ABSTRACT
PURPOSE: Gene expression patterns provide detailed insights into cellular regulation that reflect minor differences of cellular capacity not accessible by standard descriptions of the cellular phenotype or origin. METHODS: To identify fundamental differences and similarities we analyzed the gene expression patterns of four breast cancer cell lines: MCF-7, SK-BR-3, T-47D, and BT-474. RESULTS: Although only a small subset of genes (597) is represented on the Atlas-cDNA-Array (Clontech) used, clear differences in the expression of a number of genes could be detected. For example, unique high levels of expressions were found for the HLH-protein ID-1 (MCF-7) and the receptor tyrosine kinase erbB2 (SK-BR-3 and T-47D). Most genes analyzed were expressed at comparable levels in all cell lines studied. CONCLUSIONS: For interpretation of the results sets of genes that show similar variation of expression among the cells were grouped together. Furthermore, our analysis allows the assignment of similarity values that lead to a relation profile of the cell lines. How these results correlate with known biological properties of the cell lines is discussed. Additionally, we demonstrate that results obtained by cDNA-Array hybridization for expression of the ErbB receptor family correlate well with competitive RT-PCR, thus confirming the reliability of the cDNA-Array analysis.
Subject(s)
Breast Neoplasms/genetics , Gene Expression Profiling , Gene Expression Regulation, Neoplastic , Oligonucleotide Array Sequence Analysis , Repressor Proteins , Breast Neoplasms/pathology , DNA Primers , DNA, Complementary/genetics , DNA-Binding Proteins/biosynthesis , ErbB Receptors/biosynthesis , Female , Humans , Inhibitor of Differentiation Protein 1 , Receptor, ErbB-4 , Reverse Transcriptase Polymerase Chain Reaction , Transcription Factors/biosynthesis , Tumor Cells, CulturedABSTRACT
Ultrastructural and immunocytochemical results of studies on interneuronal relationship in vegetative ganglia were represented. Age-related changes of synapses (appearance of synaptic type vesicles and smooth endoplasmic reticulum cisterns in postsynaptic terminals), peculiarities of interneuronal links in neurons containing neuropeptide Y and NO-synthase and structural manifestations of non-synaptic interneuronal links were shown. Modern views on interneuronal communications were discussed.
Subject(s)
Ganglia, Autonomic/ultrastructure , Interneurons/ultrastructure , Aging/metabolism , Animals , Dogs , Ganglia, Autonomic/metabolism , Immunohistochemistry , Interneurons/metabolism , Male , Microscopy, Electron , Neuropeptide Y/metabolism , Nitric Oxide Synthase/metabolism , Rats , Rats, Inbred SHR , Rats, Sprague-Dawley , Rats, WistarABSTRACT
Immunohistochemical investigation of NO-synthase in brain astrocytic tumors revealed intense reaction in many tumor cells as well as direct correlation in the intensity of reaction and the degree of tumor anaplasia. Grade I astrocytomas did not show immunoreactivity in contrast to high anaplastic tumors where many cells had positive reaction with a different degree of intensity. Positive immunoreaction was shown in many giant cells. Small cell glioblastomas and oligodendrogliomas were immunonegative. There was a direct correlation between NO-synthase expression and glial fibrillar acidic protein.
