ABSTRACT
The identification of probiotic strain Lactobacillus sp. 55 at species level on the basis of analysis of the 16S rRNA gene nucleotide sequences has been done. It has been established that investigated strain belongs to the species Lactobacillus gasseri. The nucleotide sequence of strain Lactobacillus gasseri 55 16S rRNA gene's fragment has been deposited in international database GenBank (NCBI) under number 314159 KT.
Subject(s)
Lactobacillus/genetics , Phylogeny , Probiotics , Base Sequence , DNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNAABSTRACT
The composition of microflora of the distal part of intestine of 25 healthy and osteoporotic women-patients aged 50-79 years, depending on their diet, has been studied. The quantitative composition of intestinal microflora of women with osteoporosis, indicates the presence of dysbiosis caused by the decrease in the content of bifidobacteria and lactobacilli and a high level of undesirable microorganisms. The content of 78 nutrients in the diet of the examined women was analyzed. It was shown that the diet of patients with osteoporosis was more calorie rich and included a higher amount of essential nutrients than the diet of healthy women. The indicators of bone mineral density, depending on the number of representatives of nine groups of microorganisms isolated from the contents of the distal part of intestine, were analyzed. It has been well established that with an increase in the number of enterococci in the contents of the distal part of intestine the deterioration of the structural-functional state of the bone tissue can be observed.
