Bioactive Compounds, Antioxidant Activities, and HPLC Analysis of Nine Edible Sprouts in Cambodia.

The non-nutritional health benefits of sprouts are unconfirmed. Thus, nine sprout methanolic extracts were tested for phytoconstituents and antioxidant activity. The TPC, TCC, TFC, TAC, and TALC were measured. ABTS and DPPH radical scavenging and ferric-reducing antioxidant power assays were used to assess the antioxidant activity. HPLC detected gallic acid, vanillin, syringic acid, chlorogenic acid, caffeic acid, and rutin in the extracts. The sprout extracts contained six compounds, with caffeic acid being the most abundant. Gallic acid, syringic acid, chlorogenic acid, caffeic acid, vanillin, and rutin were highest in soybean, black sesame, mustard, sunflower, white radish, and black sesame sprouts, respectively. Sunflower sprouts had the highest level of TCC while soybean sprouts had the highest level of TFC, Taiwanese morning glory had the highest level of TPC, mustard sprouts had the highest level of TALC, and black sesame sprouts had the highest level of TAC. Taiwanese morning glories scavenged the most DPPH and ABTS radicals. Colored and white radish sprouts had similar ferric-reducing antioxidant power. Antioxidation mechanisms varied by compound. Our findings demonstrated that sprouts have biological effects, and their short time for mass production offers an alternative food source for health benefits, and that they are useful for future research development of natural products and dietary supplements.

Assessment of ochratoxin A exposure risk from the consumption of coffee beans in Phnom Penh, Cambodia.

This survey aimed to determine OTA contamination in roasted coffee samples commercialised in Phnom Penh, Cambodia and to assess the potential health risk from OTA exposure. Forty locally grown and imported coffee samples were collected and analysed. Analytical validation methods were fully performed. In 3 of 40 samples (7.5%), the results showed detectable levels of OTA, ranging from 0.19 to 1.12 µg kg-1, with an overall average of 0.26 µg kg-1 and an average over the LOQ (n = 3) at 0.81 µg kg-1. OTA estimated daily intake (EDI) of both values were 0.05 (overall average) and 0.17 ng/kg bw/day (the worst-case scenario) with the calculated risk of OTA exposure expressed as a Hazard Quotient at 0.003 and 0.01, respectively. This result could imply a low health risk to Cambodian coffee consumers.