuente de histogénesis incierta, incluido en la actual clasificación de tumores del estroma endometrial de la Organización Mundial de la Salud. Principales síntomas y/o hallazgos clínicos: Mujer de 48 años, sin antecedentes de interés, acudió a consulta por hipermenorreas en los últimos ciclos. La exploración ginecológica era normal. Diagnósticos principales, intervenciones terapéuticas y resultados: El estudio ecográfico mostró una lesión nodular submucosa de 26×21mm. Se le realizó histeroscopia diagnóstica, identificándose un mioma submucoso no accesible para extirpación ambulatoria. Se llevó a cabo histeroscopia quirúrgica procediéndose a miomectomía con asa de diatermia. Histológicamente se observó tejido muscular liso masivamente infiltrado por células de poco citoplasma con diferenciación glandular positivas con citoqueratinas, interpretándose como fragmentos de pared miometrial infiltrados por carcinoma. Se le realizó estudio de extensión e histerectomía. En la pieza quirúrgica no se identificó neoplasia residual. Se revaluó la biopsia previa y se amplió el estudio inmunohistoquímico, observándose positividad para marcadores de los cordones sexuales, epiteliales y musculares lisos. Ante estos hallazgos, el diagnóstico definitivo fue UTROSCT. Conclusión: El UTROSCT muestra generalmente un comportamiento benigno. Sin embargo, se considera de potencial maligno incierto, debido a que presenta una baja tasa de recurrencias y metástasis excepcionales. Desde el punto de vista histopatológico es importante reconocer esta entidad, ya que su histológica es variada imitando una amplia gama de tumores tanto benignos como malignos, por lo que es necesario realizar estudio inmunohistoquímico para su correcto diagnóstico.(AU)
Introduction: Uterine tumour resembling ovarian sex cord tumour (UTROSCT) is a very rare tumour of uncertain histogenesis, included in the current classification of endometrial stromal tumours of the World Health Organization. Main symptoms and/or clinical findings: 48-year-old woman with no history of interest, consulted for hypermenorrhoea in recent cycles. The gynaecological examination was normal. Main diagnoses, therapeutic interventions, and results: The ultrasound study showed a submucosal nodular lesion of 26×21mm. A diagnostic hysteroscopy was performed, identifying a submucosal myoma not accessible for outpatient removal. Surgical hysteroscopy was performed, proceeding to myomectomy with loop diathermy. Histologically, smooth muscle tissue was observed massively infiltrated by cells with scant cytoplasm and positive glandular differentiation for cytokeratins, interpreted as fragments of the myometrial wall infiltrated by carcinoma. An extension study and hysterectomy were performed. No residual neoplasia was identified in the surgical piece. A previous biopsy was re-evaluated and an immunohistochemical study was extended, showing positivity for sexual cord, epithelial, and smooth muscle markers. Given these findings, the definitive diagnosis was UTROSCT. Conclusion: UTROSCT generally shows benign behaviour. However, it is considered to be of uncertain malignant potential, due to its low rate of recurrence and rare metastases. From the histopathological point of view, it is important to recognize this entity, since its histology is varied, mimicking a wide range of tumours, both benign and malignant, making it necessary to perform an immunohistochemical study for its correct diagnosis.(AU)
Subject(s)
Humans , Female , Middle Aged , Uterine Neoplasms , Uterus , Immunohistochemistry , Inpatients , Physical Examination , Neoplasms , GynecologyABSTRACT
Trinidad and Tobago, a neotropical country, has 38 reported chigger species. Of these species, 18 were parasitizing bats. Here, we describe a new genus and species parasitizing a ghost-faced bat in this country.
Subject(s)
Caves , Chiroptera , Trombiculiasis , Trombiculidae , Animals , Chiroptera/parasitology , Species Specificity , Trinidad and Tobago , Trombiculiasis/parasitology , Trombiculidae/classificationABSTRACT
Trinidad and Tobago, a neotropical country, has 38 reported chigger species. Of these species, 18 were parasitizing bats. Here, we describe a new genus and species parasitizing a ghost-faced bat in this country.
ABSTRACT
La variante mixoide del sarcoma del estroma endometrial es una neoplasia uterina extremadamente rara caracterizada por la presencia de predominante material mixoide y células fusiformes atípicas que muestran características histopatológicas e inmunohistoquímicas de sarcoma del estroma endometrial. Presentamos 2 casos clinicopatológicos de mujeres de 54 y 76 años de edad, ambas con metrorragia posmenopáusica, útero aumentado de tamaño e imagen de tumoración dependiente de la pared miometrial, sometidas a histerectomía total con doble anexectomía con diagnóstico anatomopatológico de sarcoma del estroma endometrial variante mixoide
Myxoid endometrial stromal sarcoma is an extremely rare uterine neoplasm characterised by the presence of atypical predominantly myxoid material fusiform cells showing histopathological and immunohistochemical characteristics of endometrial stromal sarcoma. We present two clinicopathological cases of a 54-year-old woman and 76-year-old woman, both with postmenopausal vaginal bleeding, enlarged uterus and myometrial wall-dependent tumour imaging, undergoing total hysterectomy with double oophorectomy with pathological diagnosis of myxoid endometrial stromal sarcoma
Subject(s)
Humans , Female , Middle Aged , Aged , Sarcoma, Endometrial Stromal/diagnosis , Biopsy , Leiomyoma/diagnosis , Leiomyoma/surgery , Sarcoma, Endometrial Stromal/pathology , Sarcoma, Endometrial Stromal/surgery , Leiomyosarcoma/pathology , Immunohistochemistry , Hysterectomy/methodsABSTRACT
We verified infestation of Oligonychus milleri (McGregor) on plantations of Pinus caribaea (Pinaceae) and of Oligonychus ununguis (Jacobi) on plantations of Eucalyptus urophylla x Eucalyptus grandis (Myrtaceae) in State of Rondônia, Northern region of Brazil. This represents the first record of O. milleri in Brazil. Oligonychus ununguis was recorded previously, on cypress. The damage caused by these two spider mites in the plantations is described herein.
Subject(s)
Eucalyptus , Pinus , Tetranychidae , Animals , BrazilABSTRACT
In the Neotropical region the genus Quadraseta Brennan, 1970, includes 14 species, with ectoparasitic habits during the larval stage. Quadraseta brasiliensis Goff and Gettinger, 1989, was described from larvae collected on the rodent Hylaeamys megacephalus (Fisher), cited as Oryzomys capito (Olfers). According to these authors, the holotype was deposited in the Museu de Zoologia da Universidade de Sao Paulo and the paratypes were deposited in three other collections: Bernice Pauahi Bishop Museum, Sam Noble Oklahoma Museum of Natural History and United States National Museum of Natural History, however, no type specimens were found in any of these museums. Here we redescribe the larva, describe the deutonymph instar obtained from field-collected larvae, and report new hosts and localities for this species in Brazil. In addition we provide sequences of the 18S ribosomal RNA gene for this species.
ABSTRACT
In the Neotropical region the genus Quadraseta Brennan, 1970, includes 14 species, with ectoparasitic habits during the larval stage. Quadraseta brasiliensis Goff and Gettinger, 1989, was described from larvae collected on the rodent Hylaeamys megacephalus (Fisher), cited as Oryzomys capito (Olfers). According to these authors, the holotype was deposited in the Museu de Zoologia da Universidade de Sao Paulo and the paratypes were deposited in three other collections: Bernice Pauahi Bishop Museum, Sam Noble Oklahoma Museum of Natural History and United States National Museum of Natural History, however, no type specimens were found in any of these museums. Here we redescribe the larva, describe the deutonymph instar obtained from field-collected larvae, and report new hosts and localities for this species in Brazil. In addition we provide sequences of the 18S ribosomal RNA gene for this species.
ABSTRACT
In patients with portal hypertension, ectopic varices can develop at any site along the gastrointestinal tract outside the classically described gastroesophageal location. Like esophageal variceal hemorrhage, bleeding from ectopic varices can be life-threatening. Diagnosis and treatment of ectopic varices can be challenging; to date, no effective treatment algorithm has been described. A systematic teamwork approach to diagnosing and treatment of ectopic varices is required to successfully manage hemorrhage from ectopic varices.
Subject(s)
Algorithms , Disease Management , Esophageal and Gastric Varices/therapy , Gastrointestinal Hemorrhage/therapy , Hypertension, Portal/complications , Esophageal and Gastric Varices/complications , Gastrointestinal Hemorrhage/etiology , Humans , Hypertension, Portal/therapy , Ligation , Male , Middle AgedABSTRACT
El objetivo del estudio fue comparar el índice de madurez placentaria y las alteraciones histopatológicas en placentas de recién nacidos/óbitos con y sin defecto congénito (DC). Se realizó un estudio tipo casos y controles. Se incluyeron n=25 casos yn=50 controles sin DC. Los casos se clasificaron de acuerdo a la presencia de DC aislado (n=17) omúltiple (n=8). Se incluyeron recién nacidos/óbitos (RN) con una edad gestacional (EG) > 20 semanas. Se excluyeron embarazos gemelares. Se recolectó información sobre las características clínicas del producto y de la madre, antropometría del RN y su evaluación de APGAR. Se examinó la placenta, macroscópica y microscópicamente, para determinar la presencia y extensión de alteraciones histopatológicas. Se determinó el índice de madurez placentaria (IMP), calculado dividiendo el número de membranas vásculo sinciciales en 1 mm2 entre el grosor de las mismas (µ). El IMP (media ±DE) fue de 27.77±14 en el grupo de controles, 30.31±12 en el grupo de casos aislados y 16.76± 1 en el grupo de DC múltiple (p<0.05).El resto de las variables evaluadas no mostraron diferencias significativas entre grupos. En conclusión este trabajo muestra una menor madurez placentaria asociada con la presencia de DC múltiple.
The objective of the study was to compare the placental maturity index (PMI) and pathohistological alterations of placentas from newborn/stillborns (NB) with or without congenital defects (CD). A case control study was carried out. N=25 CD cases and n=50 controls without CD were included. Cases were classified according to the presence of simple (n=17) or multiple (n=8) CD. Newborn/stillborns with gestational age >20 weeks were included. Twin pregnancies were excluded. We collected information on clinical characteristics of the NB and the mother, NB anthropometry and APGAR score. The placenta was macro and microscopically examined to determine the presence and extension of histological alterations. The PMI was obtained by dividing the number of vasculo-syncytial membranes in 1 mm2, by their thickness (µ). The PMI (Mean ± SD) was 27.77 ± 14 in the control group, 30.31 ± 12 in the group diagnosed with simple CD, and 16.76 ± 1 in the group diagnosed with multiple CD (p<0.05. The rest of the assessed variables did not show significant difference between groups. In conclusion, this work shows a lower placental maturity associated with the presence of multiple CD.
Subject(s)
Humans , Female , Pregnancy , Infant, Newborn , Congenital Abnormalities/epidemiology , Placenta/pathology , Case-Control StudiesABSTRACT
Introducción: El objetivo de este estudio es presentar los resultados del seguimiento durante 8 años de 54 casos de instrumentos rotos intraconducto, que no pudieron ser removidos y/o sobrepasados, y que fueron obturados con óxido de zinc-eugenol (ZOE) y formocresol. Material y métodos: Se incluyeron 54 pacientes con fractura de instrumentos en conductos radiculares de dientes posteriores, 23 no presentaban lesión periapical y 31 tenían lesión. Los instrumentos fracturados tenían longitudes de 2-4 mm en tercio apical y no pudieron ser removidos y/o sobrepasados con ninguna técnica. Los conductos fueron preparados al borde del instrumento fracturado, hasta un calibre 45 en dirección apical con técnica telescópica, irrigando con hipoclorito de sodio al 3% y desmineralizando con 0,25 ml de EDTA al 16%, lavados con 3 ml de solución fisiológica y secados con puntas de papel, se obturaron utilizando 0,01 ml de formocresol, 0,05 ml de eugenol mezclados con óxido de zinc y cono de gutapercha calibre 45 mediante condensación lateral. Los conductos restantes fueron instrumentados telescópicamente y obturados con gutapercha y ZOE mediante condensación lateral. Resultados: Se realizaron controles clínicos y radiográficos a uno, tres, cinco y ocho años. Al año, los casos oscilaron entre 83,9-95,7% asintomáticos y negativos a la percusión, fístula ausente entre 93,5-100% y sin lesión periapical de 32,3-91,3%. A tres años, 96,8-100% asintomáticos y negativos a la percusión, ausencia de fístula y sin lesión de 80,6-95,7%. A cinco años, 96,8-100% asintomáticos y negativos a la percusión, ausencia de fístula y 93,5- 100% sin lesión. A ocho años, 100% asintomáticos, negativos a percusión, ausencia de fistula y 93,5-95,7% sin lesión. Conclusiones: La utilización de ZOE-formocresol es una alternativa terapéutica exitosa en casos de instrumentos fracturados que no pudieron ser removidos y/o sobrepasados
Introduction: The aim of this study is to present the results of monitoring over 8 years of 54 cases of intracanal broken instruments, which could not be removed and/or bypassed, and were sealed with zinc oxide-eugenol (ZOE) and formocresol. Material and methods: Fifty-four patients with broken instruments in root canals of posterior teeth, 23 had no periapical lesion and 31 had periapical lesion. Fractured instruments were had lengths of 2-4 mm in apical third and were not removed and/or exceeded with any technique. Root canals were prepared at the edge of the fractured instrument using the telescope technique up to 45 file. Irrigation was performed with sodium hypochlorite 3% and 0.25 ml of 16% EDTA. Then, the root canals were washed with saline solution 3 ml and dried with paper points. Obturation was carried out using 0.01 ml formocresol plus 0.05 ml of eugenol mixed with zinc oxide and a guttapercha master cone number 45 using lateral condensation. Remaining canals were telescopically instrumented and filled with guttapercha and ZOE by lateral condensation. Results: Clinical and radiographic controls at one, three, five and eight years were performed. After one year, 83.9-95.7% were asymptomatic and negative to percussion, absent fistula between 93,5-100%, without periapical lesion 32,3-91,3%. After three years 96,8-100% asymptomatic and negative on percussion, absence of fistula, without periapical lesion 80,6-95,7%. At five years 96,8-100% asymptomatic and negative on percussion, absence of fistula and 93,5- 100% without periapical lesion. In eight years, 100% asymptomatic, negative percussion and absence of fistula and 93,5-95,7% without periapical lesion. Conclusions: The use of zinc oxide-eugenol and formocresol is a successful therapeutic option in cases of fractured instruments that could not be removed and/or exceeded
Subject(s)
Humans , Male , Female , Root Canal Obturation/instrumentation , Zinc Oxide-Eugenol Cement/therapeutic use , Formocresols/therapeutic use , Periprosthetic Fractures/diagnosis , Sodium Hypochlorite/therapeutic use , Tooth Demineralization , Dental Pulp Cavity/anatomy & histology , Periapical Diseases , Periodontal Diseases/diagnosis , Radiography, Dental/methodsABSTRACT
This study reports the percentage of cattle farms with ivermectin (IVM) resistant gastrointestinal nematodes (GINs) in Veracruz, Mexico, and identifies the GIN genera involved in the resistances. It also describes management practices of anthelmintic (AH) use on the surveyed farms. Twenty-one farms were assessed by means of the faecal egg count reduction test using the McMaster technique. Only two farms had GIN populations susceptible to IVM (9.5%). The proportion of farms with IVM resistant GIN was 71.4% (15/21). Seven of these farms had less than 80% egg count reductions. Haemonchus and Cooperia were the genera most commonly found in the resistant populations, followed by Oesophagostomum. Inappropriate AH treatment practices were identified from the completed questionnaires. Further management practices such as selective treatment and quarantine treatments are proposed to further reduce the spread of IVM resistance between farms.
Subject(s)
Cattle Diseases/parasitology , Drug Resistance , Gastrointestinal Diseases/veterinary , Ivermectin/pharmacology , Nematoda/drug effects , Nematode Infections/veterinary , Animals , Anthelmintics/pharmacology , Cattle , Cattle Diseases/epidemiology , Data Collection , Gastrointestinal Diseases/epidemiology , Gastrointestinal Diseases/parasitology , Mexico/epidemiology , Nematode Infections/epidemiology , Nematode Infections/parasitology , Surveys and QuestionnairesABSTRACT
La argirosis focal oral es una lesión yatrogénica benigna originada por la presencia en tejido conectivo subepitelial de fragmentos de amalgama durante un procedimiento operatorio. En este artículo se presenta el caso clínico de pigmentación en mucosa vestibular originada por utilizar amalgama como material obturador en una apicectomía en un incisivo lateral superior derecho. Una vez realizado el diagnóstico diferencial y valorar clínica y radiográficamente el caso, se optó por realizar una segunda intervención utilizando en la obturación a retro IRM. El procedimiento quirúrgico se realizó con el diseño de un colgajo de espesor total de Reinmoller, cureteando y adelgazando la cara interna del colgajo mucoperióstico a fin de disminuir al máximo El grado de pigmentación presentado y dándole seguimiento hasta 13 meses para evidenciar neoformación ósea, reducción de la lesión periapical y mejoría en el color de las mucosas
The oral focal argyrosis is a benign iatrogenic lesion caused by the presence in the subepithelial connective tissue of fragments amalgam during a surgical procedure. This article presents the case of pigmentation in vestibular mucosa caused by using amalgam as obturation material in an apicectomy in upper right lateral incisor. Once differential diagnosis and assess both clinically and radiographically case, opted for a second intervention using the IRM to retro. The surgical procedure was performed with a total thickness of Reinmoller flap design, curettage and slimming the inside in order to decrease mucoperiosteal flap to maximize the degree of pigmentation presented and giving follow-up to 13 months to reveal new bone formation, reduction of the periapical lesion and improvement in the color of the mucous membranes
Subject(s)
Humans , Female , Adult , Dental Amalgam/adverse effects , Mouth Mucosa/injuries , Pigmentation Disorders/diagnosis , Foreign-Body Reaction/physiopathology , Iatrogenic Disease , Diagnosis, Differential , Connective Tissue/surgery , Surgical FlapsABSTRACT
Objetivo Determinar los antígenos de Clase II en 52 pacientes con artritis reumatoide (AR) y 52 controles, y establecer la asociación de los antígenos de Clase II DRB1* con el factor reumatoide (FR), anticuerpos anti-péptido cíclico citrulinados (anti-CCP) y nódulos reumatoides. Material y Métodos Estudio de casos y controles, realizado en pacientes del Hospital Vicente Corral Moscoso y José Carrasco Arteaga de la ciudad de Cuenca-Ecuador; se incluyeron 52 pacientes con AR que cumplen los criterios del Colegio Americano de Reumatología (ACR) y 52 controles. Se identificaron a través de la Reacción en Cadena de la Polimerasa (PCR), los diferentes tipos de HLA que tienen cada uno de los 52 pacientes con AR y controles. Se buscó asociación de los HLA-DR1* con la AR y las pruebas de laboratorio: FR, anticuerpos anti-CCP y con nódulos reumatoides. El análisis estadístico se realizó con el cálculo de OR (IC 95%) y 2 de Pearson con corrección de Fisher y la información se procesó en SPSS v15. Resultados Los alelos más frecuentes fueron HLA-DRB1*09 (17,3%) OR 3,42 (IC95% 1,21 10,14) y HLADRB1*04 (32,7%) OR 1,81 (IC95% 0,93 3,55). Tanto el factor reumatoide como los anticuerpos anti-péptido cíclico citrulinados estuvieron presentes en el 82,7% de pacientes con artritis reumatoide y los nódulos reumatoides se detectaron en el 19,2% de pacientes con AR. Se encontró asociación entre los nódulos reumatoides con valores altos de FR y anti-CCP y estos a su vez con los alelos HLA-DRB1*14 y HLADRB1*04. La mayoría de pacientes con alelos HLA-DRB1*09 con predominio heterocigoto tuvieron anticuerpos anti-CCP y FR positivo. Conclusión La presencia de los alelos HLA-DRB1*04 y HLADRB1*09 está relacionada con la susceptibilidad de presentar AR en pacientes ecuatorianos, además se relaciona con valores elevados de factor reumatoide y anticuerpos anti-péptido cíclico citrulinados.
Objective To determine Class II antigens in 52 patients with rheumatoid arthritis (RA) and 52 controls and establish the association of Class II antigens DRB1* with rheumatoid anti- cyclic citrullinated peptide (anti-CCP) rheumatoid factor (RF), and antibodies rheumatoid nodules. Methods and Materials Case and control study in patients from the Hospital Vicente Corral Moscoso and José Carrasco Arteaga of Cuenca-Ecuador; 52 patients with rheumatoid arthritis who satisfy the criteria of ACR-American College of Rheumatology and 52 healthy patients. The different types of HLA from each 52 patients with rheumatoid arthritis and controls were identified through PCR - Polymerase Chain Reaction. Association of HLA-DR1* with rheumatoid arthritis and rheumatoid factor, anti-citrullinated and rheumatoid nodules. The statistical analysis was done by the OR (IC 95%) and 2 of Pearson with Fisher correction and the information was processed in SPSS v15. Results The most frequency alleles were HLA-DRB1*09 (17,3%) OR 3,42 (IC95% 1,21 10,14) and HLADRB1*04 (32,7%) OR 1,81 (IC95% 0,93 3,55). The rheumatoid factor and anti-cyclic citrullinated peptide antibodies were presented in 82,7% of patients with rheumatoid arthritis and rheumatoid nodules was detected in the 19.2% of patients with rheumatoid arthritis . An association between rheumatoid nodules with high values of rheumatoid factor and anti-ccp and these in turn with HLA-DRB1*14 y HLA-DRB1*04 alleles were found. The majority of patients with HLA-DRB1*09 alleles with heterozygote predomination had positive anti-ccp and rheumatoid factor. Conclusion The presence of HLA-DRB1*04 and HLA-DRB1*09 alleles is related with the susceptibility of present rheumatoid arthritis in Ecuadorian patients, and it is related with higher values of rheumatoid factor and anti-cyclic citrullinated peptide.
Subject(s)
Humans , Female , Adult , Middle Aged , Aged , Aged, 80 and over , Arthritis, Rheumatoid , Rheumatoid Factor , HLA Antigens , Rheumatoid Nodule , Anti-Citrullinated Protein Antibodies , AntibodiesABSTRACT
OBJECTIVES: To determine whether circulating gelsolin (GSN) levels in patients with ankylosing spondylitis (AS) undergoing TNF-α antagonist-infliximab-therapy are altered compared with controls and to establish whether disease activity, systemic inflammation and metabolic syndrome are potential determinants of circulating GSN levels in these patients. METHODS: We assessed GSN serum concentrations in a series of 30 non-diabetic AS patients without cardiovascular (CV) disease undergoing TNF-α antagonist-infliximab therapy and 48 matched controls. GSN levels were measured immediately before and after an infliximab infusion. Correlations of GSN serum levels with disease activity, systemic inflammation and metabolic syndrome were assessed. Potential changes in GSN concentration following an infusion of anti-TNF-α monoclonal antibody-infliximab were also analysed. RESULTS: Although at the time of the study AS patients undergoing anti-TNF-α therapy had adequate control of the disease (mean BASDAI 2.94), they showed lower GSN serum levels than healthy controls (mean±SD: 38660.42±23624.6 ng/ml versus 68975.43±31246.79 ng/ml; p<0.0001). When AS patients were stratified according to sex, we observed that GSN levels were significantly lower in men than in women (p=0.032). However, no differences in GSN levels according to the specific clinical features of the disease were seen. No association was found between GSN concentration and adipokines or biomarkers of endothelial cell activation. However, correlation between basal GSN levels and insulin resistance was observed. A single infliximab infusion did not lead to significant changes in GSN levels. CONCLUSIONS: GSN concentration is reduced in AS patients undergoing periodical anti-TNF-α therapy and low disease activity. Potential association with some metabolic syndrome features seems to exist.
Subject(s)
Antibodies, Monoclonal , Gelsolin/metabolism , Spondylitis, Ankylosing , Tumor Necrosis Factor-alpha/antagonists & inhibitors , Adipokines/metabolism , Adult , Antibodies, Monoclonal/administration & dosage , Antibodies, Monoclonal/adverse effects , Antirheumatic Agents/administration & dosage , Antirheumatic Agents/adverse effects , Dose-Response Relationship, Drug , Drug Monitoring , Female , Humans , Inflammation/drug therapy , Infliximab , Infusions, Intravenous , Male , Metabolism/drug effects , Middle Aged , Outpatients , Patient Acuity , Sex Factors , Spondylitis, Ankylosing/drug therapy , Spondylitis, Ankylosing/metabolism , Spondylitis, Ankylosing/physiopathology , Statistics as Topic , Treatment OutcomeABSTRACT
OBJECTIVES: To determine whether circulating osteopontin (OPN) levels in patients with ankylosing spondylitis (AS) undergoing TNF-α antagonist-infliximab-therapy are increased compared with controls and to establish whether disease activity, systemic inflammation, metabolic syndrome, adipokines and biomarkers of atherosclerosis are potential determinants of circulating OPN levels in these patients. METHODS: We assessed OPN serum concentrations in a series of 30 non-diabetic AS patients without cardiovascular disease undergoing TNF-α antagonist-infliximab therapy and 48 matched controls. OPN levels were measured immediately before and after an infliximab infusion, at time 0 and at time 120 minutes respectively. Correlations of OPN serum levels with clinical features, disease activity, systemic inflammation, metabolic syndrome and several biomarkers of atherosclerosis were assessed. Potential changes in OPN concentration following an infusion of anti-TNF-α monoclonal antibody-infliximab were also analysed. RESULTS: At the time of the study AS patients undergoing anti-TNF-α therapy had low disease activity (mean BASDAI 2.94) and they showed similar OPN serum levels to healthy controls. No differences in OPN levels according to the specific clinical features of the disease were seen. Also, no correlation between OPN concentration and insulin resistance and adipokines was observed. However, a positive correlation between OPN and angiopoietin-2 (Angpt-2) serum levels was found (r=0.397; p=0.04). In addition, a single infliximab infusion led to a marginal statistically significant reduction in OPN levels (24112.19±14608.73 pg/ml at time 0 versus 21806.62±11390.83 pg/ml at time 120'; p=0.05). CONCLUSIONS: OPN and Angpt-2 serum levels are correlated in non-diabetic AS patients undergoing TNF-α antagonist therapy.
