ABSTRACT
Spondylodiscitis caused by Campylobacter species is a rare disease which is most often caused by Campylobacter fetus. We report a case of culture-negative spondylodiscitis and a psoas abscess due to Campylobacter jejuni in a 68-year-old woman, as revealed by 16S rRNA gene and Campylobacter-specific PCRs from biopsied tissue.
Subject(s)
Campylobacter Infections/diagnosis , Campylobacter jejuni/genetics , Discitis/diagnosis , Aged , Campylobacter Infections/microbiology , Discitis/microbiology , Female , Humans , Molecular Diagnostic Techniques , Polymerase Chain Reaction , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNAABSTRACT
Visceral pentastomiasis caused by Armillifer armillatus larvae was diagnosed in 2 dogs in The Gambia. Parasites were subjected to PCR; phylogenetic analysis confirmed relatedness with branchiurans/crustaceans. Our investigation highlights transmission of infective A. armillatus ova to dogs and, by serologic evidence, also to 1 human, demonstrating a public health concern.
Subject(s)
Dog Diseases/transmission , Parasitic Diseases, Animal/transmission , Parasitic Diseases/transmission , Pentastomida/physiology , Snakes/parasitology , Zoonoses/transmission , Adult , Animal Husbandry , Animals , Antibodies/blood , Child , Dog Diseases/diagnosis , Dog Diseases/parasitology , Dogs , Female , Gambia , Humans , Male , Middle Aged , Molecular Sequence Data , Ovum/physiology , Parasitic Diseases/diagnosis , Parasitic Diseases/parasitology , Parasitic Diseases, Animal/diagnosis , Parasitic Diseases, Animal/parasitology , Pentastomida/genetics , Pentastomida/growth & development , Pentastomida/immunology , RNA, Ribosomal, 18S/genetics , Sequence Analysis, DNA , Zoonoses/parasitologyABSTRACT
The objective of this study was to characterize the antimicrobial resistance patterns of 100 clinical isolates of Enterobacter spp. with special regard to the occurrence of extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated quinolone resistance by qnr-determinants. The rate of ESBL- and qnr-positive isolates was 7% and 14%, respectively. Thirteen isolates harbored a qnrA1, and a further isolate harbored a qnrB4 gene. Moreover, qnr-determinants were significantly associated with ESBL-expression. No carbapeneme or tigecycline resistance was detected in the collective tested. To conclude, these data confirm the increase of multiple antimicrobial resistance mechanisms in Enterobacter spp.
Subject(s)
Anti-Bacterial Agents/pharmacology , Enterobacter/drug effects , Quinolones/pharmacology , beta-Lactamases/genetics , Bacterial Proteins/genetics , Drug Resistance, Bacterial/genetics , Enterobacter/genetics , Enterobacter/isolation & purification , Enterobacteriaceae Infections/drug therapy , Enterobacteriaceae Infections/microbiology , Germany/epidemiology , Humans , Microbial Sensitivity Tests , PlasmidsABSTRACT
Skin and soft tissue infections caused by Panton-Valentine leukocidin-producing strains of Staphylococcus aureus are emerging among travelers returning from the tropics. Here, we here present data on 15 affected individuals. Intrafamiliar spread was documented in one case, and occupational transmission was assumed in another. Spa typing of the strains revealed a broad spectrum of variants, but some were clonally related. Methicillin-resistant Staphylococcus aureus (MRSA) was found in three cases.
Subject(s)
Bacterial Toxins/metabolism , Exotoxins/metabolism , Leukocidins/metabolism , Soft Tissue Infections/microbiology , Staphylococcal Skin Infections/microbiology , Staphylococcus aureus/metabolism , Travel , Adult , Child , Communicable Diseases, Emerging , Female , Humans , Male , Methicillin-Resistant Staphylococcus aureus/isolation & purification , Middle Aged , Soft Tissue Infections/epidemiology , Staphylococcal Skin Infections/epidemiology , Staphylococcus aureus/classification , Staphylococcus aureus/genetics , Staphylococcus aureus/isolation & purification , Tropical ClimateABSTRACT
Tobramycin and colistin represent 2 standard antimicrobial agents in the treatment of cystic fibrosis (CF) patients who are chronically colonized with Pseudomonas aeruginosa. In this study, we determined the rate of resistance to tobramycin and colistin in 1844 isolates of P. aeruginosa obtained from 22 CF patients under alternate therapy with inhaled tobramycin and colistin. Resistance to tobramycin was observed in 27.5% of isolates. In contrast, all isolates were susceptible to colistin. Molecular typing of selected isolates suggested that only 1 clone occurred over time in each patient. To conclude, resistance to tobramycin in P. aeruginosa isolates from CF patients under antimicrobial therapy may occur while colistin resistance remains uncommon.
Subject(s)
Anti-Bacterial Agents/pharmacology , Colistin/pharmacology , Cystic Fibrosis/complications , Drug Resistance, Bacterial , Pseudomonas Infections/microbiology , Pseudomonas aeruginosa/drug effects , Tobramycin/pharmacology , Adult , Bacterial Typing Techniques , Chronic Disease , Cluster Analysis , Cystic Fibrosis/drug therapy , DNA Fingerprinting , Female , Genotype , Humans , Male , Pseudomonas Infections/drug therapy , Pseudomonas aeruginosa/classification , Pseudomonas aeruginosa/genetics , Pseudomonas aeruginosa/isolation & purificationABSTRACT
A total of 489 clinical isolates of Pseudomonas aeruginosa was investigated for metallo-beta-lactamase (MBL) production. Molecular analysis detected a blaVIM-1 gene in the chromosome of one isolate and a blaVIM-2 gene carried on the plasmid in seven isolates. Moreover, we showed that an initial screening by combined susceptibility testing of imipenem and ceftazidime followed by a confirmatory EDTA combination disk test represents a valid alternative to the molecular investigation of MBL genes, making MBL detection possible in routine diagnostic laboratories.
