Activity of transcription factor JACKDAW is essential for SHR/SCR-dependent activation of SCARECROW and MAGPIE and is modulated by reciprocal interactions with MAGPIE, SCARECROW and SHORT ROOT.

Two GRAS family transcription factors, SHORT-ROOT (SHR) and SCARECROW (SCR), are required for ground tissue and quiescent center formation in Arabidopsis roots. The action of SHR and SCR is regulated by two INDETERMINATE DOMAIN (IDD) family proteins, JACKDAW (JKD) and MAGPIE (MGP). Although the reciprocal interaction of these transcription factors is considered to be involved in the modulation of SHR and SCR action by JKD and MGP, the underlying mechanism remains unclear. In this study, we use a transient assay with Arabidopsis culture cells to show that the physical interaction of these transcription factors modulate their transcriptional activity. Transient expression of LUC reporter genes with the proximal sequences upstream from the ATG codon of SCR and MGP in protoplasts were activated by JKD. Moreover, promoter activities were enhanced further by the addition of SHR and SCR to JKD, but not by the combination of SHR and SCR in the absence of JKD. Yeast one-hybrid analysis showed that JKD binds to the SCR and MGP promoter sequences, indicating the existence of another binding sequences of JKD different from the previously determined IDD binding sequence. Our findings suggest that JKD directly regulates SCR and MGP expression in cooperation with SHR, SCR and MGP.

Loneliness, depression and health status of the institutionalized elderly in Korea and Japan.

PURPOSE: The purpose of the study was to describe loneliness, depression, and health status in Korean and Japanese institutionalized elderly and explore differences between the countries. Also this study determined predictors of depression in each group. METHODS: Elderly subjects, aged 65-98 (n = 184), were recruited from private nursing homes in Korea and Japan. Subjects were interviewed on health status, loneliness, and depression. RESULTS: Korean subjects had higher loneliness scores than Japanese. More Korean elderly had depressive symptoms than Japanese elderly. The mean GDS score of Korean elderly was 8.07 and that of Japanese elderly was 5.21. Korean elderly had less physical function, and perceived their general health to be poor. Loneliness and perception of general health were significant predictors of depression in Korean and Japanese subjects. CONCLUSION: It is necessary to assess the levels of loneliness and depression of institutionalized Korean elderly and pursue an intervention to reduce these problems.

[Comparison of the literature on medical accidents from a medical database and articles in daily newspapers in Japan].

PURPOSE: We analyzed the frequency and content of journal reports on medical accidents over the past 15 years, and compared these findings with related articles in daily newspapers as a means of assessing medical professionals' awareness of current public opinion reflected in daily newspaper coverage. METHODS: A search of medical accident reports was conducted using CD-ROM versions of the medical literature database Japana Centra Revuo Medicina (1987-2001), with the key words "medical accident" and "medical error." The numbers of medical accident articles in newspapers and medical lawsuits were taken as indicators of public opinion. Relationships were investigated using correlation and time series analyses. RESULTS: 1) The total number of reports on medical accidents (including medical errors; hereinafter, medical accident reports) in the database was 2,858, equaling 0.78 medical accident reports for each 1,000 reports in the CD-ROM database. 2) The number of medical accident reports was 174 in 1987, gradually increasing to 333 in 2000 and 618 in 2001. 3) The proportion of the reports from the nursing field increased from 8.9% (1989) to 31.7% (1999). In contrast, the proportion concerning the medical field decreased from 68.8% to 50.2%. 4) The number of medical accident articles in two newspapers was investigated. The yearly trends were similar in both of these newspapers, showing an increase in 1999 and a peak in 2000. 5) A review of Supreme Court records revealed that the number of medical lawsuits each year increased gradually from 352 in 1990 to 805 in 2001. 6) There were significant correlations between the number of medical accident reports and the numbers of newspaper articles or medical lawsuits. A time series analysis of these trends showed that the trend in number of medical accident reports was closely linked with those for numbers of newspaper articles and medical lawsuits, with a time lag. CONCLUSION: Research trends in papers on medical accidents from a database of scientific papers were found to correspond with trend curves for medical lawsuits and articles on medical accidents in daily newspapers. It was thus demonstrated that awareness of medical accidents among medical professionals reflects social emphasis.