The effects of cold stress on Mytilus species in the natural environment.

Environmental stressors induce changes in marine mussels from molecular (e.g., neurotransmitter and chaperone concentration, and expression of immune- and heat-shock protein-related genes) to physiological (e.g., filtration and heart rates, the number of circulating hemocytes) levels. Temperature directly affects the biogeographic distribution of mussels. Chaperones might form an essential part of endogenous protective mechanisms for the adaptation of these animals to low temperatures in nature. Here, we review the available studies dealing with cold stress responses of Mytilidae family members in their natural environment.

Chemical modulation of apoptosis in molluscan cell cultures.

This study focused on the alterations that occur in larval molluscan cells after administration of apoptotic inducers and inhibitors used in mammalian cells in response to cold stress. This is the first report on apoptosis modulation in molluscan cells assessed by flow cytometry. Mitochondrial activity, general caspase activation, and membrane integrity of control molluscan cells were compared to those processes in frozen-thawed molluscan cells, primary mouse embryonic fibroblasts, and human colon tumor cells prior to treatment and after incubation with apoptotic inducers or inhibitors. We tested three apoptotic inducers (staurosporine, camptothecin, and mitomycin C, routinely used for the chemical induction of apoptosis in different mammalian cells) and found that only staurosporine resulted in an evident apoptotic increase in molluscan cell cultures: 9.06% early apoptotic cells in comparison with 5.63% in control frozen-thawed cells and 20.6% late apoptotic cells in comparison with 10.68% in controls. Camptothecin did not significantly induce molluscan cell apoptosis but did cause a slight increase in the number of active cells after thawing. Mitomycin C produced similar results, but its effect was less pronounced. In addition, we hypothesize that the use of the apoptotic inhibitors could reduce apoptosis, which is significant after cryopreservation in molluscan cells; however, our attempts failed. Development in this direction is important for understanding the mechanisms of marine organisms' cold susceptibility.