Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
Add more filters










Language
Publication year range
1.
Mem Inst Oswaldo Cruz ; 105(1): 25-32, 2010 Feb.
Article in English | MEDLINE | ID: mdl-20209325

ABSTRACT

The immunopathogenesis of chronic hepatitis C virus (HCV) infection is a matter of great controversy and has been suggested to involve a complex balance between cytokines with pro and anti-inflammatory activity. We investigated the expression of inflammatory cells and cytokines in the liver and serum of 51 chronically HCV infected patients and compared them to data from two sets of normal controls: 51 healthy blood donors and 33 liver biopsies of healthy liver donors. We also assessed the relationship between selected cytokines and cell populations in hepatic compartments and the disease stage. Compared with controls, hepatitis C patients had a greater expression of portal TNF-alpha, TGF-beta and CD4(+) and acinar IFN-gamma, TNF-alpha, IL-1beta and IL-4, as well as a higher serum concentration of IL-2, IL-10 and TGF-beta. Significant positive correlations were found between portal CD4+ and TNF-alpha, portal CD8(+) and TGF-beta, portal CD45(+)RO and TNF-alpha, acinar CD45(+)RO and IFN-gamma and acinar CD57(+) and TGF-beta. In conclusion, we have shown that (i) in this sample of predominantly mild disease, the immune response was associated with a pro-inflammatory response pattern, (ii) CD4(+) T-lymphocytes played a major role in orchestrating the immune response and (iii) these events primarily took place in the portal space.


Subject(s)
Cytokines/immunology , Hepatitis C, Chronic/immunology , Adolescent , Adult , Case-Control Studies , Female , Hepatitis C, Chronic/pathology , Humans , Immunity, Cellular , Immunohistochemistry , Male , Middle Aged , Severity of Illness Index , T-Lymphocytes/immunology , Young Adult
2.
Mem. Inst. Oswaldo Cruz ; 105(1): 25-32, Feb. 2010. tab, ilus
Article in English | LILACS | ID: lil-539292

ABSTRACT

The immunopathogenesis of chronic hepatitis C virus (HCV) infection is a matter of great controversy and has been suggested to involve a complex balance between cytokines with pro and anti-inflammatory activity. We investigated the expression of inflammatory cells and cytokines in the liver and serum of 51 chronically HCV infected patients and compared them to data from two sets of normal controls: 51 healthy blood donors and 33 liver biopsies of healthy liver donors. We also assessed the relationship between selected cytokines and cell populations in hepatic compartments and the disease stage. Compared with controls, hepatitis C patients had a greater expression of portal TNF-á, TGF-â and CD4+ and acinar IFN-ã, TNF-á, IL-1â and IL-4, as well as a higher serum concentration of IL-2, IL-10 and TGF-â. Significant positive correlations were found between portal CD4+ and TNF-á, portal CD8+ and TGF-â, portal CD45+RO and TNF-á, acinar CD45+RO and IFN-ã and acinar CD57+ and TGF-â. In conclusion, we have shown that (i) in this sample of predominantly mild disease, the immune response was associated with a pro-inflammatory response pattern, (ii) CD4+ T-lymphocytes played a major role in orchestrating the immune response and (iii) these events primarily took place in the portal space.


Subject(s)
Adolescent , Adult , Female , Humans , Male , Middle Aged , Young Adult , Cytokines/immunology , Hepatitis C, Chronic/immunology , Case-Control Studies , Hepatitis C, Chronic/pathology , Immunity, Cellular , Immunohistochemistry , Severity of Illness Index , T-Lymphocytes/immunology , Young Adult
3.
Clin Exp Dermatol ; 33(4): 472-7, 2008 Jul.
Article in English | MEDLINE | ID: mdl-18582233

ABSTRACT

BACKGROUND: Lymphocyte proliferation testing (LPT) has some advantages over patch testing to diagnose allergic contact dermatitis. It is harmless, objective and can be used in clinical situations where patch testing is not recommended. Unfortunately, significant success has only been achieved with nickel. There are few studies on chromium LPT and they were performed with different methods, leading to inconsistent results. METHODS: To determine the best parameters for chromium LPT, we tested 20 patients with allergic contact dermatitis to the metal and 20 controls, using various protocols. RESULTS: The best sensitivity and specificity ratios were achieved with 6-day cultures stimulated with a range from 7.5 x 10(-4) to 5 x 10(-3) mol/L of nonfiltered chromium chloride solutions. The sensitivity, specificity and accuracy values found within this range were 65%, 95% and 80%, respectively. CONCLUSION: Further investigation is necessary to achieve better sensitivity values.


Subject(s)
Chromium , Dermatitis, Allergic Contact/diagnosis , Patch Tests/methods , Adult , Chromium/adverse effects , Dermatitis, Allergic Contact/etiology , Dermatitis, Allergic Contact/immunology , Humans , Lymphocyte Activation , Male , Middle Aged , Sensitivity and Specificity
SELECTION OF CITATIONS
SEARCH DETAIL
...