Subject(s)
Consciousness Monitors , Electromyography , Heart Arrest/complications , Heart Arrest/therapy , Hypothermia, Induced , Aged , Androstanols/administration & dosage , Humans , Male , Neurologic Examination , Neuromuscular Nondepolarizing Agents/administration & dosage , Predictive Value of Tests , Recovery of Function , RocuroniumABSTRACT
In order to investigate the influence of delivery mode on biological inactive renin levels in fetal circulation, plasma inactive renin (PIR), plasma renin activity (PRA) and plasma total renin (PTR) were measured in umbilical venous blood samples of 21 infants delivered vaginally after spontaneous labor and of 9 infants delivered by elective cesarean section after the onset of labor. Biological renin activities were measured by bioassay. The PIR levels in infants delivered vaginally were significantly lower than those in infants delivered by cesarean section, while the PRA levels were the opposite. However, the PTR levels were not significantly different between the two groups. These results suggest that the plasma levels of biological inactive renin in infants delivered vaginally may decrease, probably due to its conversion to active renin in the second stage of labor.
Subject(s)
Cesarean Section , Delivery, Obstetric/methods , Enzyme Precursors/blood , Fetal Blood/enzymology , Renin/blood , Adult , Enzyme Activation , Female , Humans , Labor, Obstetric/physiology , PregnancySubject(s)
Ammonia/metabolism , Cholestasis/metabolism , Liver/metabolism , Adenosine Diphosphate/analysis , Adenosine Monophosphate/analysis , Adenosine Triphosphate/analysis , Ammonium Chloride/administration & dosage , Animals , Carbamoyl-Phosphate Synthase (Ammonia)/analysis , Disease Models, Animal , Humans , Male , Mitochondria, Liver/metabolism , Ornithine Carbamoyltransferase/analysis , RabbitsABSTRACT
Ornithine carbamoyltransferase (EC 2.1.3.3) activity was estimated by determining the amount of citrulline produced. The citrulline was colorimetrically measured by using a diacetylmonoxime-thiosemicarbazide reaction without deproteinization. The color complex which had the maximum absorbance at 515 nm was stable at room temperature in daylight for at least several hours. The proposed method was superior to other methods already reported in sensitivity, stability and simplicity.
Subject(s)
Ornithine Carbamoyltransferase/blood , Citrulline/analysis , Diacetyl , Drug Stability , Hemolysis , Hot Temperature , Humans , Jaundice/enzymology , Methods , Semicarbazides , SpectrophotometryABSTRACT
The effect of 4-methylumbelliferone (4-MU) on the oxidative metabolism of liver mitochondria and its relationship to choleretic action were investigated on 40 bile duct-ligated rats and 40 rats after biliary decompression. The oxidative metabolism of liver mitochondria fell rapidly to 50% of controls within 7 days after bile duct-ligation, whereas that in rats with 4-MU fell only slightly. Such effect of 4-MU on the liver mitochondrial metabolism was most prominent when it was administered to rats after biliary decompression. In rats with 4-MU the depressed oxidative metabolism of cholestatic liver mitochondria recovered rapidly toward normal with accelerated amelioration of serum total bilirubin as compared to that in rats without 4-MU after biliary decompression by loosening of the ligation of the common bile duct. Thus, 4-MU appeared to exert some stimulation or amelioration of the impaired liver mitochondrial metabolism due to jaundice, which may have a close relationship to its choleretic action or effective bile excretory action.
