ABSTRACT
PURPOSE: To determine the effects of end-to-side nerve repair performed only with fibrin glue containing nerve growth in rats. METHODS: Seventy two Wistar rats were divided into six equal groups: group A was not submitted to nerve section; group B was submitted to nerve fibular section only. The others groups had the nerve fibular sectioned and then repaired in the lateral surface of an intact tibial nerve, with different procedures: group C: ETS with sutures; group D: ETS with sutures and NGF; group E: ETS with FG only; group F: ETS with FG containing NGF. The motor function was accompanied and the tibial muscle mass, the number and diameter of muscular fibers and regenerated axons were measured. RESULTS: All the analyzed variables did not show any differences among the four operated groups (p>0.05), which were statistically superior to group B (p<0.05), but inferior to group A (p>0.05). CONCLUSION: The end-to-side nerve repair presented the same recovery pattern, independent from the repair used, showing that the addition of nerve growth factor in fibrin glue was not enough for the results potentiating.
Subject(s)
Fibrin Tissue Adhesive/therapeutic use , Nerve Growth Factor/therapeutic use , Nerve Regeneration/drug effects , Peroneal Nerve/drug effects , Tissue Adhesives/therapeutic use , Anastomosis, Surgical/methods , Animals , Male , Nerve Endings/drug effects , Nerve Endings/physiology , Nerve Regeneration/physiology , Peroneal Nerve/injuries , Rats , Rats, WistarABSTRACT
PURPOSE: To determine the effects of end-to-side nerve repair performed only with fibrin glue containing nerve growth in rats. METHODS: Seventy two Wistar rats were divided into six equal groups: group A was not submitted to nerve section; group B was submitted to nerve fibular section only. The others groups had the nerve fibular sectioned and then repaired in the lateral surface of an intact tibial nerve, with different procedures: group C: ETS with sutures; group D: ETS with sutures and NGF; group E: ETS with FG only; group F: ETS with FG containing NGF. The motor function was accompanied and the tibial muscle mass, the number and diameter of muscular fibers and regenerated axons were measured. RESULTS: All the analyzed variables did not show any differences among the four operated groups (p>0.05), which were statistically superior to group B (p<0.05), but inferior to group A (p>0.05). CONCLUSION: The end-to-side nerve repair presented the same recovery pattern, independent from the repair used, showing that the addition of nerve growth factor in fibrin glue was not enough for the results potentiating.
OBJETIVO: Determinar os efeitos do reparo nervoso término-lateral realizado apenas com cola de fibrina contendo fator de crescimento nervoso em ratos. MÉTODOS: Setenta e dois ratos Wistar foram distribuídos em seis grupos: A - não submetido à secção nervosa; B - secção do nervo fibular (sem reparo); Os outros grupos tiveram o nervo fibular seccionado e então reparado na superfície lateral do nervo tibial intacto, com diferentes procedimentos: C - RNTL com suturas; D - RNTL com suturas e FCN; E - RNTL apenas com CF; F - RNTL com CF contendo FCN. A função motora foi acompanhada e a massa do músculo tibial, o número e o diâmetro das fibras musculares e axônios regenerados foram medidos. RESULTADOS: Não houve diferença entre as variáveis avaliadas nos quatro grupos operados (p>0,05), os quais foram superiores ao grupo B (p<0,05), mas inferiores ao grupo A (p>0,05). CONCLUSÕES: O reparo nervoso término-lateral mostrou o mesmo padrão de recuperação, independente do tipo de reparo utilizado, evidenciando que a adição de fator de crescimento nervoso na cola de fibrina não foi suficiente para a potencialização dos resultados.
Subject(s)
Animals , Male , Rats , Fibrin Tissue Adhesive/therapeutic use , Nerve Growth Factor/therapeutic use , Nerve Regeneration/drug effects , Peroneal Nerve/drug effects , Tissue Adhesives/therapeutic use , Anastomosis, Surgical/methods , Nerve Endings/drug effects , Nerve Endings/physiology , Nerve Regeneration/physiology , Peroneal Nerve/injuries , Rats, WistarABSTRACT
BACKGROUND: Mesenchymal chondrosarcoma (MC) is a subtype of chondrosarcoma, with an incidence varying from 1 to 8% of all chondrosarcomas. It is an aggressive neoplasm with a high tendency for late recurrence and occasional delayed distant metastasis. Orbital MC is very rare, and only approximately 30 cases have been described in the literature. We describe here one case of primary orbital MC. CASE REPORT: A 14-year-old boy without a past medical history presented with a 1-month history of progressive proptosis on the right eye. Computed tomography (CT) scans of the orbit revealed a right intraconic lesion, with areas of calcification. The lesion was excised. Histopathological analysis revealed that the tumor had a biphasic pattern, showing a combination of small cell malignancy and well-differentiated cartilage. Immunohistochemistry examination revealed a diffuse membrane expression of CD99 on the small cell malignancy; S-100 was positive only within the cartilage component. The patient received chemotherapy, and no metastatic disease was found at the 2-month follow-up. CONCLUSION: Although rare, MC should be considered in the differential diagnosis of a well-circumscribed orbital lesion in young adults, especially when CT scans reveal areas of calcification within the tumor.
Subject(s)
Chondrosarcoma, Mesenchymal/diagnosis , Orbital Neoplasms/diagnosis , 12E7 Antigen , Adolescent , Antigens, CD/analysis , Antigens, CD/biosynthesis , Calcinosis/diagnosis , Cartilage/metabolism , Cartilage/pathology , Cell Adhesion Molecules/analysis , Cell Adhesion Molecules/biosynthesis , Chondrosarcoma, Mesenchymal/metabolism , Chondrosarcoma, Mesenchymal/pathology , Chondrosarcoma, Mesenchymal/therapy , Diagnosis, Differential , Drug Therapy , Humans , Immunohistochemistry , Male , Mesenchymal Stem Cells/metabolism , Mesenchymal Stem Cells/pathology , Orbital Neoplasms/metabolism , Orbital Neoplasms/pathology , Orbital Neoplasms/therapy , S100 Proteins/analysis , S100 Proteins/biosynthesis , Tomography, X-Ray Computed , Treatment OutcomeABSTRACT
Familial primary localized cutaneous amyloidosis (FPLCA) is an autosomal-dominant disorder associated with chronic skin itching and deposition of epidermal keratin filament-associated amyloid material in the dermis. FPLCA has been mapped to 5p13.1-q11.2, and by candidate gene analysis, we identified missense mutations in the OSMR gene, encoding oncostatin M-specific receptor beta (OSMRbeta), in three families. OSMRbeta is a component of the oncostatin M (OSM) type II receptor and the interleukin (IL)-31 receptor, and cultured FPLCA keratinocytes showed reduced activation of Jak/STAT, MAPK, and PI3K/Akt pathways after OSM or IL-31 cytokine stimulation. The pathogenic amino acid substitutions are located within the extracellular fibronectin type III-like (FNIII) domains, regions critical for receptor dimerization and function. OSM and IL-31 signaling have been implicated in keratinocyte cell proliferation, differentiation, apoptosis, and inflammation, but our OSMR data in individuals with FPLCA represent the first human germline mutations in this cytokine receptor complex and provide new insight into mechanisms of skin itching.
Subject(s)
Amyloidosis, Familial/genetics , Oncostatin M Receptor beta Subunit/genetics , Amino Acid Sequence , Amyloidosis, Familial/pathology , Brazil , Cell Culture Techniques , Chromosomes, Human, Pair 5 , DNA Mutational Analysis , Female , Genes, Dominant , Humans , Keratinocytes , Male , Molecular Sequence Data , Mutation, Missense , Oncostatin M Receptor beta Subunit/chemistry , Pedigree , Sequence Homology , South Africa , United KingdomABSTRACT
Os autores relatam um caso de angiomixoma agressivo em paciente do sexo masculino, no cordão espermático. Essa entidade é descrita predominantemente em mulheres adultas, acometendo as regiões perineal, genital e pélvica. Macroscopicamente observou-se massa lobulada, infiltrativa, de limites imprecisos, que microscopicamente era representada por estroma mixóide com células pequenas, fusiformes e/ou estreladas, uniformes, sem figuras de mitose evidentes. Chamou atenção a presença de vasos sangüíneos proeminentes, de variados tamanhos, alguns deles com paredes espessadas. O perfil imuno-histoquímico mostrou positividade focal para desmina e actina de músculo liso (1A4) e negatividade para CD34 e CD68. Muitas neoplasias mixóides, tanto benignas quanto malignas, podem ser confundidas com o angiomixoma agressivo. O diagnóstico diferencial deve ser amplamente estudado, pois essa neoplasia tem caráter infiltrativo, alto índice de recorrência, embora não haja metástases relatadas até o presente momento.
