Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 161
Filter
1.
J Orthop Surg (Hong Kong) ; 24(1): 45-50, 2016 04.
Article in English | MEDLINE | ID: mdl-27122512

ABSTRACT

PURPOSE: To determine the risk factors for elbow injury and its association with glenohumeral internal rotation deficit among young baseball players. METHODS: 229 baseball players aged 9 to 14 (mean, 11) years completed a self-administered questionnaire with items related to years of playing baseball, hours of training per weekday, days of training per week, and past and present experience of elbow pain. Two orthopaedic surgeons measured the range of motion of both shoulders and elbows. Another 2 orthopaedic surgeons performed ultrasonography to detect any elbow abnormality such as fragmentation of the medial epicondylar apophysis and osteochondritis dissecans of the capitellum. Using univariate and multivariable analyses, participants with or without elbow abnormality were compared to determine the risk factors for elbow abnormality. RESULTS: Elbow abnormality was detected in 100 of the participants and comprised osteochondritis dissecans of the capitellum (n=18) and fragmentation of the medial epicondylar apophysis (n=82). Elbow abnormality was associated with being a pitcher, past and present experience of elbow pain, loss of elbow extension, and the side-to-side internal rotation difference. The 100 participants with elbow abnormality were stratified into symptomatic (n=57) or asymptomatic (n=43) of elbow pain. Those with elbow abnormality and elbow pain was associated with being a pitcher. CONCLUSION: Being a pitcher was a risk factor for both elbow abnormality and elbow pain. Nonetheless, 43% of baseball players with elbow abnormality were asymptomatic. The use of ultrasonography was effective in detecting elbow abnormality and enabling early treatment.


Subject(s)
Baseball/injuries , Elbow Injuries , Elbow Joint/diagnostic imaging , Elbow/diagnostic imaging , Adolescent , Child , Humans , Male , Osteochondritis Dissecans/complications , Osteochondritis Dissecans/diagnostic imaging , Range of Motion, Articular , Risk Factors , Ultrasonography
2.
Tech Coloproctol ; 17(1): 51-7, 2013 Feb.
Article in English | MEDLINE | ID: mdl-22936589

ABSTRACT

BACKGROUND: The goals of this retrospective study were to comprehensively evaluate the impact of hepatic lymph node (HLN) involvement on survival in patients with synchronous resectable or unresectable liver metastases from colorectal cancer and to highlight how to deal with such cases in the light of recent advances in chemotherapy. METHODS: The impact of HLN involvement on survival, along with various clinical, pathological, and therapeutic factors, was retrospectively evaluated in 61 patients with synchronous liver metastases from colorectal cancer (resectable, 26; unresectable, 35), undergoing resection of the primary tumor and histopathological evaluation between July 2000 and April 2008. RESULTS: The proportion with HLN metastasis was 11.5 % in resectable cases and 28.6 % in unresectable cases. On multivariate analysis using the Cox proportional hazards model, HLN metastasis (P < 0.001), along with non-resection of hepatic lesions (P < 0.001), larger metastatic tumor volume (P < 0.001), non-use of oxaliplatin-based chemotherapy (P < 0.001), involvement of 4 or more regional lymph nodes (P < 0.001), and excessive lymphatic invasion (P = 0.02), was identified as an independent risk factor for shorter survival. CONCLUSIONS: To establish a new therapeutic strategy for synchronous liver metastasis of colorectal cancer, the HLNs should be examined histologically in patients undergoing resection of their primary colon and rectal cancer.


Subject(s)
Adenocarcinoma/secondary , Adenocarcinoma/surgery , Colorectal Neoplasms/pathology , Liver Neoplasms/secondary , Liver Neoplasms/surgery , Lymph Nodes/pathology , Adenocarcinoma/drug therapy , Adult , Aged , Aged, 80 and over , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Chemotherapy, Adjuvant , Colorectal Neoplasms/surgery , Female , Hepatectomy , Humans , Kaplan-Meier Estimate , Liver Neoplasms/drug therapy , Lymph Node Excision , Lymph Nodes/surgery , Lymphatic Metastasis , Male , Middle Aged , Multivariate Analysis , Neoplasm Invasiveness , Proportional Hazards Models , Retrospective Studies , Survival Rate , Time Factors
3.
Tech Coloproctol ; 15(3): 341-3, 2011 Sep.
Article in English | MEDLINE | ID: mdl-21655970

ABSTRACT

We report a new method of performing single-port laparoscopic surgery for appendiceal mucocele. The key points of our technique are placing a 3/4 circumferential skin incision with multiple radial splits on the confine of the umbilicus, use of a "home-made" multichannel port system, and trimming the skin incision straight through the confine of the umbilicus at the time of wound closure. A 65-year-old woman with appendiceal mucocele, 80 mm in diameter, successfully underwent ileocecal resection by this procedure. She remains in good health without any wound complications 8 months postoperatively.


Subject(s)
Appendiceal Neoplasms/surgery , Cystadenoma, Mucinous/surgery , Laparoscopy/methods , Aged , Female , Humans , Laparoscopy/instrumentation
4.
Tech Coloproctol ; 14(4): 311-5, 2010 Dec.
Article in English | MEDLINE | ID: mdl-20730550

ABSTRACT

BACKGROUND: We have been performing curative resection of colon cancer via a minilaparotomy without utilizing any laparoscopic instruments as an alternative to laparoscopic-assisted approach. Based on our experiences and improved surgical techniques, we have devised a new method for performing resection of stage 0/I colon cancer via a circumferential periumbilical skin incision that is associated with better cosmesis than standard minilaparotomy. METHODS: The short- and long-term results of curative colectomy via a circumferential periumbilical skin incision without utilizing any laparoscopic instruments performed in selected patients with stage 0/I colon cancer between October 2003 and July 2004 were analyzed. RESULTS: There were 8 men and 2 women with a median age of 66.5 years (range 61-77 years). Their median body mass index was 22.4 kg/m(2) (range 21.1-27.7 kg/m(2)). Pathological stage according the TNM classification was stage 0 in 4 patients and stage I in 6 patients. Median operative time was 160.5 min (range 135-203 min), and median blood loss was 60 ml (range 5-330 ml). Postoperative complications consisted of seroma in two patients and small bowel obstruction in one patient. After a median follow-up period of 5.7 years, there were no recurrences or wound complications. CONCLUSION: Curative colectomy via a circumferential periumbilical skin incision seems oncologically safe, yields satisfactory cosmetic results, and may provide an alternative to single-incision laparoscopic surgery in selected patients with colon cancer.


Subject(s)
Adenocarcinoma/surgery , Colectomy/methods , Colonic Neoplasms/surgery , Laparotomy/methods , Adenocarcinoma/pathology , Aged , Blood Loss, Surgical , Colectomy/adverse effects , Colonic Neoplasms/pathology , Female , Humans , Laparoscopy/methods , Male , Middle Aged , Neoplasm Staging , Postoperative Complications , Time and Motion Studies , Treatment Outcome , Umbilicus
5.
Tech Coloproctol ; 14(2): 153-9, 2010 Jun.
Article in English | MEDLINE | ID: mdl-20428914

ABSTRACT

BACKGROUND: This study evaluated the need for specific instruments when performing a curative resection of colon cancer via a minilaparotomy approach, which has been reported to be a minimally invasive alternative to a laparoscopic approach. METHODS: The feasibility, safety, and early oncological outcome were compared among 73 patients (first group), in whom a curative resection of colon cancer was performed via a minilaparotomy (skin incision < or =7 cm) utilizing specific instruments (North-bridge retractor system) between September 2002 and March 2005, and 94 patients (second group), in whom a similar procedure was performed without utilizing specific instruments between April 2005 and October 2007. RESULTS: The two groups did not differ significantly in terms of age, sex, body mass index, site of tumor, level of lymph node dissection, blood loss, UICC stage, number of harvested lymph nodes, incidence of postoperative complications, length of postoperative hospital days, or overall survival, although the frequency of prior abdominal surgery was higher (38.3 vs. 21.9%; P = 0.03) and the median operating time required for a standard lymph node dissection was shorter (120 vs. 135 min; P = 0.03) in the second group. CONCLUSION: With improved techniques and experience, specific instruments are not necessary for the performance of a curative colectomy via a minilaparotomy approach.


Subject(s)
Colectomy/instrumentation , Colonic Neoplasms/surgery , Laparotomy/instrumentation , Adult , Aged , Aged, 80 and over , Cohort Studies , Colectomy/adverse effects , Colectomy/methods , Colonic Neoplasms/mortality , Colonic Neoplasms/pathology , Feasibility Studies , Female , Humans , Laparotomy/adverse effects , Length of Stay , Male , Middle Aged , Survival Rate , Treatment Outcome
6.
Surg Endosc ; 19(3): 316-20, 2005 Mar.
Article in English | MEDLINE | ID: mdl-15624066

ABSTRACT

BACKGROUND: The early outcomes of minilaparotomy for resection of colonic cancer were evaluated. METHODS: In this study, 54 patients (34 Dukes' A, 15 Dukes' B, and 5 Dukes' C) successfully underwent curative resection of colonic cancer via minilaparotomy (skin incision, > or = 7 cm). The major exclusion criteria for this approach required a body mass index greater than 25 kg/m2, a tumor size exceeding 7 cm, a preoperative ileus, and tumor invading the adjacent organs. Patients (n = 54) who had undergone conventional open surgery before the introduction of this technique served as the control group by matching several clinicopathologic factors including body mass index. RESULTS: The passage of flatus (p < 0.01) and the beginning of oral intake (p = 0.02) were earlier, analgesic requirements were lower (p < 0.01), and postoperative serum C-reactive protein levels were lower in the minilaparotomy group (p < 0.01). The blood loss and frequency of postoperative complications did not differ between the groups. CONCLUSION: A minilaparotomy approach is a feasible, minimally invasive, and attractive alternative to conventional laparotomy for selected patients with colonic cancer.


Subject(s)
Colonic Neoplasms/surgery , Laparotomy/methods , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged
7.
Phys Rev Lett ; 93(16): 162701, 2004 Oct 15.
Article in English | MEDLINE | ID: mdl-15524983

ABSTRACT

Evaporation residue cross sections in the 16O+238U reaction were measured for the energy range from above- to extreme sub-barrier. The cross sections are reproduced by a statistical model calculation, for which partial cross sections are calculated by a coupled-channel model taking into account the prolate deformation of 238U. Complete fusion was observed in the collision of the projectile with the tips of the 238U target, in the same way as the side collision.

9.
Surg Endosc ; 17(6): 899-902, 2003 Jun.
Article in English | MEDLINE | ID: mdl-12618933

ABSTRACT

PURPOSE: We evaluated the data on initial experience of gasless laparoscopic surgery for patients with ulcerative colitis (UC) and familial adenomatous polyposis (FAP). PATIENTS AND METHODS: Seven patients (male/female = 3:4, median age 23, UC/FAP=5:2) underwent gasless laparoscopic total (procto) colectomy. Our basic surgical procedure involved (1) a 6- to 8-cm incision made at the beginning of the operation, (2) the wound pulled upward and/or laterally by retractors, and (3) conventional surgical instruments used through the wound; occasionally laparoscopic assistance and abdominal lifting were employed. The results were compared to those of 7 patients who had undergone conventional open surgery. RESULTS: Oral intake started earlier (p = 0.03) and C-reactive protein level on POD 4 was lower (p = 0.03) in the gasless group than in the control group. Duration of surgery, blood loss, requirement of analgesia, and morbidity rate were not significantly different between the groups. CONCLUSION: Our preliminary results suggest that gasless laparoscopic surgery for UC and FAP is feasible and can be an alternative method for minimally invasive surgery.


Subject(s)
Adenomatous Polyposis Coli/surgery , Colectomy/methods , Colitis, Ulcerative/surgery , Gases/therapeutic use , Laparoscopy/methods , Adolescent , Adult , Female , Humans , Male , Middle Aged
10.
Int J Clin Pharmacol Res ; 22(2): 29-35, 2002.
Article in English | MEDLINE | ID: mdl-12503773

ABSTRACT

We investigated the pattern of changes in serum gastrin level produced by three H2-receptor antagonists (H2RA) in patients with gastric and duodenal ulcers between 1990 and 1999. The subjects were 51 patients (cimetidine: 18 patients; famotidine: 16 patients; ranitidine: 17 patients). The gastrin test (in the fasting and meal-stimulated states) was conducted during drug administration and on the fourth day after drug cessation. After cessation of the drug therapy, the fasting serum gastrin level was significantly lower than that during the drug therapy with the three H2RAs. Gastrin level in the fasting test was significantly higher during famotidine therapy than during cimetidine therapy (p = 0.0123). In the meal-stimulated gastrin test, the AUC of gastrin during treatment with H2RA treatment was significantly higher with famotidine than with cimetidine (p = 0.0024). The results indicate different patterns of change in the serum gastrin level in the fasting and meal-stimulated test according to the H2RA administered. Gastrin level was highest in patients administered famotidine and lowest among those administered cimetidine. The pattern of gastrin change in patients administered ranitidine was intermediate between famotidine and cimetidine.


Subject(s)
Cimetidine/pharmacology , Famotidine/pharmacology , Gastrins/blood , Histamine H2 Antagonists/pharmacology , Ranitidine/pharmacology , Adult , Anti-Ulcer Agents/pharmacology , Anti-Ulcer Agents/therapeutic use , Area Under Curve , Cimetidine/therapeutic use , Duodenal Ulcer/blood , Duodenal Ulcer/drug therapy , Famotidine/therapeutic use , Female , Histamine H2 Antagonists/therapeutic use , Humans , Male , Middle Aged , Multivariate Analysis , Ranitidine/therapeutic use , Receptors, Histamine H2/physiology , Statistics, Nonparametric , Stomach Ulcer/blood , Stomach Ulcer/drug therapy
11.
Genome ; 45(5): 956-62, 2002 Oct.
Article in English | MEDLINE | ID: mdl-12416629

ABSTRACT

Precise location and nature of each of 14 length mutations detected among chloroplast DNAs of Triticum-Aegilops species by RFLP analysis were determined at the nucleotide sequence level. Each mutation was compared with at least three non-mutated wild-type plastomes as standards. These 14 length mutations were classified into 4 duplications and 10 deletions. One duplication occurred in the small single-copy region close to the border of the inverted repeat, and the remaining 13 length mutations took place in the large single-copy region. All length mutations occurred in the intergenic regions, suggesting that these length mutations do not affect plastid gene expression. Saltatory replication was the cause of all duplications, whereas intramolecular recombination mediated by short direct repeats played a substantial role in the deletions. Recurrent occurrences of certain deletion events were found in some AT-rich regions, which constituted hot spots for deletion. Out of four hypervariable regions detected among the grass plastomes, two (downstream of rbcL and a tRNA gene accumulated region) were still active after differentiation of Triticum and Aegilops complex.


Subject(s)
DNA, Chloroplast/genetics , Mutation , Poaceae/genetics , Triticum/genetics , Base Sequence , Chromosome Mapping , DNA Transposable Elements , Polymorphism, Restriction Fragment Length , Sequence Deletion
12.
Gan To Kagaku Ryoho ; 28(11): 1624-7, 2001 Oct.
Article in Japanese | MEDLINE | ID: mdl-11707995

ABSTRACT

We investigated whether the efficacy of peroral doxifluridine and hepatic arterial 5-FU infusion on synchronous liver metastasis of colorectal cancer could be predicted based on the expression of thymidine phosphorylase (TP) and dihydropyrimidine dehydrogenase (DPD) in the primary colorectal lesions. Ten patients with synchronous liver metastasis of colorectal cancer were given doxifluridine (600-800 mg/body/day) orally and 5-FU (500 mg/body, once or twice a week) through the hepatic artery following resection of the primary lesions between June 1996 and July 2001. The levels of TP and DPD in the primary lesions were determined by an enzyme-linked immunosorbent assay. The level of TP, DPD, and the ratio of TP/DPD in patients with partial response (n = 4) were 89.8 +/- 30.0 U/mg protein, 23.5 +/- 25.7 U/mg protein, and 3.8 +/- 1.4, respectively, while those in patients with no response or progressive disease (n = 6) were 41.8 +/- 9.7 U/mg protein, 25.8 +/- 15.8 U/mg protein, and 2.2 +/- 1.6, showing significant difference (p < 0.01) in the level of TP between the groups. These results indicate that determining the level of TS in primary colorectal lesions may be useful for predicting the efficacy of this regimen for patients with synchronous liver metastasis of colorectal cancer.


Subject(s)
Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Colorectal Neoplasms/enzymology , Liver Neoplasms/drug therapy , Liver Neoplasms/secondary , Oxidoreductases/metabolism , Thymidine Phosphorylase/metabolism , Aged , Colorectal Neoplasms/pathology , Colorectal Neoplasms/surgery , Dihydrouracil Dehydrogenase (NADP) , Drug Administration Schedule , Female , Floxuridine/administration & dosage , Fluorouracil/administration & dosage , Hepatic Artery , Humans , Infusions, Intra-Arterial , Liver Neoplasms/mortality , Male , Middle Aged , Survival Rate
13.
Gan To Kagaku Ryoho ; 28(11): 1760-2, 2001 Oct.
Article in Japanese | MEDLINE | ID: mdl-11708028

ABSTRACT

Little is known about the histological effect of hepatic arterial infusion with degradable starch microspheres (DSM), adriamycin (ADM), and mitomycin C (MMC) on liver metastasis of colorectal cancer. We histologically examined hepatic metastases resected following this therapy and investigated their relation to macroscopic changes in size. Neither a histological nor macroscopic effect was found in 2 patients receiving this therapy only one time. A 57-year-old woman with a solitary liver metastasis who received this therapy repeatedly (five times every 3 to 4 weeks) subsequently showed a partial response. She underwent hepatic metastasectomy 5 months after the end of the therapy. Histological examination showed that the greater part of the metastatic lesion was composed of fibrous tissues without any viable cancer cells. In conclusion, it should be noted that repeated infusions of DSM.ADM.MMC for liver metastases of colorectal cancer can have a marked histological effect even though the lesion does not show a complete response.


Subject(s)
Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Colorectal Neoplasms/pathology , Liver Neoplasms/drug therapy , Liver Neoplasms/pathology , Starch/administration & dosage , Aged , Doxorubicin/administration & dosage , Female , Humans , Liver Neoplasms/secondary , Male , Middle Aged , Mitomycin/administration & dosage
14.
Gan To Kagaku Ryoho ; 28(6): 809-14, 2001 Jun.
Article in Japanese | MEDLINE | ID: mdl-11432349

ABSTRACT

Fluoropyrimidine therapy for elderly colorectal cancer patients remains controversial. Tumoral levels of thymidine phosphorylase (TP), dihydropyrimidine dehydrogenase (DPD), and the ratio of TP to DPD determined by an enzyme-linked immunosorbent assay were compared between colorectal cancer patients aged 75 or over (elderly group, n = 25) and those 74 years or less (control group, n = 87), in order to examine the characteristics of colorectal cancers in the elderly from the viewpoint of metabolic and anabolic pathways of fluoropyrimidines. The level of TP was 78.4 +/- 47.0 unit/mg protein in the elderly group and 82.4 +/- 70.9 unit/mg protein in the control group (p = 0.86). The level of DPD was 53.7 +/- 43.1 unit/mg protein in the elderly group and 52.6 +/- 37.7 unit/mg protein in the control group (p = 0.73). The ratio of TP to DPD was 2.0 +/- 1.2 in the elderly group and 1.8 +/- 0.9 in the control group (p = 0.44). These three parameters did not differ between the groups when divided according to Dukes' stage (Dukes' A.B versus Dukes' C.D). These results suggest that there are no age-specific characteristics in relation to conversion of fluoropyrimidines such as capecitabine and doxifluridine to 5-fluorouracil (FU) and degradation of 5-FU in colorectal cancers.


Subject(s)
Colorectal Neoplasms/enzymology , Oxidoreductases/analysis , Thymidine Phosphorylase/analysis , Age Factors , Aged , Aged, 80 and over , Colorectal Neoplasms/drug therapy , Dihydrouracil Dehydrogenase (NADP) , Enzyme-Linked Immunosorbent Assay , Female , Fluorouracil/metabolism , Humans , Male
15.
Cancer ; 91(9): 1791-6, 2001 May 01.
Article in English | MEDLINE | ID: mdl-11335905

ABSTRACT

BACKGROUND: Photodynamic therapy (PDT) is an effective treatment modality that allows selective destruction of malignant tumor cells. However, because of the difficulty in exposing deeper areas of tumors, the modality has strictly limited indications. In this study, the authors introduce a new method for delivering laser light to a three-dimensional, wide area with the purpose of improving the therapeutic value of PDT. METHODS: Three patients with squamous cell carcinoma of the tongue were treated with the present course. After administering porfimer sodium, laser-proof tubes were inserted through the tumor, and optical fibers were passed through the tubes. Pulse laser was emitted from the obliquely prepared fiber tip and distributed toward the target area while rotating and reciprocating the optical fiber. RESULTS: Two patients achieved a complete response without any complications or functional disabilities. The third patient, however, had a partial response and required surgery. CONCLUSIONS: The results indicate the capability of distributing laser light to an entire area of solid tumor. However, the ideal dose of laser light for the treatment of tumors remains unknown. Interstitial PDT will be more efficacious and reliable after the dosimetry is established.


Subject(s)
Carcinoma, Squamous Cell/drug therapy , Photochemotherapy , Tongue Neoplasms/drug therapy , Aged , Antineoplastic Agents/therapeutic use , Dihematoporphyrin Ether/therapeutic use , Female , Fiber Optic Technology , Humans , Male , Middle Aged , Optical Fibers , Treatment Outcome
16.
J Dermatol Sci ; 26(2): 100-5, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11378325

ABSTRACT

Protein gene product PGP9.5 is a neuron-specific ubiquitin C-terminal hydrolase. We found that it also has immunoreactivity in the hair follicle of the Wistar rat dorsal skin and its expression patterns change with the development and cycle. During the morphogenesis, the PGP9.5 was expressed in the hair germ and hair peg elongated from the epidermis, and became restricted in the outer root sheath as the development progressed. In catagen, however, the PGP9.5 was detected in the tailing epithelial strand of the regressing proximal follicle epithelium, and in the keratinocytes directly contacted with the club hair, but rarely in the outer root sheath. With the beginning of the anagen of the second hair follicle, the PGP9.5 was again expressed in the second hair germ, and in the keratinocytes surrounding the remaining club hair and of distal follicle of the first hair. These findings showed that PGP9.5 is not specific to the neuron but is also involved in the hair follicle, and should provide new insight into the development and regression of the hair follicle.


Subject(s)
Hair Follicle/enzymology , Hair Follicle/growth & development , Thiolester Hydrolases/metabolism , Animals , Animals, Newborn , Hair Follicle/chemistry , Hair Follicle/innervation , Immunohistochemistry , Molecular Weight , Morphogenesis , Neurons/enzymology , Neurosecretory Systems/chemistry , Neurosecretory Systems/enzymology , Neurosecretory Systems/growth & development , Rats , Rats, Wistar , Thiolester Hydrolases/chemistry , Ubiquitin Thiolesterase
17.
Vet Parasitol ; 96(1): 65-74, 2001 Mar 05.
Article in English | MEDLINE | ID: mdl-11182236

ABSTRACT

Fourteen cDNA clones encoding epitopes of proteins of Toxoplasma gondii feline enteroepithelial-stages parasites were isolated and expressed in Escherichia coli in an effort to determine the antigenecity of the parasites. Sequence analysis showed that four of the cDNA clones had a 930-bp open-reading frame encoding a product showing similarity to the 14-3-3 protein mRNA sequence.(1) Southern hybridization of DIG-labeled positive clone with T. gondii genomic DNA cleaved with EcoRI, BamHI and HindIII resulted in one or two bands in each case. In an immunofluorescence assay, polyclonal and monoclonal antibodies raised against the expressed protein showed strong reactivity with feline enteroepithelial-stages parasites and sporozoites. In a complementation assay in which a plasmid carrying the protein-coding region of the isolated cDNA was introduced into a Saccharomyces cerevisiae mutant, strain DS9-22, the expressed protein showed complementation of the function of the 14-3-3 protein in yeast transformants. These findings suggest that T. gondii parasites produce a protein showing partial homology with members of the 14-3-3 protein family and this protein is expressed in feline enteroepithelial-stages parasites.


Subject(s)
Cat Diseases/parasitology , Toxoplasma/genetics , Toxoplasmosis, Animal/parasitology , Tyrosine 3-Monooxygenase/genetics , 14-3-3 Proteins , Amino Acid Sequence , Animals , Antibodies, Monoclonal , Antigens, Protozoan/genetics , Antigens, Protozoan/immunology , Base Sequence , Blotting, Southern/veterinary , Cats/parasitology , DNA, Complementary/genetics , Epitopes/genetics , Epitopes/immunology , Female , Fluorescent Antibody Technique, Indirect/veterinary , Gene Library , Genetic Complementation Test/veterinary , Male , Mice , Molecular Sequence Data , Sequence Alignment , Sequence Analysis, DNA , Sequence Homology, Amino Acid , Specific Pathogen-Free Organisms , Toxoplasma/immunology , Toxoplasma/metabolism , Toxoplasmosis, Animal/blood , Toxoplasmosis, Animal/immunology , Tyrosine 3-Monooxygenase/biosynthesis , Tyrosine 3-Monooxygenase/immunology
18.
Acta Derm Venereol ; 81(5): 360-3, 2001.
Article in English | MEDLINE | ID: mdl-11800146

ABSTRACT

We describe a unique patient with mosquito-bite hypersensitivity who had extremely high titres of Epstein-Barr virus antibodies. For many years he developed intractable ulcers on the sites of mosquito-bite. Epstein-Barr virus infection was detected in almost all inflammatory cells in the ulcers and in the peripheral blood lymphocytes by using in situ hybridization to Epstein-Barr virus-encoded small ribonucleic acids and by polymerase chain reaction to Epstein-Barr virus DNA. The inflammatory cells in the ulcers were positive for T-cell marker. Our results suggest that the Epstein-Barr virus infection in T cells may participate in the pathogenesis of exaggerated mosquito hypersensitivity and in delayed healing of ulcers on the sites of mosquito-bite.


Subject(s)
Culicidae , Epstein-Barr Virus Infections/complications , Herpesvirus 4, Human/isolation & purification , Insect Bites and Stings/complications , Leg Ulcer/etiology , T-Lymphocytes/virology , Adolescent , Animals , DNA, Viral/isolation & purification , Epstein-Barr Virus Infections/immunology , Epstein-Barr Virus Infections/pathology , Herpesvirus 4, Human/genetics , Humans , In Situ Hybridization , Insect Bites and Stings/immunology , Insect Bites and Stings/pathology , Leg Ulcer/pathology , Leg Ulcer/virology , Male , Severity of Illness Index
19.
Nihon Ishinkin Gakkai Zasshi ; 41(4): 263-7, 2000.
Article in English | MEDLINE | ID: mdl-11064326

ABSTRACT

The first cases of tinea corporis with Arthroderma benhamiae in Japan are reported. A 7-year-old girl and a 30-year-old mother in Shimane prefecture suffered from dermatophyte infections on the neck, shoulder, arms and leg. Three isolates from the two patients and a rabbit by which they supposedly were infected, were identified as Trichophyton mentagrophytes. On the bases of mating tests using the tester strains of both the African race and the Americano-European race of A. benhamiae, they were identified as A. benhamiae African race mating type (-). Our results are the first to indicate that both races of A. benhamiae exist in Japan.


Subject(s)
Arthrodermataceae/isolation & purification , Dermatomycoses/transmission , Dermatomycoses/veterinary , Rodent Diseases/transmission , Adult , Animals , Arthrodermataceae/pathogenicity , Child , Dermatomycoses/microbiology , Female , Human-Animal Bond , Humans , Male , Middle Aged , Rabbits , Rodent Diseases/microbiology
20.
Gan To Kagaku Ryoho ; 27(12): 1879-82, 2000 Oct.
Article in Japanese | MEDLINE | ID: mdl-11086435

ABSTRACT

Dihydropyrimidine dehydrogenase (DPD) levels were determined by enzyme-linked immunosorbent assay in primary tumors and adjacent normal mucosa from 114 colorectal cancer patients, including 9 with synchronous liver metastases. The level of intratumoral DPD was 55.0 +/- 38.7 unit/mg protein (n = 114) and that of mucosal DPD was 55.0 +/- 38.7 unit/mg protein (n = 114). The ratio of intratumoral DPD to mucosal DPD was 1.8 +/- 0.8 in patients developing metachronous liver metastases (n = 7), 1.0 +/- 0.5 in patients without recurrence (n = 61), and 1.0 +/- 1.1 in patients with synchronous liver metastases (n = 31) (p = 0.01, metachronous liver metastasis group versus recurrence-free and synchronous liver metastasis groups). The levels of DPD were higher in primary lesions than in synchronous liver metastasis (n = 9, p < 0.05). These results indicate that: (1) degradation of 5-fluorouracil (5-FU) is enhanced in hepatic lesions more than in primary lesions, which is consistent with previous findings showing the therapeutic advantage of hepatic arterial infusion over intravenous infusion in relation to the treatment of 5-FU for liver metastases of colorectal cancer patients; and (2) predicting the effectiveness of hepatic arterial infusion of 5-FU for patients with metachronous liver metastases is difficult based on DPD determination of primary lesions alone.


Subject(s)
Colorectal Neoplasms/pathology , Liver Neoplasms/enzymology , Liver Neoplasms/secondary , Liver/enzymology , Oxidoreductases/analysis , Dihydrouracil Dehydrogenase (NADP) , Enzyme-Linked Immunosorbent Assay , Humans
SELECTION OF CITATIONS
SEARCH DETAIL
...