Subject(s)
Dracunculiasis/complications , Paraplegia/etiology , Adult , Dracunculiasis/diagnostic imaging , Humans , Male , Middle Aged , RadiographyABSTRACT
Motility related to gastrojejunostomy were studied in seven dogs which were prepared with a gastric cannula and platinum electrodes placed in the antrum, duodenum, jejunum and ileum. Five dogs were equipped with gastrojejunostomy at the time of electrode implantation and the two remaining dogs were used as control. Myoelectric activity recorded revealed several abnormal findings and occasionally spike activity occurred simultaneously in the duodenum, jejunum and ileum or it retropropagated from lower ileum to reach the stomach. The study suggests that the dyspeptic symptoms observed in patients after gastrojejunostomy may be associated with similar motility abnormalities as observed in these dogs.
Subject(s)
Gastric Bypass , Gastrointestinal Motility , Animals , Dogs , Electrodes, ImplantedABSTRACT
Detached smooth muscle cells from rat gastric antrum were isolated by incubation with collagenase and examined for viability and motility. Each stomach yielded about 10(7) muscle cells and were judged as intact and viable by the trypan blue dye exclusion. It is concluded that isolated smooth muscle cells of the rat gastric antrum by the technique of incubation with collagenase are judged viable and suitable for characterization of receptors on the surface membranes of these cells.
Subject(s)
Muscle, Smooth/cytology , Animals , Cell Survival , Cells, Cultured , Pyloric Antrum/cytology , RatsABSTRACT
An isolated ex vivo perfused mammalian stomach preparation is an ideal model for the study of motility avoiding central nervous influence and circulating humoral factors. In this paper, we describe the technique of such preparation in two different species: rat and cat, and its implication for motility study. While the isolated stomach was perfused with Krebs-Ringer solution via celiac artery, motility of the antrum was recorded using an open-tip tube in rats and a bipolar platinum electrode and a strain gauge in cats. The spontaneous antral motility and its response to drugs, such as dopamine and domperidone proved that the preparation would be a useful model to study motility devoid of influences of the central nervous system and circulating humoral agents.
