Ubiquitin-conjugating enzyme V variant 1 enables cellular responses toward fibroblast growth factor signaling in endothelium.

Ubiquitination has been shown to provide an essential regulatory role in modulating the duration and amplitude of the signaling activity in angiogenesis. While successive enzymatic reactions mediated by three distinct types of enzymes commonly known as E1, E2, and E3 are required for ubiquitination, the role of E3s which govern the final step of ubiquitination has been extensively analyzed in angiogenesis. In contrast, the role of E2s, which determine the context and functional consequences of ubiquitination, remains largely unknown with respect to angiogenesis. To better elucidate the role of E2s in modulating endothelial behaviors during angiogenesis, we first systematically analyze the expression pattern of E2s in endothelial cells (ECs) using previously published scRNA-seq data and identify ubiquitin-conjugating enzyme variant 1 (UBE2V1), an unconventional E2 without innate catalytic activity, as one of the most abundantly expressed E2s in ECs. While ubiquitously expressed in diverse cell types, abrogation of UBE2V1 significantly impairs proliferation and viability of human umbilical vein endothelial cells (HUVECs) without affecting other cell types, suggesting that UBE2V1 is likely to possess nonredundant functions in ECs. Consistent with this idea, UBE2V1 appears to be critical for morphogenesis and migration of ECs during angiogenesis. Interestingly, we find that UBE2V1 is essential for fibroblast growth factor 2 (FGF2)-induced angiogenesis, but appears to have minor effects on vascular endothelial growth factor-A-induced angiogenesis in vitro as well as in vivo. Therefore, it seems that UBE2V1 could enable ECs to distinguish two related yet distinct angiogenic cues. Mechanistically, we show that UBE2V1 promotes ubiquitination of MEK kinase 1, a key mediator of FGF2 signaling, to enhance phosphorylation of extracellular signal-regulated kinase 1/2 in HUVECs. Taken together, our results illustrate the unique role of UBE2V1 as a key modulator for angiogenic behaviors in ECs.

A Comprehensive Survey on Security and Privacy for Electronic Health Data.

Recently, the integration of state-of-the-art technologies, such as modern sensors, networks, and cloud computing, has revolutionized the conventional healthcare system. However, security concerns have increasingly been emerging due to the integration of technologies. Therefore, the security and privacy issues associated with e-health data must be properly explored. In this paper, to investigate the security and privacy of e-health systems, we identified major components of the modern e-health systems (i.e., e-health data, medical devices, medical networks and edge/fog/cloud). Then, we reviewed recent security and privacy studies that focus on each component of the e-health systems. Based on the review, we obtained research taxonomy, security concerns, requirements, solutions, research trends, and open challenges for the components with strengths and weaknesses of the analyzed studies. In particular, edge and fog computing studies for e-health security and privacy were reviewed since the studies had mostly not been analyzed in other survey papers.

An Interoperable Access Control Framework for Diverse IoT Platforms Based on OAuth and Role.

Due to the rapid development of Internet of Things (IoT), IoT platforms that can provide common functions for things are becoming increasingly important. However, access control frameworks in diverse IoT platforms have been developed for individual security goals, designs, and technologies. In particular, current OAuth-based access control frameworks that are widely used in IoT research have not been providing interoperability among IoT platforms even though sharing resources and services is a critical issue for IoT platforms. Therefore, we analyze the main requirements for an IoT access control framework to properly design our framework and propose an interoperable access control framework based on OAuth 2.0 and Role. Our approach describes a new extended authorization grant flow to issue an Interoperable Access Token (IAT) that has a global access scope across IoT platforms using multiple pairs of clients' credentials. With the IAT and proposed framework, we can access client-specific domains in heterogeneous IoT platforms, then valuable resources (e.g., data and services) in the domains can be accessed by validating the roles, which will greatly simplify permission management. Furthermore, IAT supports a simple token management (e.g., token issuance, refreshing, and revocation) by managing only one token for diverse IoT platforms. In addition, we implement our interoperable access control framework on Mobius and FIWARE, which are promising open-source IoT platforms, and test an interoperability scenario to demonstrate our approach with the implementation. Furthermore, the proposed framework is compared with other IoT access control approaches based on the selected requirements in this paper.

Device Identification Interoperability in Heterogeneous IoT Platforms.

With the continuous improvement of Internet of Things (IoT) technologies, various IoT platforms are under development. However, each IoT platform is developed based on its own device identification system. That is, it is challenging to identify each sensor device between heterogeneous IoT platforms owing to the resource request format (e.g., device identifier) varying between platforms. Moreover, despite the considerable research focusing on resource interoperability between heterogeneous IoT platforms, little attention is given to sensor device identification systems in diverse IoT platforms. In order to overcome this problem, the current work proposes an IoT device name system (DNS) architecture based on the comparative analysis of heterogeneous IoT platforms (i.e., oneM2M, GS1 'Oliot', IBM 'Watson IoT', OCF 'IoTivity', FIWARE). The proposed IoT DNS analyzes and translates the identification system of the device and resource request format. In this process, resource requests between heterogeneous IoT platforms can be reconfigured appropriately for the resources and services requested by the user, and as a result, users can use heterogeneous IoT services. Furthermore, in order to illustrate the aim of the proposed architecture, the proposed IoT DNS is implemented and tested on a microcomputer. The experimental results show that a oneM2M-based device successfully performs a resource request to a Watson IoT and FIWARE sensor devices.

Inactivation of Paragonimus westermani metacercariae in soy sauce-marinated and frozen freshwater crabs.

Soy sauce-marinated freshwater crabs (Eriocheir japonicus) are a source of human paragonimiasis. The viability of Paragonimus westermani metacercariae (PwMc) in marinated crabs was investigated in an experimental setting. The PwMc collected from freshwater crayfish were inoculated into freshwater crabs, which were then frozen or marinated in soy sauce. All PwMc in the freshwater crabs were inactivated after freezing for 48 h at -20 °C and after freezing for 12 h at -40 °C. After marinating for 32 days, the survival rate of PwMc in 5% NaCl soy sauce was 50%, in 7.5% NaCl soy sauce it was 33.3%, and in 10.0% NaCl soy sauce it was 31.3%. When marinated for 64 days, all PwMc were inactivated in all experimental groups. These results revealed that freezing and soy sauce marination were detrimental to the survival of PwMc in freshwater crabs. Specifically, freezing crabs for more than 48 h or soaking them in soy sauce containing at least 5.0% NaCl for 64 days can inactivate PwMc. These results can inform the production of the traditional Korean soy sauce-marinated freshwater crabs known as gejang.

Evaluation of the removal and destruction effect of a chlorine and thiamine dilaurylsulfate combined treatment on L. monocytogenes biofilm.

The present study investigated the efficacy of single and combined treatment of both chlorine and thiamine dilaurylsulfate (TDS) on the reduction of Listeria monocytogenes biofilms in microtiter plate. The disinfectants used in this study were 50, 100, and 200 mg/L chlorine and 100, 500, and 1000 mg/L of TDS. Biofilm-forming index (BFI) and culturable cell count were used to evaluate the disinfectant assay. The highest BFI reduction was 0.80, achieved by the combination of 200 mg/L chlorine and 1000 mg/L TDS. In contrast, the highest culturable cell count reduction was 4.80 log colony-forming units/well by the combination of 200 mg/L chlorine and 100 mg/L TDS. The BFI was reduced in a concentration-dependent manner while culturable cell count was significantly reduced only when all chlorine concentration was combined with 100 mg/L TDS. However, when chlorine was combined with a higher concentration of TDS, the reduction decreased significantly. The result in this study showed that the combination of the 200 mg/L chlorine and 1000 mg/L TDS could be a practical application in removing L. monocytogenes biofilms from surfaces in food industry, and for the 200 mg/L chlorine and 100 mg/L, it can be used for killing the pathogen biofilms. However, more studies are still needed in order to show its efficacy on foods surfaces as well as to develop an even more effective treatment in both killing and removing biofilms.

Combined effects of chlorine and thiamine dilauryl sulfate on reduction of Listeria monocytogenes in chicken breast and development of predictive growth models.

The inhibitory effect of chlorine (50, 100, and 200 mL/kg) and thiamine dilauryl sulfate (TDS: 100, 500, and 1,000 mg/kg) on Listeria monocytogenes in chicken breast was investigated. Also, predictive growth models as a function of chlorine and TDS concentration, and storage temperature (4, 10, and 15°C) were developed using a polynomial model. Listeria monocytogenes counts were significantly (P < 0.05) different in samples treated with sterile distilled water and combinations of chlorine and TDS. The maximum reduction effect was 0.5 log cfu/g by combined treatment of 200 mL/kg chlorine and 1,000 mg/kg TDS. The largest synergistic effect was 0.38 log cfu/g by combined treatment of 100 mL/kg chlorine and 1,000 mg/kg TDS. The primary models that were developed to obtain the specific growth rates (SGR) and lag time (LT) had good fitness (R(2) > 0.91) determined by the reparameterized Gompertz equation. The secondary polynomial models were calculated by nonlinear regression analysis. In the validation of the developed models, the bias factor (Bf) and accuracy factor (Af) for SGR were 0.54 and 1.84, respectively, whereas those for LT were 0.97 and 1.04, respectively. In quality analysis, chlorine and TDS did not change the color or texture of chicken breast meat during storage at 4°C for 7 d. Thus, our findings indicate that a combined treatment of 100 mL/kg chlorine and 1,000 mg/kg TDS appears to an effective method into reduce L. monocytogenes in broiler carcasses with no negative effects on color and textural quality. The predictive models were in good agreement with the validation and may be used to predict L. monocytogenes growth in chicken breast.