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1.
J Investig Med ; 59(3): 587-92, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21245771

ABSTRACT

BACKGROUND: Chronic obstructive pulmonary disease (COPD) is an inflammatory disease that is characterized by a progressive and irreversible decline in lung function and is caused primarily by chronic exposure to tobacco and to wood smoke. It is linked to oxidative stress and to an up-regulation of airway arginases and is also associated with alterations in platelets and erythrocytes. In the present study, arginase activity was studied in platelets and erythrocytes of 2 groups of COPD patients: 31 tobacco ex-smokers and 27 patients who had been exposed to wood smoke. A total of 15 healthy controls were also included. METHODS: Plasma, platelets, and erythrocytes were obtained from the blood samples. Levels of the oxidative stress biomarkers, carbonyls and malondialdehyde, were measured in the plasma, and arginase activity was quantified in platelets and erythrocytes. RESULTS: In both groups of COPD patients, an increase in the oxidative stress biomarkers was found. Platelet arginase activity in both COPD groups was 2-fold higher than that in the control group. In the erythrocytes, the arginase activity increased 1.7-fold over the control only in the wood smoke-induced COPD group. DISCUSSION AND CONCLUSIONS: These results suggest that the increase in arginase activity in platelets and erythrocytes participates in the alteration in nitric oxide metabolism in COPD patients and that there may be some differences between the tobacco smoke- and wood smoke-induced COPD.


Subject(s)
Arginase/metabolism , Blood Platelets/enzymology , Erythrocytes/enzymology , Pulmonary Disease, Chronic Obstructive/enzymology , Smoke/adverse effects , Smoking/metabolism , Aged , Aged, 80 and over , Biomarkers/metabolism , Case-Control Studies , Enzyme Activation/physiology , Female , Humans , Male , Middle Aged , Oxidative Stress/physiology , Pulmonary Disease, Chronic Obstructive/etiology , Smoking/adverse effects , Wood
2.
Front Biosci (Elite Ed) ; 2(4): 1385-93, 2010 06 01.
Article in English | MEDLINE | ID: mdl-20515811

ABSTRACT

Injury to red blood cell (RBC) membrane by oxidative stress is of clinical importance in chronic obstructive pulmonary disease (COPD) which leads to oxidative stress (OE) during disease progression. Here, we studied the impact of this stress on injury to RBC membrane. Blood samples from both healthy volunteers (HV, n = 11) and controlled COPD patients (n=43) were divided according to their GOLD disease stage (I=7, II=21, III=10, IV=5). Plasma levels of paraoxonase (PON) activity, protein carbonyls (PC), conjugate dienes, lipohydroperoxides (LPH) and malondialdehyde (MDA) were determined and the PTPase, and the oxidative parameters were measured in RBC ghosts. Plasma from patients with COPD showed an increased oxidation of lipids and proteins, that correlated with the disease progression. PON activity decreased from GOLD stages II to IV and correlated with an increase in LPH (p less than 0.0001, r = -0.8115). There was evidence of an increase in the oxidative biomarkers in RBCs, while the PTPase activity was diminished in stage III and IV of COPD. In conclusion, OE-induced injury associated with COPD is associated with an oxidative damage to the RBC membrane, with a concomitant decrease in the PTPase activity and altered function of anionic exchanger (AE1).


Subject(s)
Anion Exchange Protein 1, Erythrocyte/metabolism , Biomarkers/blood , Erythrocyte Membrane/metabolism , Protein Tyrosine Phosphatases/metabolism , Pulmonary Disease, Chronic Obstructive/blood , Disease Progression , Erythrocyte Membrane/enzymology , Humans , Pulmonary Disease, Chronic Obstructive/pathology
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