ABSTRACT
The toxic effects of an avermectin-impregnated fine plant powder (AIFP) against larval Aedes aegypti L. (Diptera: Culicidae), Culex modestus Ficalbi (Diptera: Culicidae), and Anopheles messeae Falleroni (Diptera: Culicidae), as well as selected nontarget aquatic invertebrates, were studied under laboratory conditions. The possibility of trophic transfer of avermectins (AVMs) through the food chain and their toxic effects on predaceous species fed AIFP-treated mosquito larvae was also evaluated. Among mosquitoes, Anopheles messeae were the most sensitive to AIFP, while Cx. modestus exhibited the least sensitivity to this formulation. Among nontarget aquatic invertebrates, the greatest toxicity of AIFP was observed for benthic species (larval Chironomus sp. Meigen (Diptera: Chironomidae), whereas predators (dragonflies, water beetles, and water bugs) exhibited the lowest AIFP sensitivity. AIFP sensitivity of the clam shrimp Lynceus brachyurus O. F. Muller (Diplostraca: Lynceidae), the phantom midge Chaoborus crystallinus De Geer (Diptera: Chaoboridae), and the mayfly Caenis robusta Eaton (Ephemeroptera: Caenidae) was intermediate and similar to the sensitivity of the mosquito Cx. modestus. However, these nontarget species were more resistant than An. messeae and Ae. aegypti. Solid-phase extraction of mosquito larvae treated with AIFP and subsequent high-performance liquid chromatography (HPLC) analysis of the extracts revealed an AVM concentration of up to 2.1 ± 0.3 µg/g. Feeding the creeping water bug Ilyocoris cimicoides L. (Hemiptera: Naucoridae) on the AIFP-treated mosquito larvae resulted in 51% mortality of the predaceous species. But no toxicity was observed for Aeshna mixta Latreille (Odonata: Aeshnidae) dragonfly larvae fed those mosquito larvae. The results of this work showed that this AVM formulation can be effective against mosquito larvae.
Subject(s)
Aquatic Organisms/drug effects , Culicidae/drug effects , Ivermectin/analogs & derivatives , Aedes/drug effects , Animals , Anopheles/drug effects , Culex/drug effects , Ecological Parameter Monitoring , Ephemeroptera/drug effects , Food Chain , Insecticides/pharmacology , Insecticides/toxicity , Invertebrates/drug effects , Ivermectin/pharmacology , Ivermectin/toxicity , Larva/drug effects , Mosquito Control , Odonata/drug effects , Powders/pharmacologyABSTRACT
Granules composed of alfalfa and sunflower meal that were impregnated with avermectins (AVMs) were developed for use against the Moroccan locust, Dociostaurus maroccanus (Thunberg). Laboratory experiments with granules containing 0.15% of AVMs fed to locust nymphs resulted in 100% death within 5 d. The quantification of AVM loss after exposure of AVM-containing preparative forms to UV light for various time periods was performed using high-performance liquid chromatography (HPLC). The results showed no loss of AVMs from the granules after 3 h of their exposure to UV light. The effect of UV radiation on a thin layer of the AVM solution led to the rapid degradation of AVMs. Only 0.2% of the initial AVM amount was detected after 3 h of exposure. In the granulated form, the AVM content remained stable for 10 mo when stored at room temperature in the dark. A method combining solid-phase extraction with HPLC was developed for the quantification of AVMs in locust nymphs. The granulated AVMs are characterized by their high resistance to UVB radiation. The use of plant-based granules impregnated with AVMs can be considered a very promising tool for locust control.
