ABSTRACT
We investigated the association of self-reported difficulty of food access, accounting for the perception of food-store accessibility, with nutrient intake among 474 inhabitants (145 men and 329 women) aged ≥40 y in a rural area of Japan. Information on self-reported difficulty of food access and dietary intake was obtained via a self-administrated questionnaire. Analysis of covariance was performed to evaluate the adjusted associations between difficulty of food access and percentages of total energy (i.e., protein, fat, and carbohydrates). Among men, the adjusted means of percent energy from fat in the "difficulty of food access" group (19.2% energy) were significantly lower (by 2.4%) and the percent energy from carbohydrates for this group (57.3% energy) was significantly higher (by 3.3%) than for the "non-difficulty" group. In conclusion, this study found nutritional balance among residents experiencing difficulty of food access results in lower fat and higher carbohydrate intake than for those with non-difficulty of food access among middle-aged and older Japanese men in a rural area.
Subject(s)
Diet , Dietary Carbohydrates/administration & dosage , Dietary Fats/administration & dosage , Feeding Behavior , Food Supply , Rural Population , Adult , Aged , Aged, 80 and over , Analysis of Variance , Energy Intake , Female , Humans , Japan , Male , Middle Aged , Self Report , Sex Factors , Surveys and QuestionnairesABSTRACT
The objective of this study was to develop an in-straw dilution method suitable for direct transfer of vitrified bovine sexed embryos. Embryo sexing was performed by molecular diagnosis. Several sexed and vitrified-warmed embryos were transferred after evaluation of morphologically embryonic survival at warming and in-straw dilution (Evaluation group). The other embryos were immediately directly transferred to recipients without first being expelled from the straws after in-straw dilution (Non-evaluation group). The pregnancy rates of vitrified sexed embryos were 38.7% and 34.8% in the Evaluation group and Non-evaluation group, respectively, which were not significantly different. The viability of lower quality embryos before vitrification tended to be lower (P = 0.087) than that of the higher quality embryos regardless of evaluating embryos after warming and in-straw dilution. The abortion rates were similar, and there was no difference between the two groups (13.9% and 12.5%, respectively). These results demonstrate that vitrified bovine sexed embryos can be vitrified and diluted by the in-straw method and that the vitrified and warmed sexed embryos can develop to term.
