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1.
Intern Med ; 56(6): 701-706, 2017.
Article in English | MEDLINE | ID: mdl-28321074

ABSTRACT

Epstein-Barr virus (EBV)-associated lymphoproliferative disorders (LPDs) sometimes occur following Anti-thymocyte globulin (ATG) administration for allogenic stem cell transplantation but are rare in aplastic anemia (AA) patients. A 55-year-old woman with AA following ATG developed refractory fever and was diagnosed with EBV-LPD. She was successfully treated with weekly rituximab monotherapy; however, she developed EBV encephalitis. She was admitted to the intensive care unit and finally recovered from unconsciousness. EBV-LPD should be considered after ATG for AA when symptoms appear. Because EBV-LPD following ATG for AA can rapidly progress, weekly monitoring of EBV-DNA and early intervention may be necessary.


Subject(s)
Anemia, Aplastic/drug therapy , Antilymphocyte Serum/adverse effects , Encephalitis/etiology , Epstein-Barr Virus Infections/etiology , Herpesvirus 4, Human , Lymphoproliferative Disorders/etiology , Antilymphocyte Serum/therapeutic use , Female , Humans , Middle Aged
2.
Biochem Biophys Res Commun ; 348(1): 183-91, 2006 Sep 15.
Article in English | MEDLINE | ID: mdl-16876123

ABSTRACT

We report the characteristics of CD300LG, a member of the CD300 antigen like family. Its genomic structure is similar in both mouse and human, and at least four isoforms exist in both species. The amino acid sequence of the immunoglobulin (Ig) V like domain of CD300LG showed approximately 35% identity to those of the polymeric Ig receptor (pIgR) and Fcalpha/muR. Interestingly, mouse CD300LG proteins were uniquely expressed on capillary endothelium. Immunoelectron microscopy revealed that mouse CD300LG is localized on both apical and basolateral plasma membranes, as well as on intracellular vesicular structures, in the capillary endothelium. Transcytosis assays using polarized MDCK epithelial cells showed that CD300LG could be transcytosed bidirectionally. Furthermore, CD300LG exogenously expressed on HeLa cells could take up IgA2 and IgM, but not IgG. These results suggest that CD300LG might play an important role in molecular traffic across the capillary endothelium.


Subject(s)
Antigens, CD/metabolism , Endothelial Cells/metabolism , Receptors, Immunologic/metabolism , Amino Acid Sequence , Animals , Antigens, CD/genetics , Antigens, CD/immunology , Antigens, Surface/genetics , Antigens, Surface/metabolism , Cloning, Molecular , Endothelial Cells/immunology , HeLa Cells , Humans , Immunohistochemistry , Membrane Glycoproteins/genetics , Membrane Glycoproteins/metabolism , Mice , Molecular Sequence Data , Organ Specificity , Receptors, Immunologic/genetics , Receptors, Immunologic/immunology , Receptors, KIR , Sequence Alignment
3.
J Immunol ; 176(1): 43-51, 2006 Jan 01.
Article in English | MEDLINE | ID: mdl-16365394

ABSTRACT

The recently identified CXCL16 has dual functions as a transmembrane adhesion molecule and a soluble chemokine. In this study we found that CXCL16 mRNA and protein were expressed constitutively on the follicle-associated epithelium covering Peyer's patches (PPs), isolated lymphoid follicles, and cecal patches, but minimally on the villous epithelium in the murine gastrointestinal tract. The CXCL16 receptor CXCR6/Bonzo was constitutively expressed on subpopulations of CD4+ and CD8+ T cells isolated from PPs. The expression of CXCR6/Bonzo on the PP T cells was up-regulated after stimulation with anti-CD3 and anti-CD28 mAbs. The activated PP T cells showed chemotactic migration in response to the soluble N-terminal chemokine domain of CXCL16. Furthermore, the activated PP T cells selectively adhered to cells expressing murine CXCL16. To determine the physiological role of CXCL16 in GALT, we first carefully analyzed T cell distribution in PPs. T cells localized not only in the interfollicular region but also at a lesser frequency in the subepithelial dome (SED) and in the germinal center of lymphoid follicles. Consistently, the majority of the adoptive transferred activated T cells migrated into the SED and the interfollicular region. However, the neutralization of CXCL16 specifically reduced the migration of the adoptive, transferred, activated T cells into the SED of PPs. These data suggest that CXCL16 expressed on the follicle-associated epithelium plays an important role in the recruitment and retention of activated T cells in the SED and should, at least partially, be responsible for lymphocyte compartmentalization in GALT.


Subject(s)
Chemokines, CXC/biosynthesis , Gastric Mucosa/immunology , Intestinal Mucosa/immunology , Receptors, Scavenger/biosynthesis , T-Lymphocytes/immunology , Adoptive Transfer , Animals , CD4 Antigens/immunology , CD8 Antigens/immunology , Chemokine CXCL16 , Chemokine CXCL6 , Chemokines/immunology , Chemokines, CXC/immunology , Chemotaxis, Leukocyte/immunology , Flow Cytometry , Gene Expression , Gene Expression Profiling , Immunohistochemistry , In Situ Hybridization , Mice , Peyer's Patches/cytology , Peyer's Patches/immunology , Polymerase Chain Reaction , RNA, Messenger/analysis , Receptors, Scavenger/immunology , T-Lymphocytes/cytology
4.
DNA Res ; 12(2): 127-37, 2005.
Article in English | MEDLINE | ID: mdl-16303744

ABSTRACT

Follicle-associated epithelium (FAE) covering Peyer's patches contains specialized epithelial M cells that take up ingested macromolecules and microorganisms from the lumen of the gut by transcytosis. Using high-density oligonucleotide microarrays, we analyzed the gene expression profiles of FAE and M cells in order to characterize their cellular phenotypes. The microarray data revealed that, among approximately 14,000 genes, 409 were expressed in FAE at twofold or higher levels compared to the intestinal epithelial cells (IECs) of the villi. These included genes involved in membrane traffic, host defense and transcriptional regulation, as well as uncharacterized genes. Real-time PCR and in situ hybridization analyses identified three molecules, ubiquitin D (Ub-D), tumor necrosis factor receptor superfamily 12a (TNFRsf12a), and transmembrane 4 superfamily 4 (Tm4sf4), which were predominantly distributed throughout FAE, but were expressed little, if at all, in IECs. By contrast, transcripts of secretory granule neuroendocrine protein 1 (Sgne-1) were scattered in FAE, and were co-localized with Ulex europaeus agglutinin-1 (UEA-1)-positive cells. This clearly suggests that expression of Sgne-1 in the gut is specific to M cells. Such a unique pattern of gene expression distinguishes FAE and M cells from IECs, and may reflect their cellular phenotype(s) associated with specific functional features.


Subject(s)
Epithelial Cells/metabolism , Peyer's Patches/metabolism , Proteome/metabolism , Animals , Gene Expression Profiling , Intestinal Mucosa/metabolism , Lymphocytes/metabolism , Male , Mice , Mice, Inbred BALB C , Neuroendocrine Secretory Protein 7B2/biosynthesis , Oligonucleotide Array Sequence Analysis
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